Random Amplified Polymorphic DNA PCR
Determination of Genetic Diversity of Four Guppy Strains Using the Random Amplified Polymorphic DNA-PCR
... for DNA isolation, DNA amplification, and electrophoresis results of DNA isolation and ...amplification. DNA purity calculation at the Central Laboratory, Padjadjaran ...
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Utility of Random Amplified Polymorphic DNA PCR and TaqMan Automated Detection in Molecular Identification ofAspergillus fumigatus
... template because of the reporter dye released as the probe was digested by Taq polymerase. Data acquisition and analysis were performed by using the Fluorescence Data Manager 2-Reporter Multicomponent spreadsheet and ...
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Comparison of PCR, Nested PCR, and Random Amplified Polymorphic DNA PCR for Detection and Typing of Ureaplasma urealyticum in Specimens from Pregnant Women
... specimen) PCR (Table ...single-step PCR only after culture of the clinical ...nested PCR, it is still necessary to confirm the presence of ureaplasmas by culture, since a visible amplification ...
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Genetic structure and diversity among three Greek sheep breeds using Random Amplified Polymorphic DNA-PCR
... Genetic structure and diversity of 120 animals from three Greek local breeds were investigated by Random Amplified Polymorphic DNA (RAPD) - PCR method. Sheep samples originated from the[r] ...
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Characterization of Hafnia alvei by biochemical tests, random amplified polymorphic DNA PCR, and partial sequencing of 16S rRNA gene
... Results with the molecular methods indicate that eaeA-pos- itive H. alvei strains have little genetic diversity, in contrast to the other H. alvei strains. The present RAPD-PCR results are in accordance with the ...
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Typing of Clinical and Environmental Aeromonas sp Strains by Random Amplified Polymorphic DNA PCR, Repetitive Extragenic Palindromic PCR, and Enterobacterial Repetitive Intergenic Consensus Sequence PCR
... The DNA profiles of 18 pathogenic strains isolated from stool specimens collected from Polish children, who were hospitalized in one unit of a hospital in Poznan´, Poland, did not form a separate group of related ...
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Use of Random Amplified Polymorphic DNA PCR To Examine Epidemiology of Stenotrophomonas maltophilia andAchromobacter (Alcaligenes)xylosoxidans from Patients with Cystic Fibrosis
... (35). The resulting DNA pellet was resuspended in H 2 O containing RNase and quantified by A 260 . All preparations were frozen at ⫺80°C until use. RAPD typing. The RAPD primer sequences were provided by Eshwar ...
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Identification of Leishmania Species Causing Cutaneous Leishmaniasis Using Random Amplified Polymorphic DNA (RAPD-PCR) in Kharve, Iran
... Background: Leishmaniasis, especially cutaneous leishmaniasis, is considered an important health problem in many parts of Iran including Kharve, Khorasan Razavi province. Cutaneous leishmaniasis is caused by various ...
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Experimental embryonic somatic chimaerism in the sheep confirmed by random amplified polymorphic DNA assay
... for DNA analysis. The random amplified polymorphic DNA-PCR method supplied two markers of chimae- rism, which were amplification products of 643 bp and 615 bp long DNA ...
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PCR based random amplified polymorphic DNA fingerprinting of Yersinia pseudotuberculosis and its practical applications
... Although many common bands appeared among those 10 strains in the RAPD arrays with each primer, most of the primers yielded their own RAPD pattern, unique to each strain (Fig. 2), sugges[r] ...
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Identification of Legionella Species by Random Amplified Polymorphic DNA Profiles
... for DNA extraction, 5 h for the PCR procedure, and 3 h for electrophoresis) but remains practicable for laboratories having all of the Legionella refer- ence ...
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Random amplified polymorphic DNA (RAPD) markers and its applications
... the random amplified polymorphic DNA (RAPD) technique based on the poly- merase chain reaction (PCR) has been one of the most commonly used molecular techniques to develop DNA ...
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Mycobacterium malmoense Specific Nested PCR Based on a Conserved Sequence Detected in Random Amplified Polymorphic DNA Fingerprints
... in random amplified polymorphic DNA (RAPD) fingerprints of 45 ...nested PCR that was ...a PCR product of the expected ...
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An analysis of apricot cultivars by random amplified polymorphic DNA and microsatellite primers
... nuclear DNA was demonstrated by Delseny et ...ideal DNA mark- ers for genetic mapping and population-genetic studies because of their abundance, co-dominant character, ease of detection by PCR, ...
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Genetic Relationship Of Cashew Germplasm Based On Random Amplified Polymorphic Dna
... on Random Amplified Polymorphic ...of DNA by using RAPD ...as: DNA extraction and purification, loading and running of PCR product, RAPD and data ...shown DNA band ...
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Application of random amplified polymorphic DNA analysis to differentiate strains of Salmonella enteritidis
... of PCR-based techniques has had a revolu- tionary impact on the diagnosis of infectious ...microbial DNA or RNA sequences, they have emerged as a highly sensitive and specific method for identifying ...The ...
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A Pseudoautosomal Random Amplified Polymorphic DNA Marker for the Sex Chromosomes of Silene dioica
... The backcross was done under controlled greenhouse con- ditions between one of the “unusual” males (possessing both the male and the female markers) and the original mother. Figure 1.—Segregation of a 590-bp and an ...
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Genetic diversity in clinical isolates of the dimorphic fungus Blastomyces dermatitidis detected by a PCR based random amplified polymorphic DNA assay
... rapid PCR-based assay using arbitrary primers was ...RAPD PCR technique for examining genome diversity and isolate identification for ...of DNA prepared by a boiling-lysis–phenol extraction ...
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Random amplified polymorphic DNA analysis of clinically and environmentally isolated Cryptococcus neoformans in Nagasaki
... RFLP, random amplified polymorphic DNA (RAPD) analysis by an arbitrary primer (AP)-PCR method is simple and quick and does not require exhaustive characterization of the sequence of ...
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Genetic variability of Amorphophallus muelleri Blume in Java based on Random Amplified Polymorphic DNA
... adalah random amplified polymorphic DNA ...penanda DNA yang lain, seperti restriction fragment length polymorphisms (RFLP) dan simple sequence repeats (SSR), teknik RAPD lebih murah, ...
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