quantitative proteomics
Quantitative proteomics in lung cancer
... for proteomics are shotgun pro- teomics; however, the complexity of proteomics datasets requires standards to ensure reproducibility and unam- biguous ...geted proteomics by SRM and MRM with ...
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Synovial tissue quantitative proteomics analysis reveals paeoniflorin decreases LIFR and ASPN proteins in experimental rheumatoid arthritis
... Our results confirm the feasibility of using iTRAQ-based quantitative proteomics to identify DEPs. First, 20 identified proteins showed progressive changes with PA treatment. Next, in the 107 biological ...
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Development and standardization of multiplexed antibody microarrays for use in quantitative proteomics
... for quantitative proteomics studies, diagnostic discovery and biomarker-assisted drug devel- opment [reviewed in ...in proteomics studies, and the absence of a "gold standard", there exists ...
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Quantitative proteomics for identifying biomarkers for Rabies
... control. Quantitative proteomic analysis of rabies proteome employing high resolution tandem mass spectrometry coupled to liquid chromatography (LC-MS/MS) led to 59,798 MS/MS spectra from which we identified 402 ...
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Quantitative Proteomics of Cerebrospinal Fluid in Paediatric Pneumococcal Meningitis
... based quantitative proteomics to identify protein changes in cerebrospinal fluid from children with Streptococcus pneumoniae infection, compared with children admitted to hospital with bacterial meningitis ...
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Quantitative proteomics identifies 38 proteins that are differentially expressed in cucumber in response to cucumber green mottle mosaic virus infection
... can result in the suppression of microbial growth via the activation of protein-mediated resistance [17]. For ex- ample, silencing of the host ATP-synthase γ-subunit (AtpC) and Rubisco activase (RCA) genes in N. ...
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Bioinformatics combined with quantitative proteomics analyses and identification of potential biomarkers in cholangiocarcinoma
... CASK, which has been intensively studied in neurons, is deemed to be an important histone protein in cortical networks and may be involved in the establishment and maintenance of synaptic connections [41–44]. The role of ...
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A guide through the computational analysis of isotope-labeled mass spectrometry-based quantitative proteomics data: an application study
... Question 1) Detection of differentially regulated proteins An approach commonly applied to detect differentially regulated proteins is based on the determination of a user-defined threshold in form of a x-fold change in ...
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Screening of differentially expressed proteins from syncytiotrophoblast for severe early onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics
... Recently, high-throughput proteomic technology has been widely applied to screen differentially expressed pro- teins in different tissues of the placenta, plasma, syncytio- trophoblast extracellular vesicles, and ...
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Comparative quantitative proteomics of prochlorococcus ecotypes to a decrease in environmental phosphate concentrations.
... differences in cellular protein expression, as determined by high throughput large scale quantitative proteomic analysis. The only strain to demonstrate a significantly different growth rate under reduced ...
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Identification of lysine acetylome in cervical cancer by label-free quantitative proteomics
... Several acetylome analyses have been performed to understand the function of acetylation proteins in dif- ferent human cancers [16–18]. To the best of our knowl- edge, there are no reports on large scale analyses of ...
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Multi-faceted quantitative proteomics analysis of histone H2B isoforms and their modifications
... The most striking difference between the cell lines was in the levels of H2B1B and H2B1M. Histone H2B1B only differs from other H2B isoforms at two positions with an alanine-to-serine difference and a ...
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Mass spectrometry-based approaches to targeted quantitative proteomics in cardiovascular disease
... Quantification using SILAC labeled proteins has been used in numerous proteomics studies. Rigorous method validation using a full-length stable isotope labeled pro- tein internal standard has been used rarely. The ...
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Label free quantitative proteomics reveals regulation of interferon induced protein with tetratricopeptide repeats 3 (IFIT3) and 5' 3' exoribonuclease 2 (XRN2) during respiratory syncytial virus infection
... protein content was measured by BCA protein assay (Pierce). A total of 1 mg protein of each sample was separated by one-dimensional isoelectric focussing using an offgel fractionater (Agilent) supplemented with pH 4 to 7 ...
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Analysis of the immune response of human dendritic cells to Mycobacterium tuberculosis by quantitative proteomics
... While M. tuberculosis infection of macrophages has been studied extensively, little is known about the mech- anisms that the mycobacterium uses to mediate cell entry into human DCs. It is plausible that many host factors ...
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Quantitative proteomics signature profiling based on network contextualization
... shotgun proteomics produces sparse data that is highly inconsistent between samples, even from the same phenotype ...in proteomics technolo- gies have yielded higher-quality data matrices with more reliable ...
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Quantitative Proteomics Strategies To Explore The Saccharomyces cerevisiae Proteome
... shotgun proteomics approach, in loading all of the peptides in a sample for simultaneous analysis, and therefore displaying a wide range in size and amino acid composition, may mean some peptide exhibit less ...
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Quantitative Proteomics Shows Extensive Remodeling Induced by Nitrogen Limitation in Prochlorococcus marinus SS120
... horizontal gene transfer (4–6), and this genomic diversity has allowed Prochlorococcus to thrive over a broad range of environmental conditions (5, 7–12). Fifty-four Prochlo- rococcus genomes (13), representative of the ...
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Quantitative proteomics for identifying biomarkers for tuberculous meningitis
... in quantitative mass spectrometry have led to identifica- tion and quantitation of biomarkers which serve as indicators of disease progression, prognosis, drug safety and help to elucidate the mechanism of drug ...
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Accessing to the minor proteome of red blood cells through the influence of the nanoparticle surface properties on the corona composition
... and quantitative proteomics approach, we confirmed the impact of the NP surface functionalization and charge on protein capture, both demonstrating the reduction of the sample concentration range on the NPs ...
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