restriction fragment length polymorphism analysis
Development and Application of a New Scheme for Typing Campylobacter jejuni and Campylobacter coli by PCR Based Restriction Fragment Length Polymorphism Analysis
... A molecular typing approach for Campylobacter jejuni and Campylobacter coli was developed with restriction fragment length polymorphism analysis of a 9.6-kb PCR-amplified portion of the ...
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Rapid identification of mycobacteria to species level by PCR restriction fragment length polymorphism analysis of the hsp65 gene and proposition of an algorithm to differentiate 34 mycobacterial species
... PCR-restriction fragment length polymorphism analysis (PRA) (26) of the hsp65 gene present in all mycobacteria (9, 20, 21) offers an easy, rapid, and inexpensive procedure to identify ...
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Restriction Fragment Length Polymorphism Analysis of Some Flagellin Genes of Salmonella enterica
... partial restriction occurred with the en- zymes used, summation of fragment lengths for each profile was ...flagellin fragment lengths varied in accordance with the profiles studied and was often ...
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Differentiation of Burkholderia Species by PCR Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene and Application to Cystic Fibrosis Isolates
... Burkholderia cepacia, first described as the agent of the on- ion soft rot (2), is an opportunistic human pathogen which may be responsible for various nosocomial infections and is espe- cially problematic in patients ...
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Genetic Diversity of Mycobacterium africanum Clinical Isolates Based on IS6110 Restriction Fragment Length Polymorphism Analysis, Spoligotyping, and Variable Number of Tandem DNA Repeats
... Significance of M. africanum genotyping in a probable evo- lutionary scenario for the M. tuberculosis complex. The results obtained demonstrate that phenotypic and/or genotypic tests specifically developed to diagnose M. ...
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Restriction fragment length polymorphism analysis of clinical isolates of Mycobacterium haemophilum
... Among New York patients, the type 2 clone was isolated from skin 11 cases, bone 1 case, blood 1 case, lung 2 cases, and joint 1 case, and 9 of 15 patients with the type 2 clone had AIDS.[r] ...
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Single Nucleotide Polymorphisms in the IS900 Sequence of Mycobacterium avium subsp paratuberculosis Are Strain Type Specific
... as restriction fragment length polymorphism analysis with hybridization to IS900 (19), pulsed-field gel electrophoresis (PFGE) (11, 29), PCR-restriction enzyme analysis ...
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Extensive DNA sequence conservation throughout the Mycobacterium tuberculosis complex
... Differentiation of slowly growing Mycobacterium species including Mycobactenium tuberculosis, by gene amplification and restriction fragment length polymorphism analysis.. Identification[r] ...
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Examining Complex Microbial Communities Using the Terminal Restriction Fragment Length Polymorphism Method and Dedicated TRFLP Analysis Software
... Terminal Restriction Fragment Length Polymorphism analysis could be effectively used to examine microbial communities associated with swine waste lagoon systems (Devine, Chapter ...
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Evaluation of PCR Restriction Profile Analysis and IS2404 Restriction Fragment Length Polymorphism and Amplified Fragment Length Polymorphism Fingerprinting for Identification and Typing of Mycobacterium ulcerans andM marinum
... The identification of mycobacterial species constitutes a crit- ical step in patient management because the results obtained influence the choice of appropriate treatment. Classical pro- cedures to establish the species ...
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Evaluation of Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Analysis and Spoligotyping for Genotyping of Mycobacterium bovis Isolates and a Comparison with Restriction Fragment Length Polymorphism Typing
... include restriction endonuclease analysis (REA), restriction fragment length polymorphism (RFLP) analysis, spoligotyping, and, more recently, mycobacterial interspersed ...
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Comparative Evaluation of Ligation Mediated PCR and Spoligotyping as Screening Methods for Genotyping ofMycobacterium tuberculosis Strains
... on restriction fragment length polymorphism (RFLP) analysis with IS6110 (IS6110 RFLP analysis) as a “gold standard,” we performed a comparative evaluation of spoligotyping and ...
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Molecular Typing of Nocardia Species
... (PCR restriction enzyme pattern analysis), was one of the first molecular techniques to be used for the characterization and identification of Nocardia ...specific restriction endonucleases for each ...
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Low Genetic Variation Suggest Single Stock of Kawakawa Euthynnus affinis (Cantor, 1849) along the Indian Coast
... study, restriction fragment length polymorphism (RFLP) analysis of the mitochondrial D-loop region was employed to examine the levels of genetic diversity among kawakawa samples ...
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Molecular Fingerprinting of Mycobacterium tuberculosisand Risk Factors for Tuberculosis Transmission in Paris, France, and Surrounding Area
... A restriction fragment length polymorphism (RFLP) analysis was performed with clinical isolates of Mycobac- terium tuberculosis isolated during 1995 in 10 hospitals in Paris and ...
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Fast Ligation Mediated PCR, a Fast and Reliable Method for IS6110 Based Typing of Mycobacterium tuberculosis Complex
... IS6110 restriction fragment length polymorphism (RFLP) analysis is the most widely applied method for strain differentiation of Mycobacterium tuberculosis ...allows analysis of ...
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Identification and Enumeration Method of Both Eukaryotic and Prokaryotic Microorganisms in Food Sample
... enzyme restriction fragment poly- morphism analysis (MERFLP), by which bacteria was systematically affiliated [1], and the method to provide numbers of each taxonomically different bacterial groups ...
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Characterization of Lactuca spp germplasm by protein and molecular markers – a review
... AFLP analysis is the short time required to assay large numbers of DNA ...AFLP analysis is the effec- tively unlimited number of loci that can be assayed with different combinations of a relatively small ...
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-RFLP, -RAPD -AFLP,
... Restriction Fragment Length Polymorphism (RFLP) is a difference in homologous DNA sequences that can be detected by the presence of fragments of different lengths after digestion of the DNA ...
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hsp65 Sequencing for Identification of Rapidly Growing Mycobacteria
... The taxonomy of RGM was not the main focus of this study, but phylogenetic trees constructed based on hsp65 and 16S rRNA sequences were compared. Figure 2 shows the hsp65 phylogenetic tree obtained with the 10 type ...
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