Reverse Genetics
A reverse genetics system for avian coronavirus infectious bronchitis virus based on targeted RNA recombination
... In order to study its characteristics, several research groups have independently developed a reverse genetics system (RGS) for IBV which allow the manipulation of its genome [22 – 26]. All these systems ...
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Reverse Genetics for Crimean-Congo Hemorrhagic Fever Virus
... successful reverse genetics system for ...virus-dependent reverse genetics system ...bunyavirus reverse genetics system ...virus-driven reverse genetics system ...
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Development and use of avian pneumovirus reverse genetics systems
... The ability to create a virus entirely from cDNA is a powerful tool and is the ultimate goal of reverse genetics studies. Using a cloned copy of the viral genome and helper plasmids, it is possible to ...
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A plasmid-based reverse genetics system for influenza A virus.
... of reverse genetics tech- niques (for a review, see reference ...cell. Reverse genetics techniques have allowed the rescue of synthetic vRNA segments into infectious influenza viruses, which ...
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Establishment of reverse genetics system for PPR virus to develop recombinant vaccines
... suitable reverse genetics system for PPRV is available and as such we aimed to establish sequences from different isolates and vaccines strains to ensure that the genome sequence was correct, a ...
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Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas
... and reverse genetics strategies are widely employed in the research of ...Forward genetics study generally composed of four major steps: mutagene- sis, phenotypic screening, mutation loci (gene) ...
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A Reverse Genetics Approach To Study Feline Infectious Peritonitis
... Feline infectious peritonitis (FIP) is a lethal immunopathological disease caused by feline coronaviruses (FCoVs). Here, we de- scribe a reverse genetics approach to study FIP by assessing the pathogenicity ...
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Efficient Reverse Genetics Reveals Genetic Determinants of Budding and Fusogenic Differences between Nipah and Hendra Viruses and Enables Real-Time Monitoring of Viral Spread in Small Animal Models of Henipavirus Infection
... efficient reverse genetics system that increased rescue titers by >3 log units, which offset the difficulty of generating multiple recombinants under con- straining biosafety level 4 (BSL-4) ...
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Reverse Genetics System for Severe Fever with Thrombocytopenia Syndrome Virus
... We first generated an M-segment-based minigenome and as- certained that our nucleocapsid and polymerase protein-express- ing clones were functional. We established the optimum ratio of transfected N- and L-expressing ...
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Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus
... Such reverse genetics sys- tems have been achieved for a number of positive-stranded RNA viruses, with genome sizes ranging from 7 kb (picorna- viruses) to 15 kb (arteriviruses) to 20 kb (Citrus tristeza ...
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A reverse genetics approach to study the pathogenesis of pneumonia virus of mice
... In an attempt to investigate the possible presence of subpopulation of viruses in the working stock of PVM strain J3666, 14 independent clones containing parts of the SH and G genes derived from two separate RT-PCR ...
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Development and Application of a Reverse Genetics System for Japanese Encephalitis Virus
... Prior to establishing the JEV reverse genetics system, we first had to determine the complete nucleotide sequence of CNU/LP2. Long RT-PCR was used to amplify the entire viral RNA genome apart from the 5⬘ ...
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Reverse genetics in Chlamydomonas: a platform for isolating insertional mutants
... high-throughput reverse genetics strategy that has been used in plants to generate allelic series of chemically-induced point muta- tions in genes of ...
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Natural Genetic Variation of Xanthomonas campestris pv campestris Pathogenicity on Arabidopsis Revealed by Association and Reverse Genetics
... Still, conserved invariable genes are not amendable to GWA studies, and conserved T3SPs were studied by reverse genetics in X. campestris pv. campestris 8004. Besides the known pathogenic- ity function of ...
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Reverse Genetics System for Porcine Enteric Calicivirus, a Prototype Sapovirus in the Caliciviridae
... This reverse genetics system for PEC provides a new tool to study the molecular basis of replication and pathogenesis for caliciviruses associated with diarrheal ...
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REVERSE-GENETICS BY TILLING EXPANDS THROUGH THEPLANT KINGDOM
... The reverse-genetics strategy known as TILLING (Targeting Induced Local Lesions IN Genomes) was created to harness the power of induced mutations to target and recover lesions in specific ...for ...
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Reverse Genetics Generation of Chimeric Infectious Junin/Lassa Virus Is Dependent on Interaction of Homologous Glycoprotein Stable Signal Peptide and G2 Cytoplasmic Domains
... The Arenaviridae are a diverse and globally distributed collection of viruses that are maintained primarily by rodent reservoirs. Junin virus (JUNV) and Lassa virus (LASV) can both cause significant outbreaks of severe ...
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Requirements and comparative analysis of reverse genetics for bluetongue virus (BTV) and African horse sickness virus (AHSV)
... Reverse genetics (RG) using expression plasmids and in vitro synthesized RNAs is very similar to the method using exclusively in vitro synthesized RNAs [12, ...
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PCR based reverse genetics strategy for bluetongue virus recovery
... Current reverse genetics (RG) strategy to rescue BTV mainly rely on in vitro synthesis of RNA transcripts from cloned complimentary DNA (cDNA) corresponding to viral genome segments with the aid of helper ...
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A Reverse Genetics Platform That Spans the Zika Virus Family Tree
... South Pacific and epidemic Latin American strains (5). The first ZIKV cases in Brazil were reported in early 2015, with the outbreak initially concentrated in the northeastern region of the country (6, 7). Although early ...
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