Replication derived from plasmid DNA transfection
Replication of plasmid-derived human papillomavirus type 11 DNA in cultured keratinocytes.
... Plasmid-derived human papillomavirus type 11 (HPV11) DNA has been shown to replicate episomally and semiconservatively following transfection of primary human foreskin keratinocytes.. HP[r] ...
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Infectious alphavirus production from a simple plasmid transfection
... produced from our system can infect multiple cell types in culture and maintain reporter gene ...viral replication or antiviral ...stable plasmid DNA may open up the possibility of using these ...
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Replication, Integration, and Packaging of Plasmid DNA following Cotransfection with Baculovirus Viral DNA
... this plasmid DNA replication is unknown, al- though it has been speculated that it may result from the acquisition of hr sequences following cotransfection ...of plasmid ...
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Mechanism of enhancement of DNA expression consequent to cointernalization of a replication-deficient adenovirus and unmodified plasmid DNA.
... Chemical composition of empty capsids. The density of the empty capsids was determined after collecting the band from the second gradient and directly weighing 1 ml of the band in CsCl. [r] ...
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Unusual amplification in polymerase chain reaction for a plasmid containing an insert derived from bovine genomic DNA
... proteins from interaction with tannins, and modulation of bacterial colo- nization on the tooth ...genomic DNA in bovine tooth germ mRNA encoding the proline- rich protein ...nomic DNA is present in ...
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Plasmid-like replicative intermediates of the Epstein-Barr virus lytic origin of DNA replication.
... viral DNA replication are found as large concate- meric molecules in the infected ...viral DNA replication that yield these concatemers ...of DNA replication, replicative ...
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Replication of hepatitis delta virus RNA in mice after intramuscular injection of plasmid DNA.
... RNA after intramuscular injection of cDNA into mice will allow specific alterations of the HDV genome to be made at the cDNA level and the resulting phenotypic effects to be easily determined in vivo. Our data in this ...
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Transfection with thymidine kinase permits bromodeoxyuridine labelling of DNA replication in the human malaria parasite Plasmodium falciparum
... transferred from cell cycle studies in model ...study DNA repli- cation and the cell cycle in human cells is the labelling of newly-replicated DNA with the modified nucleotide bromo- deoxyuridine ...
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GRS Plasmid Purification Kit Transfection Grade GK (2 MaxiPreps)
... GRS Plasmid Purification Kit – Transfection Grade provides an easy and efficient method for the purification of high quality plasmid DNA from cultured bacterial ...Eluted DNA is ...
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Initiation of baculovirus DNA replication: early promoter regions can function as infection-dependent replicating sequences in a plasmid-based replication assay.
... 1996 From the results of transient plasmid-based replication assays, it has been postulated that homologous regions (hrs) of Autographa californica nuclear polyhedrosis virus (AcMNPV) function as ...
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High-Frequency Intermolecular Homologous Recombination during Herpes Simplex Virus-Mediated Plasmid DNA Replication
... random integration into the host genome. Repair of the mu- tated endogenous gene through homologous recombination would avoid these problems. The efficiency of current gene targeting approaches, however, is generally ...
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The fastest spin-column based procedure for purifying up to 10 mg of ultra-pure endotoxin-free transfection-grade plasmid DNA.
... 600 ml Reservoir 5 Room Temp. ZymoPURE ™ Giga Filter 5 Room Temp. Instruction Manual 1 - Note - Integrity of kit components is guaranteed for up to one year from date of purchase. Reagents are routinely tested on ...
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Constructing and Characterizing a Recombinant DNA Plasmid
... desired DNA frag- ment or gene sequence can be identified, excised from the chromosome, isolated, and cloned into a number of different plasmids or ...The plasmid carrying the gene of in- terest can ...
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Cloning, expression, purification and characterization of replication protein from plasmid pGP2 from Acetobacter estunensis
... Protein; DNA-Binding Activity; ATPase Activity; Phosphatase Activity; Unwinding Activity The replication of eukaryotic and prokaryotic chromo- somes, bacteriophages and bacterial plasmids DNA in- ...
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Gene expression profiles in primary duodenal chick cells following transfection with avian influenza virus H5 DNA plasmid encapsulated in silver nanoparticles
... condensed DNA vector complex with a slightly positive charge could interact electrostatically with the cell membrane and thereby be ...the DNA from degradation. The high cationic charge mediates both ...
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Inhibition of Human Papillomavirus DNA Replication by an E1-Derived p80/UAF1-Binding Peptide
... their DNA replication defects correlate with their capacity to interact with ...for DNA replication than the VI or VE mutant pro- teins, which both retain weak binding to ...viral DNA ...
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Interaction of the Escherichia coli replication terminator protein (Tus) with DNA: a model derived from DNA binding studies of mutant proteins by surface plasmon resonance
... the DNA-binding activity of a ...different DNA molecules simultaneously, or alternatively, the same DNA molecule can be examined with different surface loadings to improve the quality of the data and ...
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DNA transfection screening from single beads
... for transfection activity at the single-bead level has been developed and allowed members of the library to be identified that have the potential to be better than many currently available transfection ...
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Plasmid models for bacteriophage T4 DNA replication: requirements for fork proteins.
... Recombi- nation-dependent plasmid replication also strictly required the T4 single-stranded DNA-binding protein (gene product 32 [gp321), and replication of origin-containing plasmids wa[r] ...
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Plasmid origin of replication of herpesvirus papio: DNA sequence and enhancer function.
... The ori-P of each virus has the general structure of an array of monomer units, followed by an intervening sequence of variable length, followed by four or five partial copies of the mon[r] ...
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