Quantitative PCR assay
Development of a real time quantitative PCR assay for detection of a stable genomic region of BK virus
... We proposed that identifying areas of the genome that are conserved within and between species could be used to supplement limited nucleotide sequence information when designing assays. Target regions identified via ...
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Development and Validation of a Quantitative PCR Assay for Diagnosis of Scedosporiosis
... real-time PCR (RT-PCR) assays for the diagnosis of these species were developed and validated for the classification of clinical strains and for the detection of DNA in clinical samples by use of a murine ...
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Internally Controlled Triplex Quantitative PCR Assay for Human Polyomaviruses JC and BK
... conventional PCR that differentiates the vi- ruses on the basis of amplicon size ...qPCR assay for JCPyV and BKPyV in clinical ...and PCR inhibition in clinical samples is consid- ered essential to ...
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Quantitative PCR assay for detection of Bois noir phytoplasmas in grape and insect tissue
... In Europe's vineyards "Bois noir" (BN) is an ex- panding yellows disease on Vitis vinifera. It is associated with phytoplasmas of the stolbur group (16SrXII-A). Two subtypes are important, one is associated with ...
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Serial Quantitative PCR Assay for Detection, Species Discrimination, and Quantification of Leishmania spp in Human Samples
... include quantitative analysis of parasites in buffy coat or serum specimens to document re- sponse to therapy, species determination in tissue biopsy spec- imens or tissues scraped from microscopic slides, and ...
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Real Time Quantitative PCR Assay for Monitoring of Nervous Necrosis Virus Infection in Grouper Aquaculture
... In real-time qPCR, a positive reaction is detected by accu- mulation of a fluorescent signal, which not only allows detec- tion of the presence of the viral genome but also enables an estimate of its infectious ...
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Keeping Pace with Parvovirus B19 Genetic Variability: a Multiplex Genotype Specific Quantitative PCR Assay
... our assay, single-base mismatches are present either on one of the primer binding sites or on the probe binding site, at a fre- quency lower than ...molecular assay in the different de- tection formats have ...
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Applicability of a Real Time Quantitative PCR Assay for Diagnosis of Respiratory Syncytial Virus Infection in Immunocompromised Adults
... or PCR either lack sensitivity, require complex laboratory manipulation, or have not been evaluated in this patient ...real-time PCR technique for the detection of RSV A and B in immunocompromised adults, ...
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Sensitive Detection of Multiple Rotavirus Genotypes with a Single Reverse Transcription Real Time Quantitative PCR Assay
... (RT)–real-time quantitative PCR ap- proach targeted to the rotaviral VP2 gene, based on a multiple-sequence alignment of different human rotaviral ...the assay was tested on isolates representing the ...
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Evaluation of a real-time quantitative PCR assay for diagnosis of Pneumocystis jirovecii pneumonia in immunocompromised patients: A Pilot study
... BG assay can be used as a screening test for PCP because it has high sensitivity for PCP rather than IFI, which will avoid the need of invasive samples for ...this assay has moderate specificity, if test ...
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A quantitative PCR (TaqMan) assay for pathogenic Leptospira spp
... real-time PCR, diagnosis Abstract Background: Leptospirosis is an emerging infectious ...immunosorbent assay (ELISA) and slide agglutination test (SAT), can detect different classes of antibody but may be ...
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Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... sensitive PCR methods are needed for diagnosis of this bacterium because it is very difficult to culture from patient ...a quantitative real-time LightCycler PCR was ...The assay could consis- ...
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Multiplex, Quantitative, Real Time PCR Assay for Cytomegalovirus and Human DNA
... multiplex, quantitative, real-time PCR assay that amplifies cytomegalovirus (CMV) and human DNA in the same reaction tube, allowing for a viral load determination that is normalized to measured human ...
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Development of a Sensitive Quantitative Competitive PCR Assay for Detection of Human Cytomegalovirus DNA
... the PCR. Regarding sensitivity, our PCR assay could detect about 10 copies, and measured DNA quantitatively from 10 to 100,000 copies in the extracts ...to PCR reaction did not cause a ...
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Clinical characterization of a competitive PCR assay for quantitative testing of hepatitis C virus
... of quantitative assessments of hepatitis C virus (HCV) RNA depends on an understanding of factors affecting the assay and its intrinsic ...available quantitative reverse transcriptase PCR ...
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Quantitative Fluorogenic PCR Assay for Measuring Ovine Herpesvirus 2 Replication in Sheep
... fluorogenic PCR specific for ovine herpesvirus 2 (OvHV-2) DNA was developed and compared to a pre- viously established conventional seminested ...fluorogenic PCR for genomic sheep DNA was required to ...
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Development and evaluation of a real time PCR assay for the quantitative detection of Theileria annulata in cattle
... species. PCR has been used for the sensitive and specific detec- tion of ...real-time PCR assay that can be used for both the detection and the quantification of ...
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A neutralization assay for respiratory syncytial virus using a quantitative PCR based endpoint assessment
... an assay that measures 90% inhibition within a single virus infection cycle as compared with an assay that measures 50% reduction in plaque counts following multiple cycles of virus ...
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Quantitative Analysis of Epstein Barr Virus Load by Using a Real Time PCR Assay
... tected PCR inhibitors and estimated the efficiency of the ex- traction methods, both of which are particularly important to quantify the EBV load accurately and ...conventional PCR assays, which have ...
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Novel Technique of Quantitative Nested Real Time PCR Assay for Mycobacterium tuberculosis DNA
... nested PCR techniques can be considered to be approximately equivalent and to be significantly higher than that of conventional single- step ...nested PCR and the QNRT-PCR assays revealed negative ...
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