Recombinant plasmid
Co-delivery of doxorubicin and recombinant plasmid pHSP70-Plk1-shRNA by bacterial magnetosomes for osteosarcoma therapy
... MTB AMB-1 was a generous gift from Dr Weidong Pan (Beijing Key Laboratory of Bioelectromagnetics, Institute of Electrical Engineering, Chinese Academy of Sciences, Peo- ple’s Republic of China). The Chinese Academy of ...
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Cloning and Expression of Recombinant Plasmid Containing P36/LACK Gene of Leishmania infantum Iranian Strain.
... vector. Recombinant plasmid was extracted and analyzed by sequencing, restriction digestion analysis and PCR reac- ...LACK recombinant plasmid was purified from transformed ...
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Global Stewardship of Recombinant Plasmid Sequences
... Recombinant laboratory plasmids (RLPs) are common in biological research and freely shared among academic research laboratories (ARLs), a practice required by many research funding agencies. However, the ...
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Construction and Identification of a Recombinant Plasmid Encoding Echinococcus granulosus Oncosphere Antigen (EG95)Abstract Background: Cystic echinococcosis (CE), as a zoonotic disease cause to health threat and economic losses. Despite implemented cont
... The recombinant EG95 protein vaccine has now been demonstrated effective in conducted vaccine trials in Australia, Argen- tina and China (7-9, ...binant plasmid as a vaccine in the current ...
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Induction of a robust immune response against avian influenza virus following transdermal inoculation with H5-DNA vaccine formulated in modified dendrimer-based delivery system in mouse model
... of recombinant plasmid deoxyribonucleic acid (DNA), pBud-H5-green fluorescent protein (GFP)-interferon-regulatory factor (IRF)3 following delivery using polyamidoamine (PAMAM) dendrimer and transactiva- tor ...
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Construction and Sequencing of Dense Granular14 (GRA14) Gene of Toxoplasma gondii (RH) in Expression Prokaryotic Plasmid PET32a: A Preliminary Study in Vaccine Production
... The recombinant plasmid was analyzed by restriction enzyme and rusconis also the resulting fragments were compared with molecular weight ...pTG19-GRA14 recombinant plasmids following restriction ...
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Evaluation of immunogenicity elicited from two DNA vaccine candidates that expresses the prM and E genes of the dengue 3 virus
... a recombinant plasmid expressing only the E protein of dengue virus 2, containing 94% of the E gene, no response with anti-dengue antibodies, cellular proliferation, or synthesis of cytokines by their ...
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The construction of the eukaryotic expression plasmid pcDNA3.1/azurin and the increased apoptosis of U2OS cells transfected with it
... expression plasmid containing the azurin gene with an influenza virus haemagglutinin 9 peptide HA epitope tag, and transfected the recombinant plasmid ...the recombinant plasmid ...
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Expression of the cloned bacteriophage phi X174 A* gene in Escherichia coli inhibits DNA replication and cell division.
... Upon induction of expression, DNA synthesis in Escherichia coli carrying the recombinant plasmid is severely inhibited; however, these same cells permit 1-galactosidase induction at a ra[r] ...
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<p>Reversal of <em>mcr-1</em>-Mediated Colistin Resistance in <em>Escherichia coli</em> by CRISPR-Cas9 System</p>
... number plasmid pUC19-mcr-1 and recombinant plasmid pCas9-m1 or pCas9-m2, which contain 20 nt or 30 nt sgRNA sequences targeted to mcr-1, ...number plasmid (pUC19-mcr-1) to natural resistance ...
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Complete nucleotide sequence of infectious Coxsackievirus B3 cDNA: two initial 5' uridine residues are regained during plus-strand RNA synthesis.
... The 5' termini of the transfection-derived viruses and that of parental CVB3 were found to be identical, demonstrating that recombinant plasmid DNA, as well as in vitro-synthesized RNA t[r] ...
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Characterization of Wild-Type Adeno-Associated Virus Type 2-Like Particles Generated during Recombinant Viral Vector Production and Strategies for Their Elimination
... pSub201-pAAV/Ad plasmid cotransfection system was developed to eliminate homologous recombina- tion which leads to generation of the wild-type (wt) adeno-associated virus type 2 (AAV) during recombinant ...
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Cloning and expression of cpxA gene from Pectobacterium carotovorum subsp carotovorum
... The recombinant plasmid (pET-15b/cpxA) was transformed into BL21 (DE3) and was induced by different kinds of concentrations (0, 0.001, 0.005, 0.01, 0.05, 0.1, 0.2, 0.5, 1.0 mM) of IPTG. The protein ...
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Simple repeat sequence in Epstein-Barr virus DNA is transcribed in latent and productive infections.
... DNAs from left to right recombinant plasmid pDK225 containing the B95-8 BamHI K fragment, B95-8 virus, a recombinant cosmid containing W91 EcoRI fragment B, W91 virus, P3HR-1 virus, AG87[r] ...
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Cloning and expression of the constant region of rainbow trout (Onchorhynchus mykiss) µ immunoglobulin chain in Escherichia coli
... amplification of a 972bp DNA segment was expected. Electrophoresis of PCR product on 1.5% agarose gel beside a 1kb size marker (Fermentas, Lithuania) showed that PCR has successfully led to amplification of a DNA band of ...
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Efficient production of extracellular pullulanase in Bacillus subtilis ATCC6051 using the host strain construction and promoter optimization expression system
... knockout plasmid solution (100 ng/μL), maintained in 900 μL recovery medium (LB medium added with 1% sorbitol) at 30 °C and 100 rpm for 4–6 ...and plasmid extraction ...
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Molecular characterisation of PHA synthase and the in vivo synthesis of functionalised PHA beads with surface immobilised proteins : a thesis presented in partial fulfilment of the requirements of the degree of Master of Science in Microbiology at Massey University, Palmerston North, New Zealand
... cloning of these plasmids are described below, employing the pET expression system. The pET expression system allows for high overexpression of recombinant genes, resulting in the overproduction of protein. These ...
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Bench-scale Overproduction and Purification of recombinant GCSF in Escherichia coli fed-batch process
... Fermentation process scale up can change the hydrodynamic conditions of recombinant E. coli cultivation bioreactor and reduce productivity in the same operating conditions that have been developed at lab scale. In ...
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Designing a recombinant Bacmid construct of HCV core+1 in Baculovirus expression system
... (9). Recombinant Baculovirus expression systems are one of the important tools for highly efficient gene expression through infection of insect cells (16, ...lian recombinant proteins with high ...
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Generation of mini-Tn7 transposone by cloning of CPV-VP2 into eukaryotic expression cassette, in E. coli
... A number of pFastBac vectors are available for use with the Bac-to-Bac baculovirus expression system: 1), pFastBac1 that has strong AcMNPVpolyhedrin (PH) promoter for high level protein expression and large multiple ...
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