Rap1/2
Telomere protein Rap1 is a charge resistant scaffolding protein in chromosomal bouquet formation
... Moreover, Rap1 is highly phosphorylated in mi- totic cells ...between Rap1 and the Bqt1-2 complex, potentially by means of post-translational modifications of one or both interacting partners, may be ...
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Increased sugar uptake promotes oncogenesis via EPAC/RAP1 and O GlcNAc pathways
... inhibitor in basal medium (17.5 mM glucose), they formed orga- nized colonies, but the rate of proliferation remained higher than in S1 acini (Figure 5, A–C). Whereas β1 integrin expression and AKT activity were reduced ...
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RAP1 mediated MEK/ERK pathway defects in Kabuki syndrome
... rescue rap1 morphants (Fig- ure 4, D and E) in a manner similar to the RAP1A mutant allele, while expression of the mutant allele in control embryos was indis- tinguishable from the expression of WT RAP1B mRNA ...
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The Role of EPAC Signaling in Memory Consolidation and Sleep Deprivation
... It is well established that cAMP signaling within neurons plays a major role in the formation of long-term memories. cAMP has three targets, protein kinase A (PKA), hyperpolarization-activated cyclic nucleotide- ...
152
Mesenchymal high grade glioma is maintained by the ID RAP1 axis
... the 2 most robust and alternative categories defined as proneural and mesenchymal and linked to better and worse clinical outcome, respectively (20, 26, ...the 2 key human glioma sub- ...although 2 ...
14
Structural Analysis of Active Site Conformations in Ras and Rap1 GTPases.
... The Ras/Raf/MEK/ERK signaling pathway is the most well studied of five known mitogen activated protein kinase (MAPK) cascades involved in the mediation and timing of signaling events in the cell [74]. This pathway is ...
173
PubMedCentral-PMC4707086.pdf
... the RAP1 regulators, CalDAG-GEFI and RASA3, is critical for both the prevention of premature platelet activation in circulation as well as for hemostatic plug formation at sites of vascular ...Ca 2+ and the ...
12
RASA3 is a critical inhibitor of RAP1 dependent platelet activation
... Euthanized mice were weighed, and tissues were dissected for weighing and/or analysis. Spleen and hind leg tissue from 3 control and mutant mice were fixed in Bouin’s solution (Sigma-Aldrich). Hind legs were left in ...
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Modifying Rap1-signalling by targeting Pde6δ is neuroprotective in models of Alzheimer’s disease
... /v) 2-mercaptoethanol) and were denatured and reduced by incubation at 95 °C for 10 ...incubated 2 h or overnight in tris- buffered saline (TBS, pH ...for 2 h or overnight. After washing and ...
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Repressor and activator protein accelerates hepatic ischemia reperfusion injury by promoting neutrophil inflammatory response
... factor 2-interacting protein 1), is a component of the shelterin complex at the telomere and dependent on TRF2 for stability and recruitment ...complex, Rap1 is dispensable for telomere end capping and does ...
13
Breaking barriers: insight into the pathogenesis of neovascular age-related macular degeneration
... receptor 2 activates the small guanosine triphosphatase (GTPase) of the Rho family, Rac1, in CECs, which in turn facilitates CEC ...suggesting Rap1 activation may be another potential target for preventing ...
10
RasGRP3 regulates the migration of glioma cells via interaction with Arp3
... and Rap1 activity whereas silencing of endogenous RasGRP3 expression in glioma cells that express high levels of this protein decreased Ras but not Rap1 activation, suggesting that RasGRP3 might have ...
15
PubMedCentral-PMC4872870.pdf
... RasGRP family members have four conserved functional domains: i) an N-terminal Ras- exchange motif (REM), ii) a catalytic CDC25 domain, and a C-terminal regulatory region comprised of iii) two EF hands and iv) an ...
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The High-Risk Human Papillomavirus Type 16 E6 Counters the GAP Function of E6TP1 toward Small Rap G Proteins
... cogenic transformation. Thus, identification of novel cellular targets of HPV E6 oncoprotein and elucidation of biochemical activities of these targets are likely to reveal important cell growth regulatory pathways that ...
7
The AF-6 Homolog Canoe Acts as a Rap1 Effector During Dorsal Closure of the Drosophila Embryo
... Figure 2.—Colocalization of Rap1, Cno, and junction mark- ers Arm and ...(a–c); Rap1 protein, but not Cno, is present in the entire basolateral membrane compartment, as well as in the cytoplasm ...
12
Loss of phosphodiesterase 4D mediates acquired triapine resistance via Epac-Rap1-Integrin signaling
... of Rap1 by the prenylation inhibitor zoledronic acid [54, 55] re-sensitized SW480/tria against ...several Rap1 GEFs, including for example C3K (Crk SH3-domain-binding guanine nucleotide- releasing factor, ...
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Apical Accumulation of the Sevenless Receptor Tyrosine Kinase During Drosophila Eye Development Is Promoted by the Small GTPase Rap1
... Eye-antennal imaginal discs from third instar larvae were dissected in plain Schneider medium (Invitrogen), fixed in 13 phosphate-buffered saline (13 PBS); 4% paraformalde- hyde for 15 min at room temperature and washed ...
29
The RNA binding protein Mex3b regulates the spatial organization of the Rap1 pathway
... To explore Mex3b functions in vivo, we generated mice carrying a conditional Mex3b allele with two loxP DNA sequences framing exon 2, which encodes 463 amino acids out of the 576 amino acids of the Mex3b protein ...
12
Rap1 Relocalization Contributes to the Chromatin-Mediated Gene Expression Profile and Pace of Cell Senescence
... (A) Rap1 binds all core histone promoters at ...of Rap1 are sufficient to repress histone gene expression. (C) Rap1 occupancy at core histone promoters is increased in wild type cells carrying the ...
282
Cryptic Virulence and Avirulence Alleles Revealed by Controlled Sexual Recombination in Pea Aphids
... the RAP1 resistance gene, whereas RAP1 was ineffective against N116 (Stewart et ...to 2-day-old nymphs, adult aphids were transferred to new ...
18