Removal of repeated sequences from hybridization probes
Design and Syntheses of Novel Quenchers for Fluorescent Hybridization Probes
... peptide from the resin was observed and therefore reducing the yield of the desired ...for removal of Z group, that highlighted the need for new methodology to avoid such ...
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Identification of vaccine related polioviruses by hybridization with specific RNA probes
... RNA probes for the identification of poliovirus isolates by blot ...strain-specific probes were ...region sequences was used to estimate the quantities of poliovirus (or enterovirus) RNA in the ...
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Purification of Nucleic Acids by Hybridization to Affinity Tagged PNA Probes
... by hybridization” approach ought to be applicable to the purification of other classes of nucleic acids and specific target molecules, it does not appear from the literature to be widely ...capture ...
7
Specific hybridization probes demonstrate fewer xenotropic than mink cell focus-forming murine leukemia virus env-related sequences in DNAs from inbred laboratory mice.
... We have derived hybridization probes from analogous 100-base-pair segments located within the N-terminal region of gp7O coding sequences which differentiate xenotropic from mink cell foc[r] ...
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Optimizing comparative genomic hybridization probes for genotyping and SNP detection in Plasmodium falciparum
... of probes to examine individual parameters and often hybridize arti ficial samples such as PCR products [26 –30] ...results from the current study were derived from more then 300,000 probes ...
8
Genotype specific RNA probes for direct identification of wild polioviruses by blot hybridization
... genotype-specific probes must be designed to balance geno- type specificity with the capacity to accommodate extensive sequence variation within ...derived from lineages that are most likely to continue to ...
7
Rapid Identification of Staphylococcus aureus Directly from Blood Cultures by Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes
... aureus that previously published DNA probes target (5, 11); however, the PNA probe is shorter than those DNA probes due to the higher affinity of the PNA probe. Initially, the specificity of the PNA probe ...
5
Fluorescence In Situ Hybridization with Peptide Nucleic Acid Probes for Rapid Identification of Candida albicans Directly from Blood Culture Bottles
... target sequences, according to the BLAST search, differed from the probe target sequence by a single ...rRNA sequences for NRRL Y-12983 and NRRL Y-81, but it did not result in a brighter ...
5
Secondary Structure and Hybridization Accessibility of Hepatitis C Virus 3′-Terminal Sequences
... was repeated with the HCV positive- strand 3⬘ terminus as a substrate ...derived from MMLV-mediated ex- tension of primer 3CN are shown in ...cDNAs from all ...
12
Identification of pathogenic Leptospira genospecies by continuous monitoring of fluorogenic hybridization probes during rapid cycle PCR
... Partial sequences of 23S rRNA gene PCR products from 23 strains of 6 pathogenic Leptospira genospecies and from 8 strains of the saprophytic Leptospira biflexa were ...adjacent hybridization ...
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Use of Discriminatory Probes for Strain Typing of Formalin Fixed, Rabies Virus Infected Tissues by In Situ Hybridization
... these probes was initially per- formed by Southern and slot blotting ...stringent hybridization conditions, obtained by use of a high formamide concentration, a high temperature, and a low salt ...
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Repeated DNA sequences from the bovine Y chromosome
... chromosomes, which is a general feature of mammalian Y chromosomes and has lea d to the presence of Y- specific repeats in most species, and the very stable chromosomal [r] ...
230
Research on the Repeated Sequences among tRNA Sequences
... fragment sequences in the earliest stage, later the few fragment sequences amplified after replication, and then tRNA sequences didn’t form a stable structure and stopped to amplify until up to their ...
7
Repetitive DNA sequences as probes for Mycobacterium tuberculosis
... Whole chromosomal DNA probes for rapid identification of Mycobac- terium tuberculosis and Mycobacterium avium complex. Enzymatic amplification of B-globin genomic sequence and restrictio[r] ...
6
Persistence of repeated sequences that evolve by replication slippage.
... NIGON, 1985 Evolution of the primate beta-globin gene region: High rate of variation in CpG dinu- cleotides and in short repeated sequences between man and chimpanzee. N[r] ...
8
Novocastra Fluorescein-conjugated probes for in situ hybridization ISH Detection Kit
... fluorescein-conjugated probes. Sufficient reagents for the in situ hybridization staining of 100 preparations are ...The hybridization is followed by a wash with Tris-buffered ...
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Identification of Borrelia burgdorferi and B hermsii using DNA hybridization probes
... demonstrated that probe 49A hybridized only to the 49-kb linear plasmid (data not shown). This probe hybridized with plasmid-enriched DNA of B. buirgJdoijeri but not with the other six B[r] ...
5
Removal of specular reflections from image sequences using feature correspondences
... scene from inspection footage of pipes, gas tubes, train tracks and concrete ...frames from video footage to reduce specularity from each ...free from specular highlights provided there is at ...
14
THE EVOLUTION OF RESTRICTED RECOMBINATION AND THE ACCUMULATION OF REPEATED DNA SEQUENCES
... Restricted crossing over: In the second section of this paper, we have pro- posed that the reduction in the rate of crossing over near centromeres and telomeres [r] ...
16
De novo methylation of repeated sequences in Coprinus cinereus.
... cinereus (ROSSIGNOL and PICARD 1991 ; RHOUNIM, ROSSIGNOL and FAUGERON 1992): only repeated sequences are methylated, methylation occurs during the dikaryotic stage, no C [r] ...
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