Real-time (RT)-PCR MRSA
A new real-time PCR assay for rapid identification of the S. aureus/MRSA strains
... of MRSA detection are labour-intensive and include approximately 3- to 4-day turnaround ...detecting MRSA show diff ering accuracy values (Luteijn et ...alternative time and cost-saving, eff ective ...
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Evaluation of single and double locus real time PCR assays for methicillin resistant Staphylococcus aureus (MRSA) surveillance
... based MRSA detec- tion methods is time constraints which can take 2 to 4 days, during which time carriers can spread ...molecular MRSA detection should contribute to the prevention of ...
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New Real Time PCR Assay for Rapid Detection of Methicillin Resistant Staphylococcus aureus Directly from Specimens Containing a Mixture of Staphylococci
... in MRSA strains, we developed a multiplex PCR assay which combines a primer specific to ...for real-time fluorescence detection of ...the PCR assay, showing that it was specific and did ...
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Multilocus Sequence Typing of Methicillin Resistant Staphylococcus aureus from an Area of Low Endemicity by Real Time PCR
... The isolates analyzed could be assigned to nine clonal com- plexes based on the similarity between sequence types in six of seven loci. CC5 (n ⫽ 7), CC45 (n ⫽ 14), and CC80 (n ⫽ 12) were the predominant clonal complexes ...
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Direct Detection of Staphylococcus Osteoarticular Infections by Use of Xpert MRSA/SA SSTI Real Time PCR
... able to recover bacteria captured in biofilms, as well as bacteria lysed during specimen transportation or because of release of locally delivered antibiotics at the time of prosthesis removal (3, 29, 32, 39). ...
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Evaluation of the LightCycler Methicillin Resistant Staphylococcus aureus (MRSA) Advanced Test for Detection of MRSA Nasal Colonization
... of MRSA infections in health care settings. The LightCycler MRSA Advanced Test (Roche Diagnostics) is a commercially available real-time PCR assay for direct detection of MRSA ...
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Evaluation of the Xpert Methicillin Resistant Staphylococcus aureus (MRSA) Assay Using the GeneXpert Real Time PCR Platform for Rapid Detection of MRSA from Screening Specimens
... of MRSA, especially in critical care areas (1, 5, ...novel real-time PCR assay targeting DNA sequences in the region of the open reading frame orfX, where the staphylococcal cassette ...
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Evaluation of the BD GeneOhm StaphSR Assay for Detection of Methicillin Resistant and Methicillin Susceptible Staphylococcus aureus Isolates from Spiked Positive Blood Culture Bottles
... sa442-mecA real-time PCR method is obvi- ously its higher specificity for the detection of ...of MRSA in mixtures of MSSA and MRCoNS, we did not observe such incorrect identification results ...
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Methicillin Resistant Staphylococcus aureus (MRSA) Detection: Comparison of Two Molecular Methods (IDI MRSA PCR Assay and GenoType MRSA Direct PCR Assay) with Three Selective MRSA Agars (MRSA ID, MRSASelect, and CHROMagar MRSA) for Use with Infection Control Swabs
... the real- time PCR-based IDI-MRSA assay (Infecto Diagnostics, ...GenoType MRSA Direct (Hain Lifescience, Nehren, Germany) ...of MRSA was attempted by inoculation of nonselec- ...
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Current Trends in Rapid Diagnostics for Methicillin Resistant Staphylococcus aureus and Glycopeptide Resistant Enterococcus Species
... multiplex PCR–enzyme-linked immunosorbent assay that detects a species-specific genetic element of ...plus PCR module) also allow the de- tection of mupirocin resistance (ileS; isoleucyl-tRNA syn- thetase ...
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Rapid Identification of Methicillin Resistant Staphylococcus aureus Using Real Time PCR
... quick MRSA screening tests prevent unnecessary procedures as well as the spread of MRSA in case of colonized patients and thus reducing the over- all hospital ...
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Performance of the BD GeneOhm Methicillin Resistant Staphylococcus aureus Test before and during High Volume Clinical Use
... a real-time PCR assay allows procedural optimization in sample preparation, which permits high-volume ...hands-on time, ease of use, and cost, aspects critical to implementation in most ...
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Rapid Screening and Identification of Methicillin Resistant Staphylococcus aureus from Clinical Samples by Selective Broth and Real Time PCR Assay
... of MRSA has increased ...of MRSA strains have spread by clonal dissemination between different hospitals, cities, countries, and even continents and are now the cause of hospital infections worldwide (5, ...
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Clinical Application of Real Time PCR to Screening Critically Ill and Emergency Care Surgical Patients for Methicillin Resistant Staphylococcus aureus: a Quantitative Analytical Study
... of real-time PCR screening assays for methicillin (meticillin)-resistant Staphylococcus aureus (MRSA) colonization is constrained by the predictive values of their results: as MRSA ...
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Multiplex Real Time PCR Assay for Detection of Methicillin Resistant Staphylococcus aureus (MRSA) Strains Suitable in Regions of High MRSA Endemicity
... of MRSA (1–3). Recently, rapid methods for molecular detection of MRSA have been ...single-locus real-time PCR assay that amplifies the staphylococcal cassette chromosome (SCCmec)-open ...
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Exon duplications in the ATP7A gene: Frequency and Transcriptional Behaviour
... For patients whose polymorphic markers were known to be located in the duplicated fragments (from the size of the duplicated fragment or from verification by Q-PCR, see below), these markers were analyzed in order ...
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Comparison and Evaluation of Real Time PCR, Real Time Nucleic Acid Sequence Based Amplification, Conventional PCR, and Serology for Diagnosis of Mycoplasma pneumoniae
... Clinical data. Although the number of positive samples was limited, the clinical data was evaluated as well. Diagnosis for M. pneumoniae was made on the basis of the PCR results. Comparison of clinical data of ...
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Evaluation of a Commercial Real Time PCR Kit for Detection of Dengue Virus in Samples Collected during an Outbreak in Goiânia, Central Brazil, in 2005
... both PCR assays, with a kappa agreement of ...and real-time PCR accounted for at least 90% of the positive results among the 203 confirmed cases (all positive tests) regardless of the day of ...
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Comparison of the BD GeneOhm Methicillin Resistant Staphylococcus aureus (MRSA) PCR Assay to Culture by Use of BBL CHROMagar MRSA for Detection of MRSA in Nasal Surveillance Cultures from Intensive Care Unit Patients
... BDG PCR assay is difficult, since we employed C-MRSA while previous studies utilized other chromogenic media or nonchromogenic selective media (2, 9, 16, 22, 29, 30, ...BDG PCR sensitivity (100%), ...
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Clinical Validation of Multiplex Real Time PCR Assays for Detection of Bacterial Meningitis Pathogens
... Real-time PCR is a powerful tool for detection of human patho- gens because of the high sensitivity and throughput ...Multiplex real-time PCR allows simultaneous detection of ...
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