real time DNA detection
A Real-time Resistance Measurement for DNA Amplification and Detection
... of DNA amplification and detection efficiency were the composition of LAMP reaction liquid, the resistance electrode of real-time detection, and the method of the results’ ...design, ...
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Molecular Diagnostic Testing for Aspergillus
... Multiplex real-time PCR has also been used to detect azole-resistant strains directly in BAL fluid from patients at risk for IA ...fumigatus DNA, this multiplex assay was shown to achieve a limit of ...
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A new method for detection of pfmdr1 mutations in Plasmodium falciparum DNA using real time PCR
... produced by Applied Biosystems and labelled with reporter dyes, 6FAM or VIC, at their 5' ends and non-fluo- rescent quencher molecules at their 3' ends. Primer and probe concentrations were optimized according to the ...
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A real time PCR screening assay for the universal detection of lumpy skin disease virus DNA
... undertake preventative measures. Therefore, the LSD044 targeting assay presented here serves as an easy screen- ing technique for unambiguous diagnostics of lumpy skin disease. Despite the recombination, the targeted ...
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Evaluation of Transported Dry and Wet Cervical Exfoliated Samples for Detection of Human Papillomavirus Infection
... HPV detection (9, ...HPV detection (2, 7, ...HPV detection and genotyping were determined by dot blot hybridization and the liquid bead microarray assay (LBMA), respectively ...HPV DNA and the ...
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Evaluation of a real time PCR assay for detection and quantification of bacterial DNA directly in blood of preterm neonates with suspected late onset sepsis
... of real-time PCR for diagnosis of neonatal sepsis, and a recent meta-analysis indicates that PCR may be a valuable tool in the diagnostic arsenal [8– ...Multiplex real-time PCR could over- ...
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Comparison between Quantitative Nucleic Acid Sequence Based Amplification, Real Time Reverse Transcriptase PCR, and Real Time PCR for Quantification of Leishmania Parasites
... ferent DNA and RNA amplification methods have been established for detection and quantification of Leishmania par- asites, including quantitative real-time PCR (qPCR) and quan- titative ...
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Detection of Cryptococcus neoformans DNA in Tissue Samples by Nested and Real-Time PCR Assays
... antigen detection, or by culture, but it can be more difficult for other clinical specimens such as bronchoal- veolar lavage fluids, lung biopsies, or blood ...antigen detection is a sensitive and specific ...
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Duplex Real Time SYBR Green PCR Assays for Detection of 17 Species of Food or Waterborne Pathogens in Stools
... template DNA samples from 200 l of these stool suspensions were directly extracted using QIAamp DNA Stool Mini ...duplex real-time LC-PCR was performed within 1 h with 30 glass capillary ...
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Clinical Significance of Quantifying Pneumocystis jirovecii DNA by Using Real Time PCR in Bronchoalveolar Lavage Fluid from Immunocompromised Patients
... and/or pneumonia, the detection of P. jirovecii DNA cannot be regarded as simple carriage. For some authors, cases correspond- ing to positive qPCR and negative microscopy warrant specific therapy when ...
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Increased Detection Rate in Diagnosis of Herpes Simplex Virus Type 2 Meningitis by Real Time PCR Using Cerebrospinal Fluid Samples
... first time) and those with recurrent ...HSV-2 DNA in CSF, detected with nested PCR; (ii) present seroconversion against HSV-2; (iii) con- current herpetic genital lesions and negative differential ...
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Detection and Quantification of Infectious Hypodermal and Hematopoietic Necrosis Virus and White Spot Virus in Shrimp Using Real Time Quantitative PCR and SYBR Green Chemistry
... assay time and allowing the screening of a large number of samples in a relatively short ...the DNA used for the amplification as well as the specificities of the virus-specific primers used for SYBR Green ...
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Evaluation of Real Time and Conventional PCR Targeting Complex 85 Genes for Detection of Mycobacterium leprae DNA in Skin Biopsy Samples from Patients Diagnosed with Leprosy
... genomic DNA to M. leprae DNA, which probably inhibit ...leprae DNA amplification. Potential inhibition by human DNA in ...total DNA extracted from human samples be quantified and ...
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Detection of a Point Mutation Associated with High Level Isoniazid Resistance in Mycobacterium tuberculosis by Using Real Time PCR Technology with 3′ Minor Groove Binder DNA Probes
... extracted DNA, 1 M primers S and AS, and 200 nM wild-type and mutant probes (Applied Biosystems ...and detection were performed using the iCycler iQ Real-Time PCR Detection system ...
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Real Time PCR for Detection and Differentiation of Gram Positive and Gram Negative Bacteria
... ferent biological fluids (water, plasma, urine, sputum), and DNA was extracted. Gram-positive bacteria included Entero- coccus faecalis, Enterococcus faecium, Streptococcus pyogenes, Staphylococcus epidermidis, ...
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Quantitative Detection of Hepatitis B Virus DNA by Real Time Nucleic Acid Sequence Based Amplification with Molecular Beacon Detection
... amplicon detection during the amplification ...HBV DNA real-time NASBA, the time-point at which a reaction reaches the threshold of detection and thus becomes positive, is easily ...
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The Effect Analysis of Different Experimental Methods for the Diagnosis of Invasive Pulmonary Aspergillosis in a Rat Model
... (GM) detection and quantitative real-time PCR assay (qRT-PCR) for the diagnosis of inva- sive pulmonary aspergillosis in a rat ...fumigatus DNA was amplified from 14 of 48 whole blood samples ...
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Development of a Quantitative Real Time Detection Assay for Hepatitis B Virus DNA and Comparison with Two Commercial Assays
... Plasmid DNA was isolated on the Vistra Labstation (Amersham Phar- macia Biotech, Roosendaal, The ...the real- time PCR detection assay, a standard curve of the plasmid ranging from 10 million ...
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Comparison of a New Quantitative ompA Based Real Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays
... involving real-time PCR protocols (16, 23) have shown the overall superiority of this technique over con- ventional ...on real-time technology, preferably in a multicenter comparison trial ...
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Detection of Orthopoxvirus DNA by Real Time PCR and Identification of Variola Virus DNA by Melting Analysis
... the detection of orthopoxvirus genomes and the identification or exclusion of variola ...of detection systems for distinct regions of the orthopoxvi- rus genome for the PCR diagnostics of clinical and ...
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