quantitative polymerase chain reaction
Diagnosis of tubercular uveitis by quantitative polymerase chain reaction
... Purpose To report the use of real-time or quantitative polymerase chain reaction (qPCR) in confirming the diagnosis of tubercular uveitis. Methods A qPCR assay using primers targeting the ...
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Measurement of bioprocess containment by quantitative polymerase chain reaction
... However, the implications of GILSP and OECD containment category 1 in large scale manufacture using GMMOs is that some release is inevitable but is not likely to [r] ...
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Establishment of Micro Droplet Digital Polymerase Chain Reaction and Real-Time Fluorescence Quantitative Polymerase Chain Reaction Technologies for Detecting Zika Virus
... established a method for ZIKA nucleic acid detection using ddPCR and qPCR. Our results 206[r] ...
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Detection of human herpesvirus 8 by quantitative polymerase chain reaction: development and standardisation of methods
... While most primer/probe manufacturing companies can produce synthetic oligonucleotides these cannot be quantified, and the amplicon must be small (≤200 bp). As neither of these methods were ideal for our assays, nor are ...
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Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
... If variables such as the methods of mRNA extraction, RNA quality assessment, reverse transcription, assay design, meas- urement of genomic contamination, standard curve data gen- eration, reference gene selection, and ...
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Analysis of normal and osteoarthritic canine cartilage mRNA expression by quantitative polymerase chain reaction
... If variables such as the methods of mRNA extraction, RNA quality assessment, reverse transcription, assay design, meas- urement of genomic contamination, standard curve data gen- eration, reference gene selection, and ...
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Analysis of Specific Bacteria from Environmental Samples using a Quantitative Polymerase Chain Reaction
... 2 Laragen Inc., 10755 Venice Ave., Los Angeles, CA 90034, USA Abstract This article describes the use of quantitative PCR for measuring bacterial abundance in environmental samples. The two approaches discussed ...
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Antiadenoviral effects of ganciclovir in types inducing keratoconjunctivitis by quantitative polymerase chain reaction methods
... In this study, ganciclovir had a significant inhibitory effect in a dose-dependent manner against all selected types of HAdV – HAdV3, 4, 8, 19a, and 37 – which induce epidemic [r] ...
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Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... Multiplexing primers and probes into one reaction or 2 reactions have been published also (Chmielewicz B et al 2005; Gu Z et al 2003; Ebner K et al 2006; Ebner K et al 2005). All these assays have been proved to ...
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Antiadenoviral effects of <em>N</em>-chlorotaurine in vitro confirmed by quantitative polymerase chain reaction methods
... In our present study, the antiviral activity of NCT against HAdV4, species E, was weaker than that against other serotypes and significant dose-dependency was not found.. 22 A[r] ...
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Real-time quantitative PCR (polymerase chain reaction)- page 47
... Fig.28C: Relative fold change of CRBN mRNA level in peripheral blood (PB) mononuclear cells of female patient with with del(5q) and trisomy 8 in different clones during len[r] ...
New Developments in Quantitative Real-time Polymerase Chain Reaction Technology
... The net effect of such interferences, at least in RT-qPCR, is the invariable quenching of the fluorescent signal, leading to sub-optimal or irreproducible gene expression data. Consequently, solutions are constantly ...
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The efficacy of quantitative fluorescent-polymerase chain reaction (QF-PCR) in the diagnosis of prenatal aneuploidy
... aneuploidy Aim: To investigate the efficacy of quantitative fluorescent-polymerase chain reaction (QF-PCR) in the diagnosis of aneuploidy. Materials and methods: The study included 40 pregnant ...
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Gene expression of the renin angiotensin system in human tissues Quantitative analysis by the polymerase chain reaction
... the polymerase chain reaction (PCR) after reverse transcription to detect renin- angiotensinogen-, and angiotensin-converting enzyme-mRNA in small quantities of human ...
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Selection of suitable reference genes for quantitative real time polymerase chain reaction in human meningiomas and arachnoidea
... To validate the expression stability of CASC3, EIF2B1, IPO8, MRPL19, PGK1, POP4, PPIA, RPL37A plus GAPDH and ACTB, thirty-four randomly chosen pri- mary cultured meningiomas, the meningi[r] ...
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Reference genes for quantitative reverse transcription polymerase chain reaction expression studies in wild and cultivated peanut
... We have assessed the stability of ten candidate reference genes for qRT-PCR normalization using an entire data- set and eight samples subsets of leaves and roots from wild relatives and [r] ...
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Development of a fluorescent quantitative real time polymerase chain reaction assay for the detection of Goose parvovirus in vivo
... × 10 1 copies/reaction. Comparisons were made between the conventional PCR method and our established FQ- PCR method using dilution series of pVP3 plasmid DNA to calculate the end-point sensitivity of each assay. ...
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Quantitative Real-time Polymerase Chain Reaction for Tracking Microbial Gene Expression in Complex Environmental Matrices
... The unique ability of the RT-qPCR based method to analyse large number of samples rapidly, with high degree of sensitivity and specificity, makes it a logical choice over other forms of RNA detection methodologies for ...
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THE advent of the polymerase chain reaction in the
... Ruane and Austin first identified 21 snakes from museum collections (primarily the California Academy of Sciences and the Museum of Comparative Zoology at Harvard University), representi[r] ...
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