real-time DNA quantitation
Highly Sensitive Methods Based on Seminested Real Time Reverse Transcription PCR for Quantitation of Human Immunodeficiency Virus Type 1 Unspliced and Multiply Spliced RNA and Proviral DNA
... seminested real-time PCR methods. Since the sensitivity of single-step real-time (RT-)PCR methods was suboptimal for the quantitative detection of HIV-1 RNA and DNA in PBMC from ...
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Real-Time Reverse Transcription-PCR Quantitation of Substance P Receptor (NK-1R) mRNA
... Cloning of NK-1R cDNA fragment. The NK-1R mRNA fragment was cloned and identified with the human NK-1R primer pairs (HSPR3/HSPR4) from IM9 cells as reported earlier (14, 15). Briefly, the PCR products amplified by these ...
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Comparison of Different Methods of Isolation of DNA of Commonly Encountered Candida Species and Its Quantitation by Using a Real Time PCR Based Assay
... of DNA prepared to the listed protocols, sera from patients and from spiked blood were subjected to the isolation procedures according to each protocol, with the exception that the enzyme, the lysis buffer and ...
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Evaluation of Multiplex Type Specific Real Time PCR Assays Using the LightCycler and Joint Biological Agent Identification and Diagnostic System Platforms for Detection and Quantitation of Adult Human Respiratory Adenoviruses
... our real-time PCR assays did not generate false-positive results when challenged with genomic DNA extracted from other agents which cause respiratory disease such as Hae- mophilus influenzae, ...
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Detection of U S , Lelystad, and European Like Porcine Reproductive and Respiratory Syndrome Viruses and Relative Quantitation in Boar Semen and Serum Samples by Real Time PCR
... disrupting agent, such as dithiothreitol or -mercaptoethanol, so it is similar to those used in other differential lysis tech- niques that release only somatic cell DNA and not sperm cell DNA (22, 31, 35, ...
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High Throughput Real Time Reverse Transcription PCR Quantitation of Hepatitis C Virus RNA
... target DNA by agarose gel electrophore- sis. The DNA fragments are then vacuum transferred to Southern blots and are ...from DNA fragments with known copy ...
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Development of a Rapid Real Time PCR Assay for Quantitation of Pneumocystis carinii f sp carinii
... A method for reliable quantification of Pneumocystis carinii in research models of P. carinii pneumonia (PCP) that is more convenient and reproducible than microscopic enumeration of organisms would greatly facilitate ...
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Identification and Quantitation of Cashmere (Pashmina) Fiber and Wool Using Novel Microchip Based Real Time PCR Technology
... based real-time PCR technology offers a very sensitive, specific, and accurate ...traditional real-time PCR systems for being carried as miniature reaction volume in metal ...isolated ...
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Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR
... histologically diagnosed with head and neck cancer, or were at risk for recurrence (previous oral cancer/pre- viously diagnosed). The site of the malignancy is detailed in Table 4. For follow up patients previously ...
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A one step real time RT PCR assay for the quantitation of Wheat yellow mosaic virus(WYMV)
... The development of the TaqMan RT-PCR assays rep- resents a significant advance in nucleic acid quantifica- tion. This approach utilizes the 5′ – 3′ exonuclease activity of Thermus aquaticus (Taq) DNA polymerase to ...
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Development of a Highly Sensitive Genus Specific Quantitative Reverse Transcriptase Real Time PCR Assay for Detection and Quantitation of Plasmodium by Amplifying RNA and DNA of the 18S rRNA Genes
... Quantitative real-time PCR (qPCR) especially has improved the application of PCR be- cause the assay is fast, has very low risk of contamination, and is highly sensitive, specific, and ...
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Real Time Quantitative PCR for Human Herpesvirus 6 DNA
... reference DNA can also be easily identified by virtue of the high interassay reproducibilities of the stan- dard curve ...amplified DNA permits, as in the Southern blot technique, limitation of the ...
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Ultrasensitive quantitation of human papillomavirus type 16 E6 oncogene sequences by nested real time PCR
... The thermal denaturation ("fluorescence extinction") profiles of nested qPCR products generated from pHV101 and SiHa DNA were identical, with a major peak around 85.5°C whose magnitude was proportional to ...
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A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... and real-time PCR ...countries, real-time PCR is the method of choice by providing superior sensitivity, rapid results and low risk of false ...of real-time PCR assays have been ...
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Comparison of real time PCR and hemagglutination assay for quantitation of human polyomavirus JC
... similar DNA T-antigen gene copy numbers from samples containing the same HAU of ...naked DNA. The ratio of empty virions and naked JCV DNA present in virus suspension may be affected by several ...
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Calibrated Real Time PCR Assay for Quantitation of Human Herpesvirus 8 DNA in Biological Fluids
... PCR assays. Optimization of the reactions was achieved by determining the concentrations of magnesium, primers, and probes, as well as the annealing temperature yielding the highest intensity of reporter fluorescent ...
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Development of real time PCR for detection and quantitation of Dengue Viruses
... specific real- time PCR for dengue using TaqMan probes [8,18] or FRET probes ...specific real-time PCR and then serotyped the positive samples by a duplex or a fourplex TaqMan based assay, ...
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Quantitation of Hepatitis B Virus Genomic DNA by Real Time Detection PCR
... accurate quantitation of the serum virus level is useful in monitoring the clinical course of patients with active hepatitis ...HBV DNA levels, as quantified by RTD-PCR, is regarded as ...HBV DNA ...
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Coexistence of Bos taurus and B. indicus Mitochondrial DNAs in Nuclear Transfer-Derived Somatic Cattle Clones
... set Quantitation of mtDNA copy number ratios and hetero- II (E13–E22, ...plasmy: Real-time PCR quantitation was performed using flu- donor cells at passage three (E1 at passage five) and eight ...
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Real Time PCR for Diagnosis of Human Bocavirus Infections and Phylogenetic Analysis
... Following the first description of the hBoV by Allander et al. (1), several reports have confirmed the presence of hBoV infections in patient populations from different continents, suggesting that hBoV is a virus of ...
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