Real-time TaqMan PCR
Development of a Multitarget Real Time TaqMan PCR Assay for Enhanced Detection of Francisella tularensis in Complex Specimens
... multitarget real-time TaqMan PCR assay capable of rapidly and accurately detecting ...four TaqMan assays demonstrated that three (ISFtu2, 23kDa, and tul4) performed within our ...
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Multilocus Sequence Typing of Mycoplasma hyorhinis Strains Identified by a Real Time TaqMan PCR Assay
... A real-time TaqMan PCR assay based on the gene encoding the protein p37 was developed to detect Mycoplasma hyorhinis. Its speci- ficity was validated with 29 epidemiologically unrelated M. ...
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Rapid and Sensitive Routine Detection of All Members of the Genus Enterovirus in Different Clinical Specimens by Real Time PCR
... using real-time TaqMan quantitative ...enterovirus real-time TaqMan PCR is as sensitive as most of the currently used molecular detection ...the PCR assay can be ...
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Evaluation of Transported Dry and Wet Cervical Exfoliated Samples for Detection of Human Papillomavirus Infection
... by PCR amplification followed by dot blot hybridization ...of PCR products was then dotted onto nylon filters and probed with biotin-labeled -globin and generic HPV probes ...QIAquick PCR ...
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A simplified duplex real time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures
... duplex TaqMan labelled probe-based RT-PCR assay for detection of MG (mgc2 gene) and MS (vlhA gene) reported by Sprygin et ...1. TaqMan duplex real-time PCR show- ing ...
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Evaluation of a Multiplex Real Time Reverse Transcriptase PCR Assay for Detection and Differentiation of Influenza Viruses A and B during the 2001 2002 Influenza Season in Israel
... multiplex real-time TaqMan assay for influenza virus B was not as good (sensitivity, ...multiplex TaqMan assay missed four influenza virus B-positive samples that were positive by culture and ...
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Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... duplex real-time RT- PCR assay was developed to identify H5N6 subtype ...hands-on time. Contrasting to single probe rRT- PCR, this method introduced two different labeled probes so that ...
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TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... the TaqMan duplex real-time RT-PCR presented here uses newly developed prim- ers and probe binding within ORF3 of the avian HEV genome and is suitable for universal ...of ...
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Rapid detection of porcine circovirus type 2 using a TaqMan based real time PCR
... The standard PCV2 plasmid with 10 7 , 10 5 and 10 3 copies was used to evaluate the coefficients of variation (CVs) of the real-time PCR. Intra- and inter-assay CVs for Ct values were both included. ...
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Use of TaqMan Real Time Reverse Transcription PCR for Rapid Detection, Quantification, and Typing of Norovirus
... assay time by moni- toring amplification of target sequences in real time by fluo- rescence resonance energy transfer analysis are widely used for absolute and relative quantification and are a ...
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Development of TaqMan® MGB fluorescent real time PCR assay for the detection of anatid herpesvirus 1
... cycle time and remove of post-amplification process, offering considerable labor savings and allowing higher throughput analysis than conventional PCR assays and thus is favorable for the transition of this ...
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Real Time Quantitative PCR Assays for Detection and Monitoring of Pathogenic Human Viruses in Immunosuppressed Pediatric Patients
... 23 real-time PCR assays based on TaqMan technology has been developed for the detection and monitoring of 16 different viruses and virus families including human polyomaviruses BK virus and JC ...
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Molecular Detection of Linezolid Resistance in Enterococcus faecium and Enterococcus faecalis by Use of 5′ Nuclease Real Time PCR Compared to a Modified Classical Approach
... Taqman PCR. Homozygous linezolid-susceptible and -resis- tant isolates, including E. faecium ATCC 19434 (susceptible), E. faecium 3939 (resistant), E. faecalis V583 (susceptible), and E. faecalis 3696 ...
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Evaluation of Polymerase Chain Reaction and Cobas TaqMan Real Time PCR in the Diagnosis of Tuberculosis: Indian Prospective
... the PCR positivity of IS6110 and MPB64 gene targets was found to be ...the PCR positivity was observed 100% in smear positive, culture positive Line probe assay tested in MDR PTB cases (true positive ...
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Quantification of Human Immunodeficiency Virus Type 1 Proviral Load by a TaqMan Real Time PCR Assay
... TaqMan PCR target region of the specimen from this patient showed a mutation in the primer P1 target region and, more importantly, a second mutation that would prevent hybridiza- tion of the 5⬘ end of the ...
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Original Article The impact of storage time on HCV RNA quantitative detection in serum samples by TaqMan real-time PCR
... HCV PCR, competitive reverse transcription PCR (cRT-PCR), and branched DNA signal amplification assay; However, the effects of those storage conditions on HCV RNA load in serum samples using ...
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A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... nested PCR and/or sequencing [2,22], LAMP [23], and real-time PCR ...countries, real-time PCR is the method of choice by providing superior sensitivity, rapid results and ...
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Comparison of the Xpert MTB/RIF Test with an IS6110 TaqMan Real Time PCR Assay for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens
... of PCR technologies has been achieved with the development of real-time PCR testing ...of real-time PCR are a shortened turnaround time; automation of the ...
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Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load
... transcriptase PCR assay (3), has been widely used in research as well as in clinical ...the PCR, resulting in a dynamic range of 500 to 500,000 ...The real-time PCR technology already ...
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Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
... in PCR mixes at ten different ...Nested PCR and a TaqMan nested real time PCR assay, targeting the 18S rRNA gene, was used to detect ...to PCR and TaqMan PCR ...
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