Protein-DNA binding residues
Monoclonal Antibodies against the Minimal DNA-Binding Domain in the Carboxyl-Terminal Region of Human Immunodeficiency Virus Type 1 Integrase
... maltose-binding protein (MBP) and thereafter with HIVIN, with the N-terminal 20 aa residues containing a hexahistidine ...HiTrap protein A column (Amersham Pharmacia Biotech ...
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Essential role of the Vp2 and Vp3 DNA-binding domain in simian virus 40 morphogenesis.
... viral DNA by exchanging a fragment containing the DBD mutations with that of the wild- type SV40 DNA ...35 residues of Vp2/3 and the amino ter- minus of Vp1 are expressed in different reading frames ...
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Structure-Based Mutational Analysis of the C-Terminal DNA-Binding Domain of Human Immunodeficiency Virus Type 1 Integrase: Critical Residues for Protein Oligomerization and DNA Binding
... and DNA strand transfer, can be carried out in vitro with synthetic DNA oligo- nucleotide substrates which mimic the viral DNA ends, diva- lent metal ions, and purified recombinant IN ...retroviral ...
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Sequence similarity between the erythrocyte binding domain 1 of the Plasmodium vivax Duffy binding protein and the V3 loop of HIV 1 strain MN reveals binding residues for the Duffy Antigen Receptor for Chemokines
... pHVDR22 DNA, a plasmid kindly provided by ...gD protein [7] Duffy Fy (a-b+) erythrocytes were washed three times in RPMI 1640, resuspended to a hematocrit of 1% in 1 ml of complete DMEM with the chemokines ...
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Characterization of the Epstein-Barr virus BZLF1 protein transactivation domain.
... However, other features of Zta may be important for activity since a Gal4-Zta chimeric protein, generated by fusing the amino-terminal 167 residues of Zta to the DNA binding domain of th[r] ...
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Common determinants in DNA melting and helicase-catalysed DNA unwinding by papillomavirus replication protein E1
... of DNA binding, melting and unwinding by the pro- tein ...double-stranded DNA is threaded through the ...of DNA in the central channel, and its binding also appears to induce structural ...
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Surface lysine and tyrosine residues are required for interaction of the major herpes simplex virus type 1 DNA-binding protein with single-stranded DNA.
... 11 0022-538X/92/116273-07$02.00/0 Copyright © 1992, American Society for Microbiology Surface Lysine and Tyrosine Residues Are Required for Interaction of the Major Herpes Simplex Virus [r] ...
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A Highly Organized Structure Mediating Nuclear Localization of a Myb2 Transcription Factor in the Protozoan Parasite Trichomonas vaginalis
... non-nuclear protein, the Myb2 sequence spanning amino acid (aa) residues 48 to 143, which is embedded within the R2R3 DNA-binding domain (aa 40 to 156), was essential and sufficient for ...
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Identification of a promoter-specific transactivation domain in the herpes simplex virus regulatory protein ICP4.
... localization, DNA binding, and dimerization have been defined, but a domain that is specifically required for transactivation has not been ...139 residues of ICP4 and is significantly less potent ...
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The human T transcription factor: A study of genetics and function
... T protein in com plex with its DNA target (M üller and Herrmann, ...the protein, effectively the DNA binding domain, it was dem onstrated that T binds to a 24 bp palindrom ic DNA ...
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Surface Mutagenesis of the Bovine Papillomavirus E1 DNA Binding Domain Reveals Residues Required for Multiple Functions Related to DNA Replication
... bility is that these mutations affect some aspect of replication control, which is required for DNA replication in vivo but not in vitro. For example, it is likely that replication in vivo is controlled by ...
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S-nitrosation on zinc finger motif of PARP-1 as a mechanism of DNA repair inhibition by arsenite
... finger DNA repair ...in DNA binding, which is critically important to PARP-1 DNA recognition and DNA repair activity [27, ...Zinc binding to the PARP-1 zinc finger motif is ...
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DNA-binding domain of bovine papillomavirus type 1 E1 helicase: structural and functional aspects.
... A series of small internal deletions and substitution mutations in the DNA-binding domain of El show that specific basic residues in this region of the protein, which are conserved in al[r] ...
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Antibodies to Envelope Glycoprotein of Dengue Virus during the Natural Course of Infection Are Predominantly Cross-Reactive and Recognize Epitopes Containing Highly Conserved Residues at the Fusion Loop of Domain II
... Most studies of antibody responses after DENV infection employed assays that incorporated the whole DENV as anti- gen, such as the hemagglutination inhibition assay, enzyme- linked immunosorbent assay (ELISA), and ...
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Promyelocytic leukaemia protein isoform II and mild heat stress compromise human adenovirus type 5 gene expression
... GAL4- DNA Binding Domain (DBD) alone, reporter gene expression was greatly reduced when cells were transfected with GAL4-Daxx, and this reduction was rescued in a dose-dependent manner by co-expression of ...
235
Dominant-Negative Inhibition of Prion Formation Diminished by Deletion Mutagenesis of the Prion Protein
... 53. Shmerling, D., I. Hegyi, M. Fischer, T. Blattler, S. Brandner, J. Gotz, T. Rulicke, E. Flechsig, A. Cozzio, C. von Mering, C. Hangartner, A. Aguzzi, and C. Weissmann. 1998. Expression of amino-terminally truncated ...
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Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8).
... ICP8, the major DNA-binding protein from herpes simplex virus type 1 HSV-1, belongs to a class of singlestranded DNA-binding proteins.. These proteins are required for DNA replication, p[r] ...
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The DNA Binding Domain of a Papillomavirus E2 Protein Programs a Chimeric Nuclease To Cleave Integrated Human Papillomavirus DNA in HeLa Cervical Carcinoma Cells
... BEF-induced DNA double-strand break near E2 BS4, another high-affinity BS in integrated HPV18 DNA in HeLa ...a DNA double-strand break in the HPV18 DNA near E2 BS4 ...by DNA from cells ...
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Role of the Hemagglutinin-Neuraminidase Protein in the Mechanism of Paramyxovirus-Cell Membrane Fusion
... the binding/NA active pocket and the hydrophobic surface ...conserved residues in the binding/NA active site of HN, such as Arg174 (which lies on 1S1) and Lys236 (which lies on 1S2) underwent ...
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COMPARATIVE DOCKING AND MUTATIONAL STUDIES CKIT AND PDGFR A INHIBITORS IN GASTROINTESTINAL STROMAL TUMORS
... has protein sequence and functional ...studio protein preparation was done for cKIT and ...loaded protein, identifying the potential problems, automatically fixing, removing water molecules and other ...
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