Proteomics And Mass Spectrometry
Towards high throughput and spatiotemporal proteomics : analytical workflows and quantitative label-free mass spectrometry
... MS-based proteomics is a widely-accepted universal methodology providing miscellaneous information on molecular mechanisms regulating cellular systems from the point of protein function, localization, modification ...
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Mass spectrometry-based proteomics techniques and their application in ovarian cancer research
... Classical proteomics such as LC-MS may be used to gener- ate a molecular fingerprint of disease [75, ...imaging mass spectrometry (MALDI-IMS) ...
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Statistical Methods for the Analysis of Mass Spectrometry-based Proteomics Data
... based proteomics, LC-MS, which has become the tool of choice for identifying and quantifying the proteome of an ...the mass spectrometer. In tandem mass spectrometry (denoted by MS/MS), ...
106
Contributions of mass spectrometry based proteomics to defining cellular mechanisms and diagnostic markers for systemic lupus erythematosus
... of proteomics using bronchoalveolar lavage fl uid as a sample for the study of sarcoidosis [59], systemic sclerosis [60,61], and other pulmonary diseases ...Other mass spectrometry-based tech- niques ...
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Development of ultrahigh resolution FTICR mass spectrometry methods for clinical proteomics
... Mass spectrometry- (MS-) based proteomics is a principal platform in systems biology, that is, the integrated approach of different technical disciplines to study the physiological processes in a ...
219
A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study
... analyze mass spec- trometry-based isotope-labeled datasets, targeting the two questions that typically arise in proteomics experi- ments: 1) which proteins are differentially regulated regarding the ...
19
Mass spectrometry-assisted gel-based proteomics in cancer biomarker discovery: approaches and application
... as mass spectrometry (MS), enable the identification of pathologically significant proteins in various types of ...MS-assisted proteomics has contributed to the development of cancer biomarkers that ...
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Untargeted analysis of the airway proteomes of children with respiratory infections using mass spectrometry based proteomics
... The upper airway – which consists mainly of the naso- and oro-pharynx - is the first point of contact between the respiratory system and microbial organisms that are ubiquitous in the environment. It has evolved highly ...
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Urinary proteomics of Henoch-Schönlein purpura nephritis in children using liquid chromatography-tandem mass spectrometry
... Urinary proteomics as a novel tool has become an efficient approach for predicting renal involvement prior to renal biopsy and biomarker discov- ery in kidney diseases ...dem mass spectrometry ...
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Mass Spectrometry-Based Proteomics Analysis of the Matrix Microenvironment in Pluripotent Stem Cell Culture
... Since proteins are the effectors of cellular processes, it is important to investigate how stem cells are regulated by changes at the protein level in addition to genomic analyses. Post-translational modifications (PTM), ...
388
Senescence marker protein 30 in acute liver failure: validation of a mass spectrometry proteomics assay
... The plasma proteomics protocol was as described in our previous study [5]. In brief, plasma samples were pooled and precipitated with cold acetone, then dissolved in buffer containing 7 M urea, 2 M thiourea, 4% ...
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Review Article Mass Spectrometry-Based Proteomics and Peptidomics for Biomarker Discovery in Neurodegenerative Diseases
... of mass spectrometry for the analysis of large biomolecules including proteins and ...as mass accuracy, resolving power, sensitivity and throughput should be taken into ...in proteomics [40]. ...
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A feasibility study to identify proteins in the residual Pap test fluid of women with normal cytology by mass spectrometry-based proteomics
... performed mass spectrometry- based proteomic analysis of cervical-vaginal fluid obtained using swabs, gauze, or Dacron-tipped plastic applicators (reviewed in ...for proteomics and biomarker ...
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Mass Spectrometry-Based Proteomics Reveals Distinct Mechanisms of Astrocyte Protein Secretion
... in mass spectrometry-based proteomics have enabled greater depth of analysis for the astrocyte secretome, allowing comparative analyses of secretomes under different conditions and the identification ...
238
Discovery and longitudinal evaluation of candidate biomarkers for ischaemic stroke by mass spectrometry-based proteomics
... quantitative proteomics on serum samples using high-resolution mass spectrometry and long high-performance liquid chromatography gradient (5 hours) combined with a 50-cm column to optimise the ...
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Mass spectrometry for translational proteomics: progress and clinical implications
... of mass spectrometry (MS)-based proteomic analyses and their clinical applications have been increasingly recognized over the past decade due to their high sensitivity, specificity and ...based ...
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YPED: An Integrated Bioinformatics Suite and Database for Mass Spectrometry-based Proteomics Research
... of mass spectrometry (MS) data in pro- teomics research has necessitated the creation of new bioinfor- matics tools and databases to efficiently pull together diverse sets of ...‘‘democratizing’’ ...
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In silico proteome analysis to facilitate proteomics experiments using mass spectrometry
... in proteomics experiments usually rely on measurement of ionized peptides in a mass ...electrospray mass spectrometry analysis of mouse liver and lung tissue and validated using the SEQUEST ...
15
Sparse Proteomics Analysis - a compressed sensing-based approach for feature selection and classification of high-dimensional proteomics mass spectrometry data.
... of an effect caused e.g. by a disease, we further sub-divided the case group into five sub-groups where the amount of spiked-in proteins is increasing. The five volumes in the case sub-groups were spiked with the ...
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Quantification of mutant SPOP proteins in prostate cancer using mass spectrometry based targeted proteomics
... mutations are distributed at seven distinct positions within a 49-AA region (AA 87–135) [2], requiring mul- tiple distinct probes to fully characterize mutant SPOP proteins. Moreover, there are multiple lysine residues ...
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