Quantitative Real Time RT-PCR
Original Article mRNA expression level and clinical value of hypoxia inducible factor 1 alpha in patients with myelodysplastic syndromes: a fluorescence quantitative real-time RT-PCR study
... In our study, we collected bone marrow tissue samples from 74 MDS patients and 20 healthy donors to examine HIF-1α mRNA expression level via fluorescence quantitative real-time RT- PCR. ...
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Determination of the exact copy numbers of particular mRNAs in a single cell by quantitative real time RT PCR
... Biosystems, Foster City, CA, USA), 0.2· µ l of 1·mg·ml –1 yeast tRNA (Roche, Basel, Switzerland), 0.05· µ l of 30·U· µ l –1 Prime RNase inhibitor (Eppendorf, Hamburg, Germany), and 6 10 4 copies of salmon gonadotropin ...
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Quantitative real-time RT-PCR of CD24 mRNA in the detection of prostate cancer
... Our aim was to determine whether QRT-PCR-based detec- tion of CD24 will yield data with sensitivity and specifi- city comparable to those in existing reports based entirely on immunohistochemical staining. In this ...
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Original Article TTLL12 expression in ovarian cancer correlates with a poor outcome
... used quantitative real-time RT-PCR and western blot to determine the expressions of TTLL12 in ovarian cancer cells and tissues and also performed immunohistochemical staining to examine ...
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Original Article Differential expressions of BMPR1α, ACTN4α and FABP7 in Hirschsprung disease
... Abstract: Hirschsprung disease (HSCR) is characterized by the absence of intramural ganglion cells in the nerve plex- uses of the distal gut. Recent studies have shown that the bone morphogenetic protein receptor-type IA ...
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Use of FTA Cards To Transport Throat Swabs and Oral Fluid Samples for Molecular Detection and Genotyping of Measles and Rubella Viruses
... transcription-quantitative real-time PCR (RT-qPCR) or the success of genotyping of MeV from samples stored on FTA cards compared to that for TS or oral fluid (OF) samples that were ...
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Classification and risk stratification of invasive breast carcinomas using a real time quantitative RT PCR assay
... The 126 samples used for qRT-PCR were also analyzed by DNA microarray (Agilent Human A1, Agilent Human A2, and custom oligonucleotide). Labeling and hybridization of RNA for microarray analysis were performed ...
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Highly Sensitive Methods Based on Seminested Real Time Reverse Transcription PCR for Quantitation of Human Immunodeficiency Virus Type 1 Unspliced and Multiply Spliced RNA and Proviral DNA
... seminested real-time reverse transcription-PCR (RT-PCR) that combines the accuracy and precision of real-time PCR and the sensitivity of nested ...single-step ...
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Broadly Reactive and Highly Sensitive Assay for Norwalk Like Viruses Based on Real Time Quantitative Reverse Transcription PCR
... Nucleotide sequences of ORF1-ORF2 junction regions from 71 strains. To confirm that it is highly conserved within each genogroup, we sequenced the ORF1-ORF2 junction regions of NLVs isolated from 71 stool specimens ...
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Concurrent Genotyping and Quantitation of Cytomegalovirus gB Genotypes in Solid Organ Transplant Recipients by Use of a Real Time PCR Assay
... a real-time genotyping and quantitative PCR (RT-GQ-PCR) assay to genotype cytomeg- alovirus (CMV) and quantify viral loads simultaneously in solid organ transplant (SOT) ...the ...
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Research Progress on Reference Genes of Insect for Quantitative Real time Reverse Transcription PCR (RT qPCR)
... Abstract Quantitative real-time reverse transcription PCR (RT-qPCR) has become the most important method for the quantification of mRNA transcription levels owing to its, specificity, ...
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Paediatric acute myeloid leukaemia with the t(7;12)(q36;p13) rearrangement: a review of the biological and clinical management aspects
... by real time quantitative PCR (RT-Q-PCR) and did not observe HLXB9 expression in healthy CD34-positive bone mar- row cells, but described increased levels in the bone marrow of ...
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Accurate normalization of real time quantitative RT PCR data by geometric averaging of multiple internal control genes
... Several variables need to be controlled for in gene-expres- sion analysis, such as the amount of starting material, enzy- matic efficiencies, and differences between tissues or cells in overall transcriptional activity. ...
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Superiority of Digital Reverse Transcription PCR (RT PCR) over Real Time RT PCR for Quantitation of Highly Divergent Human Rhinoviruses
... transcription-quantitative PCR (RT-qPCR) and reverse transcription-digital PCR (RT-dPCR) for quantifying HRV RNA using genotype-specific primers and probes and a consensus primer/probe ...
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Hepatitis E Virus (HEV) Detection and Quantification by a Real Time Reverse Transcription PCR Assay Calibrated to the World Health Organization Standard for HEV RNA
... A real-time reverse transcription-quantitative PCR (RT-qPCR) assay utilizing Pleiades probe chemistry and an RNA internal control for the simultaneous detection and quantification of HEV ...
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Characterisation of an efficient atrazine-degrading bacterium, Arthrobacter sp. ZXY-2: an attempt to lay the foundation for potential bioaugmentation applications
... utilised real-time quantitative PCR (RT-qPCR) analysis to measure the transcriptional expression of three atrazine-degrading genes throughout the entire degradation ...
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Immunological assays for chemokine detection in in-vitro culture of CNS cells
... Although RT-PCR is a sensitive tool for assaying mRNA, it is not precise due to the exponential nature of the amplification ...difficulty, real- time, quantitative PCR was ...
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Real-time PCR and real-time RT-PCR applications in food labelling and gene expression studies
... Conventional RT-PCR was widely used for gene-specific mRNA quantification during the early days of the introduction of real-time ...called quantitative endpoint RT-PCR ...
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Establishment and Application of Digital RT PCR Assay for Detection of Avian Influenza Virus H9 Subtype
... digital RT-PCR method for rapid detection of H9 subtype influenza was es- tablished by comparing the two methods of digital RT-PCR and real-time quantitative ...
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Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
... the RT-PCR assay. RT-PCR failed to identify some specimens ...corresponding RT- PCR primers and ...Overall, RT-PCR was relatively easy to perform and more sensitive ...
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