Ralstonia eutropha
Expression of the entire polyhydroxybutyrate operon of Ralstonia eutropha in plants
... PHAs are produced by, and accumulated, in hundreds of species of bacteria, serving for intracellular carbon and energy storage. One of the major forms of PHA is poly-3-hydroxybutyrate (PHB). The biosynthesis of PHB in ...
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Modification of acetoacetyl-CoA reduction step in Ralstonia eutropha for biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) from structurally unrelated compounds
... Results: Ralstonia eutropha strains capable of synthesizing P(3HB-co-3HHx) from not only fructose but also glucose and glycerol were constructed by integrating previously established engineering ...
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Production of Pha (poly hydroxy alkanoates) by Ralstonia eutropha on hydrolysed sago starch as main substrate using fed-batch cultivation method
... The Ralstonia eutropha IAM 12368 was obtained from IAM Culture Collection, Institute of Molecular and Cellular Biosciences, the University of Tokyo, ...
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Examination of PHB Depolymerases in Ralstonia eutropha: Further Elucidation of the Roles of Enzymes in PHB Homeostasis
... manufacturing. Ralstonia eutropha is regarded as the model organism for PHA ...R. eutropha strains. In order to analyze the impact of PhaZs on R. eutropha granule architecture, we performed ...
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Increasing the metabolic capacity of Escherichia coli for hydrogen production through heterologous expression of the Ralstonia eutropha SH operon
... with the introduction and expression of non-native hydro- gen producing pathways including foreign hydrogenases. Moreover, this approach has the potential for increasing other properties of interest including the use of ...
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Simulation and Model Validation of Batch PHB Production Process Using Ralstonia eutropha
... A mathematical model that represents PHB production fermentation is useful for design, optimization and control of the process. A mathematical model can be best developed when the process is well understood. To develop ...
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CDC Group IV c 2: a New RalstoniaSpecies Close to Ralstonia eutropha
... genus Ralstonia, has been isolated in several human ...R. eutropha from CDC group IV c-2, which suggests that CDC group IV c-2 is a new species of the genus ...
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Implications of various phosphoenolpyruvate-carbohydrate phosphotransferase system mutations on glycerol utilization and poly(3-hydroxybutyrate) accumulation in Ralstonia eutropha H16
... The enhanced global biodiesel production is also yielding increased quantities of glycerol as main coproduct. An effective application of glycerol, for example, as low-cost substrate for microbial growth in industrial ...
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Diverse protein regulations on PHA formation in Ralstonia eutropha on short chain organic acids
... study, Ralstonia eutropha was used to elucidate the protein regulations when the bac- terial cells pre-cultivated on glucose were exposed to three representative short chain or- ganic acids, acetic, ...
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Large scale extraction of poly(3-hydroxybutyrate) from Ralstonia eutropha H16 using sodium hypochlorite
... Isolation of polyhydroxyalkanoates (PHAs) from bacterial cell matter is a critical step in order to achieve a profitable production of the polymer. Therefore, an extraction method must lead to a high recovery of a pure ...
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Influence of Matric Potential on Survival and Activity of Genetically Engineered Ralstonia eutropha H850Lr
... lux-marked Ralstonia eutropha H850Lr has been ...[1]. Ralstonia eutropha H850Lr was used in this study because of having two important characteristics appropriate for investigation on the fate ...
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Growth of the facultative chemolithoautotroph Ralstonia eutropha on organic waste materials: growth characteristics, redox regulation and hydrogenase activity
... β-proteobacterium Ralstonia eutropha H16 (Cupriavidus necator) is one of the most studied model organisms for growth on H 2 and CO 2 ...R. eutropha H16 is also a biologically significant bac- terium ...
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Bioenergetic Aspects of Dibenzothiophene Desulfurization by Growing Cells of Ralstonia eutropha
... by Ralstonia eutropha for DBT desulfurization showed that the BDS process was efficiently performed in energy-sufficient culture media, where the carbon source (glucose) was in excess (Dejaloud et ...
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Compositional regulation of poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) by replacement of granule-associated protein in Ralstonia eutropha
... The most abundant PHA in nature is poly(3-hydroxy- butyrate) [P(3HB)], which is generally synthesized from acetyl-CoA through three consecutive reactions by β-ketothiolase (PhaA), NADPH-dependent acetoa- cetyl-CoA ...
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Aerobic degradation of methylene blue from colored effluents by Ralstonia eutropha
... bacterium, Ralstonia eutropha, as an efficient degrader of phenolic compounds, has been reported in previous works (Nickzad et ...R. eutropha toward MB in aqueous media under aerobic ...
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(S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (FadB’) from fatty acid degradation operon of Ralstonia eutropha H16
... In this study (S)-3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA hydratase (H16_A0461/FadB ’ , gene ID: 4247876) from one of two active fatty acid degradation operons of Ralstonia eutropha H16 has been ...
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Development of a broad-host synthetic biology toolbox for ralstonia eutropha and its application to engineering hydrocarbon biofuel production
... R. eutropha H16, comprising six vec- tors spanning three compatibility groups, four promoter systems responding to three chemical inducers, a T7 5’ mRNA stem-loop structure, and three ...
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Recombinant Ralstonia eutropha engineered to utilize xylose and its use for the production of poly(3-hydroxybutyrate) from sunflower stalk hydrolysate solution
... Results: Recombinant R. eutropha NCIMB11599 expressing the E. coli xylAB genes encoding xylose isomerase and xylulokinase respectively, was constructed and examined for the synthesis of poly(3‑hydroxybutyrate) ...
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Use of Statistically-Based Design of Experiment to Study Olive-Oil Mill Wastewater Biodegradation by <i>Ralstonia Eutropha</i> - Volume 4 Number 5 (Oct. 2013) - IJCEA
... R. eutropha was successfully adapted to OMW and the relationship between the adapted bacterium and OMW biodegradation was quantitatively satisfied using an RSM ...R. eutropha performance, ...
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An analysis of the changes in soluble hydrogenase and global gene expression in Cupriavidus necator (Ralstonia eutropha) H16 grown in heterotrophic diauxic batch culture
... Up-regulation of expression of hoxF , hypF2 and hoxA genes in response to growth on glycerol (derepression of the soluble hydrogenase genes).. qRT-PCR was employed to analyse the levels [r] ...
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