Restriction endonuclease digestion
Differentiation of Acanthamoeba strains from infected corneas and the environment by using restriction endonuclease digestion of whole cell DNA
... Restriction endonuclease digestion of Acanthamoeba whole-cell DNA was used to study the relationship between 33 morphologically identical strains from keratitis cases 30 strains, contact[r] ...
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Chromosomal DNA restriction endonuclease digestion patterns of beta lactamase producing Enterococcus faecalis isolates collected from a single hospital over a 7 year period
... common restriction pattern depicted here has been observed before and designated the MA pattern ...MA restriction endonuclease digestion pattern occurred in fragments of var- ious sizes; for ...
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Comparison of JC and BK Human Papovaviruses with Simian Virus 40: Restriction Endonuclease Digestion and Gel Electrophoresis of Resultant Fragments
... 614-622 Copyright © 1974 American Society for Microbiology Comparison of JC and BK Human Papovaviruses with Simian Virus 40: Restriction Endonuclease Digestion and Gel Electrophoresis of[r] ...
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COMPARISON OF THE RESTRICTION ENDONUCLEASE DIGESTION PATTERNS OF MITOCHONDRIAL DNA FROM NORMAL AND MALE STERILE CYTOPLASMS OF ZEA MAYS L
... High resolution gel electrophoresis has allowed the assignment of fragment number and molecular weight to EcoRI, Sal1 and PstI restriction fragments of mitochondrial DNA from[r] ...
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Endonuclease-Mediated Long PCR and Its Application to Restriction Mapping
... DNA restriction endonuclease ...simplify restriction mapping, a technique central to DNA ...performing restriction digestion prior to PCR amplification to “selectively suppress” the ...
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Characterization of an infective molecular clone of the B-tropic, ecotropic BL/Ka(B) murine retrovirus genome.
... Proviral DNA derived from the progeny virus of the transfected SC-1 cells was analyzed by restriction endonuclease digestion.. This DNA was digested in separate reactions with KpnI,..[r] ...
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Comparison of neutralization and DNA restriction enzyme methods for typing clinical isolates of human adenovirus
... We conclude that typing clinical isolates of adenovirus by restriction endonuclease digestion of viral DNA can be done rapidly, provides additional epidemiological and typing information[r] ...
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Use of restriction fragment length polymorphisms resolved by pulsed field gel electrophoresis for subspecies identification of mycobacteria in the Mycobacterium avium complex and for isolation of DNA probes
... Mycobacterial strains from the Mycobacterium avium complex were compared with each other and with Mycobacterium phlei isolates by restriction endonuclease digestion of chromosomal DNA wi[r] ...
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Characterization of human papovavirus BK DNA.
... Comparison of JC and BK human papovaviruses with simian virus 40: restriction endonuclease digestion and gel electrophoresis of resultant fragments.. Cultivation of papova-like virus fro[r] ...
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Molecular analysis of multiple isolates of the major serotypes of group B streptococci
... Among 10 serotype I isolates, restriction endonuclease digestion followed by conventional AGE revealed five different REA patterns with HindIII and six with HaeIII.. PFGE showed seven RE[r] ...
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In vivo and in vitro analyses of recombinant baculoviruses lacking a functional cg30 gene.
... Furthermore, restriction endonuclease digestion of AcMNPV DNA with SfiI which inactivates cg3O and another AcMNPV gene, pl43, does not affect transient expression from the etl, vp39, or [r] ...
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Comparison of genomic DNAs of different enterococcal isolates using restriction endonucleases with infrequent recognition sites
... In this study, we have compared chromosomal restriction endonuclease digestion patterns of 27 isolates of Enterococcus faecalis from three different locations by using pulsed-field elect[r] ...
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Herpesvirus saimiri strain variability.
... Analysis of size and number of herpesvirus DNA fragments generated by restriction endonuclease digestion has been used by others to distinguish different strains of herpes simplex virus [r] ...
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Construction of Double Gene Expression Vector
... of restriction endonucleases which demand the expression of restriction endonuclease and methylase ...by restriction digestion and ligation (Section ...
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Restriction endonuclease analysis as a taxonomic tool in the study of pig isolates belonging to the Australis serogroup of Leptospira interrogans
... Restriction endonuclease analysis was performed on DNAs from the type strains of the Australis serogroup of Leptospira interrogans by using 20 restriction enzymes, and the electrophoreti[r] ...
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Hemolytic disease of the newborn caused by a new deletion of the entire beta globin cluster
... Initial restriction endonuclease analysis of the DNA showed no grossly abnormal patterns, but studies of polymorphic restriction sites and gene dosage revealed an extensive deletion that removed all ...
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MOLECULAR ANALYSIS OF X-RAY-INDUCED ALCOHOL DEHYDROGENASE (Adh) NULL MUTATIONS IN DROSOPHILA MELANOGASTER
... Restriction endonuclease and Southern blot analysis showed that three of the mutants were normal compared t o the wild-type restriction pattern, with one of the thre[r] ...
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In Vivo Methylation of Bacteriophage φX174 DNA
... In vivo methylation by Escherichia coli K-12 mec+ deoxyribonucleic acid-cytosine methylase protects against in vitro cleavage by the RII restriction endonuclease R-EcoRII.. Endonuclease [r] ...
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DNA amplification and restriction endonuclease analysis for differentiation of 12 species and taxa of Nocardia, including recognition of four new taxa within the Nocardia asteroides complex
... for restriction fragment length polymorphism (RFLP) by using an amplified 439-bp segment of the 65-kDa heat shock protein ...30 restriction endonucleases, digestion with MspI and then ...
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Evaluation of Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Analysis and Spoligotyping for Genotyping of Mycobacterium bovis Isolates and a Comparison with Restriction Fragment Length Polymorphism Typing
... include restriction endonuclease analysis (REA), restriction fragment length polymorphism (RFLP) analysis, spoligotyping, and, more recently, mycobacterial interspersed ...
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