real-time fluorescence PCR
Development of a Real Time Fluorescence PCR Assay for Rapid Detection of the Diphtheria Toxin Gene
... a real-time fluorescence PCR assay for detecting the A and B subunits of diphtheria toxin (tox) ...this real-time PCR assay exhibited 100% sensitivity and ...standard ...
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Detection of Staphylococcus aureus Enterotoxins A to D by Real Time Fluorescence PCR Assay
... rather time-consuming. For this reason, we developed a real- time fluorescence PCR assay (TaqMan PCR) that detects not only the genes for SEA to SED of ...both PCR primers ...
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Sensitive and Specific Method for Rapid Identification of Streptococcus pneumoniae Using Real Time Fluorescence PCR
... of PCR-based testing of clinical isolates. Double blind PCR-based testing results were concor- dant, and spiked negative data verified the absence of PCR inhibition (Table ...the ...
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Rapid Identification of Methicillin ResistantStaphylococcus aureus and Simultaneous Species Confirmation Using Real Time Fluorescence PCR
... Specificity was further confirmed by testing a panel of 40 gram-negative and 40 gram-positive bacterial species, includ- ing methicillin-resistant isolates of Staphylococcus epidermidis (n ⫽ 3), Staphylococcus hominis (n ...
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Real Time Fluorescence PCR Assays for Detection and Characterization of Shiga Toxin, Intimin, and Enterohemolysin Genes from Shiga Toxin Producing Escherichia coli
... genes, PCR assays have been used widely to detect small numbers of STEC present in stool specimens, enrichment broths, or primary fecal cultures (2, 10, 12, 16, 25, 27, 28, 31, ...36). PCR assays have also ...
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Detection, Differentiation, and Quantitation of Pathogenic Leishmania Organisms by a Fluorescence Resonance Energy Transfer Based Real Time PCR Assay
... standard PCR containing Leishmania ...strategy, real-time detection of PCR products was done with the pair of probes chosen above, which enabled exact measurement of yield and ...of ...
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Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
... In this study, there was better correlation between cul- ture and mPCR/RLB than the RT-PCR assay. RT-PCR failed to identify some specimens (S. epidermidis and E. faecalis) that were positive by culture and ...
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Real-time PCR for mrna quantitation
... initial fluorescence to a minimal amount ...single PCR product, a step that is extremely important not only for the LUX primers but also for the Sunrise primers and scorpions because PCR priming and ...
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Use of Fluorescence Resonance Energy Transfer Hybridization Probes To Evaluate Quantitative Real Time PCR for Diagnosis of Ocular Toxoplasmosis
... quantitative real-time PCR revealed intraocular presence of ...positive PCR samples ...quantitative real-time PCR revealed a high parasite number (up to 1,330/l [Table 1, ...
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Quantification strategies in real-time PCR
... strategies, fluorescence chemistries, and instrumentations have led to the development of various assays whereby mRNA transcripts can be quantified precisely in very short ...between real-time RT- ...
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A Parylene Real Time PCR Microdevice
... normal PCR operation, equal ratios of the four standard dNTPs are added to the reaction ...by fluorescence, two properties that are heavily exploited in biochemistry and ...
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Real Time PCR Method for Detection of Zygomycetes
... for fluorescence resonance energy transfer (FRET) probe construction were also supplemented by sequence analysis of PCR products from culture-confirmed zygomycete iso- lates from the Mayo ...
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A sensitive quantification of HHV-6B by real-time PCR
... A PCR can be divided into three ...over time and thereby becomes limiting ...background fluorescence can be converted to the starting concentration of the unknowns from the standard ...
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Frequency of Human Herpesvirus 6 (HHV-6) in Pterygium Using Real-Time PCR Based on SYBR-Green I Fluorescence
... Real-time PCR was performed in an ABI system 7300 with Maxima SYBR Green/ROX qPCR Master Mix (2X) (Ampliqon, Denmark) kit according to the manufacturer’s instructions ...of PCR master mix) to ...
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Comparison of two DNA targets for the diagnosis of Toxoplasmosis by real time PCR using fluorescence resonance energy transfer hybridization probes
... Toxoplasmosis is an infectious disease caused by the pro- tozoan Toxoplasma gondii effecting individuals throughout the world. Two main subpopulations are highly suscepti- ble to this parasite: the fetus and an ...
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Real-time PCR in Food Science: Introduction
... A TaqMan probe is an oligonucleotide double-labelled with a reporter fluorophore at the 5′ end (reporter dye) and with a quencher internally or at the 3′ end (quencher dye). In addition, the probes must be blocked at ...
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Real time PCR applications for diagnosis of leishmaniasis
... the real-time PCR, also named quantitative PCR (qPCR), has become increasingly popu- lar recently since it is fast, has broad dynamic range, and cross-contamination is drastically reduced ...
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Real-Time PCR UNIT 10.3 OVERVIEW AND PRINCIPLES
... excited by a laser at the appropriate wavelength, it absorbs that light and then emits a characteristic wavelength of light. As long as the quencher remains in close proximity it will absorb the emitted light by FRET. ...
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Real time PCR/MCA assay using fluorescence resonance energy transfer for the genotyping of resistance related DHPS 540 mutations in Plasmodium falciparum
... and PCR-RFLP are available for the detection of different mutations but have disadvan- tages such as long processing time and the fact that quan- tification of mixed infections is not possible ...Although ...
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Development and Evaluation of Real Time PCR Based Fluorescence Assays for Detection of Chlamydia pneumoniae
... by PCR and culturing, two of four culture-positive specimens were found positive by the VD2 and VD4 real-time PCR ...and PCR have been reported. In a study to assess the utility of ...
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