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Protein Extraction

Protein Extraction from Spirulina Platensis

Protein Extraction from Spirulina Platensis

... The extraction of protein from green spirulina microalgae platensis was carried out by centrifugation, ultra sound assisted extraction and combination of centrifugation and ultrasound assisted ...

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Optimisation of ultrasonic assisted protein extraction from brewer’s spent grain

Optimisation of ultrasonic assisted protein extraction from brewer’s spent grain

... the protein yield. Four extraction parameters (extraction time, ultrasonic power, S/L, and pH value of extractant) were ...the protein yield was indicated in Figure 1. It was found that the ...

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Protein Extraction from Grape Seeds by Reverse Micelles: Optimization of the Forward Extraction

Protein Extraction from Grape Seeds by Reverse Micelles: Optimization of the Forward Extraction

... micelles extraction is a novel separation technology with prospect for separating ...of extraction conditions has been used in enhancing the yield of many proteins [17] [18] [19] [20] ...the ...

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Survey of Intracellular Protein Extraction Methods from Pichia pastoris

Survey of Intracellular Protein Extraction Methods from Pichia pastoris

... intracellular protein under different cell breaking conditions were investigated in this ...the protein release ...proteins extraction was 30% (wet weight, w/v) of yeast cells suspend in 50 mM ...

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Sweetpotato Peel Protein: Extraction Optimization and Susceptibility to Digestive Enzymes.

Sweetpotato Peel Protein: Extraction Optimization and Susceptibility to Digestive Enzymes.

... for protein quantification instead of Bradford assay, so the proposed optimum conditions do not appear to be affected by the lack of inter-assay ...underestimated protein in ...sweetpotato protein ...

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The Recovery of Protein from Egg Yolk Protein Extraction Granule Byproduct

The Recovery of Protein from Egg Yolk Protein Extraction Granule Byproduct

... A study by Eckert et al. (2014) examined the use of an unconventional proteinase from Asian pumpkin (Cucurbita ficifolia) to obtain bioactive peptides from the by- product of a phospholipid extraction from egg ...

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Extraction of Protein from Microalgae Using Low Pulsed Electric Field

Extraction of Protein from Microalgae Using Low Pulsed Electric Field

... The present study shows additional insight into understanding the PEF method of cell disruption for protein extraction from Chlorella vulgaris, Chlorococcum sp. and Scenedesmus sp.. FTIR spectra show ...

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Extraction, Characterization and Antioxidant Activity in vitro of Proteins from Semen Allii Fistulosi

Extraction, Characterization and Antioxidant Activity in vitro of Proteins from Semen Allii Fistulosi

... Proteins are ampholytes with positively and negatively charged groups, and their solubility varies with pH. Thus, the appropriate pH value is helpful to protein extraction. With respect to the effect of pH ...

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Not just fat : investigating the proteome of cetacean blubber tissue

Not just fat : investigating the proteome of cetacean blubber tissue

... thus showed no evidence of bloating and decomposition, were used in order to investigate blubber proteins that had not been subject to extensive degradation and metabolism after death. Samples showed no evidence of ...

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Membrane Protein Profiling of Human Islets of Langerhans Using Several Extraction Methods

Membrane Protein Profiling of Human Islets of Langerhans Using Several Extraction Methods

... different extraction methods ...individual protein extraction procedure showed that the highest number of proteins (1,136) was detected when analyzing total protein extracts of the islets ...

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2.	L.B. Mezajoug Kenfack, D. Tapimali Tsamé and C. Tchiégang

2. L.B. Mezajoug Kenfack, D. Tapimali Tsamé and C. Tchiégang

... through protein extraction is ...the protein of the two plants was studied (Mezajoug Kenfack, 2010), but the effect of phytate on the extraction was not ...on protein extraction ...

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Transfer and expression of the rabbit defensin NP-1 gene in lettuce (Lactuca sativa).

Transfer and expression of the rabbit defensin NP-1 gene in lettuce (Lactuca sativa).

... total protein from control and transgenic lettuce plants that showed a hybridization signal in the Southern blot was extracted using a Plant Protein Extraction Kit (ComWin, ...The protein ...

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Efficient extraction of proteins from formalin-fixed paraffin-embedded tissues requires higher concentration of tris(hydroxymethyl)aminomethane

Efficient extraction of proteins from formalin-fixed paraffin-embedded tissues requires higher concentration of tris(hydroxymethyl)aminomethane

... of extraction efficiency of proteins extracted by 100, 200, 300, 400, 500 and 600 mM TRIS in PEB at 90°C for 60 ...and extraction efficiency of those proteins didn’t reach at the ...TRIS-dependent ...

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Optimization of extraction of deoiled rice bran protein and its utilization in wheat based cookies

Optimization of extraction of deoiled rice bran protein and its utilization in wheat based cookies

... extract protein from rice bran. However, a large proportion of rice bran protein cannot be extracted by ordinary aqueous solvent because of its extensive disulfide bonds and ...for protein ...

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Extraction Methods and Functional Properties of Protein from Arthospira platensis for Bioavailability of Algal Proteins

Extraction Methods and Functional Properties of Protein from Arthospira platensis for Bioavailability of Algal Proteins

... Protein extraction from ...ultrasound extraction took place at 4 ± 1°C for 60 ...the protein content. 2.2. Determination of Soluble Protein Yield ...

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Review on the extraction of biomolecules by biosurfactant reverse micelles

Review on the extraction of biomolecules by biosurfactant reverse micelles

... on protein extraction by the reversed micelle process indicate that the difference between the pH and the isoelectric point (pI) of the protein must oscillate between 1 and 2 points for higher ...

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Protein profiling of Orthosiphon stamineus

Protein profiling of Orthosiphon stamineus

... its protein content (Basheer and Majid, 2010, Yam et ...of protein profile from ...total protein extraction from the plant to pave way for further proteomics ...

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Protein Protein Interaction Extraction Based on Convex Combination Kernel Function

Protein Protein Interaction Extraction Based on Convex Combination Kernel Function

... in Protein-Protein Interaction (PPI) extraction, which was made by different single kernel functions, and only using single kernel function hardly trained the optimal classified model to extract PPI, ...

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Value of hydroalcoholic treatment of rapeseed for oil extraction and protein enrichment☆

Value of hydroalcoholic treatment of rapeseed for oil extraction and protein enrichment☆

... oil extraction. Lipid extraction yield thus reached 91% with hexane and 83 – 84% with azeotrope alcohols ...lipid extraction yield to 46% and 66% for 92 ...

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The extraction of collagen protein from pigskin

The extraction of collagen protein from pigskin

... Using acid or alkali alone to treat chrome shavings can only give us elementary collagen protein products. However, using alternating acid-alkali method can result in improvement in de-chroming rate. Plus, ...

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