Reverse phase protein arrays
Increasing the sensitivity of reverse phase protein arrays by antibody-mediated signal amplification
... Background: Reverse phase protein arrays (RPPA) emerged as a useful experimental platform to analyze biological samples in a high-throughput ...
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A Technical Assessment of the Utility of Reverse Phase Protein Arrays for the Study of the Functional Proteome in Non-microdissected Human Breast Cancers
... vidual protein variability resulting from intratumoral ...applying reverse phase protein arrays (RPPA) to protein lysates derived from two independent sections obtained from each ...
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RNAi-based validation of antibodies for reverse phase protein arrays
... total protein concentration was ...and protein concentration was observed for the nine anti-STAT3 antibodies over a >20-fold concentration ...target protein-specific, a STAT3- knockdown was ...
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Reverse phase protein arrays in signaling pathways: a data integration perspective
... the reverse phase protein array (RPPA) data platform provides relative abundances for a set of key proteins (either total proteins or posttranslationally modified proteins), 4 and this platform is ...
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An efficient procedure for protein extraction from formalin-fixed, paraffin-embedded tissues for reverse phase protein arrays
... scaled protein concentrations were normalized by global sample median normalization (GSMN) (the median of all protein marker intensities for a single sample is subtracted from each of the data points of ...
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Spatiotemporal expression profiling of proteins in rat sciatic nerve regeneration using reverse phase protein arrays
... in protein levels over baseline at 21 and 28 days in the proximal segment (q-values ...versican protein levels were elevated overall although the change was only sig- nificant in the distal segment ...
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Reverse phase protein array based tumor profiling identifies a biomarker signature for risk classification of hormone receptor-positive breast cancer
... 2.2. Reverse phase protein arrays Frozen tumor samples were homogenized using a bead mill (TissueLyser, Qiagen) and tissue protein extraction reagent (T-PER, Thermo Scientific) ...
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Development of multiplexed protein profiling and detection using near infrared detection of reverse-phase protein microarrays
... Abstract Protein microarrays have been recently employed for signal pathway profiling and high- throughput protein expression ...analysis. Reverse- phase arrays, where the array ...
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Development of reverse phase protein microarrays for the validation of clusterin, a mid-abundant blood biomarker
... replicate arrays were analysed resulting in 596 sets of quadruplicates or 2384 data points in total (Figure ...four arrays was median normalized as previously described (methods), and for each sample, the ...
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Quantitative analysis of proteins of metabolism by reverse phase protein microarrays identifies potential biomarkers of rare neuromuscular diseases
... recombinant protein in most cases due to the tag used for ...of protein in HCT116 ...the arrays (see Figure ...the protein interrogated in the arrays. Protein concentrations in ...
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Protein Functionalized Nanodiamond Arrays
... (SAMs) arrays adsorbed on silicon and ITO substrates surface using electron beam lithography ...nanodiamond arrays were functionalized with lysozyme to target a cer- tain biomolecule or protein ...
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NORMAL AND REVERSE PHASE CHROMATOGRAPHY.ppt
... stationary phase is a solid and the solutes are it is effective if the stationary phase is a solid and the solutes are adsorbed on its adsorbed on its ...
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Theory of HPLC Reverse Phase Chromatography
... Table 2: Mobile phase viscosity at 25 °C for reversed phase systems. 2 The solvent must be stable for long periods of time. This disfavors tetrahydrofuran which, after exposure to air, degrades rapidly, ...
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Semi-quantitative measurement of specific proteins in human cumulus cells using reverse phase protein array
... and the optical density of the signals was measured with ImageJ software (National Institutes of Health, USA). Reverse phase protein array (RPPA) For each transfection, a five-fold dilution of cell ...
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Protein patterns template arrays of magnetic nanoparticles
... amphiphilic protein found in magnetosome membranes in Magnetospirillum magneticum strain AMB- 1, can control the morphology of magnetite nanoparticles, both in vivo and in ...and protein resistant ...
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Multiplexed Nucleic Acid Programmable Protein Arrays
... as protein-protein interactions, protein-DNA interactions, discovery of drug binding target as well as (auto)antibody biomarkers for a variety of human ...investigating protein functions or ...
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PrepTip. Reverse Phase PrepTip User Guide
... Using the PrepTip RP, aspirate and dispense 10µl* of sample one time** to allow the biomolecules to bind.. While samples can be applied directly to the PrepTip RP, addition of 1% triflu[r] ...
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Hybrid beamforming for large antenna arrays with phase shifter selection
... employing phase shifters with resolution more than 2-bits, can approach the performance of a similar system with analog phase ...novel phase shifter selection scheme was ...the phase shifters ...
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Explicit Calibration of mmwave Phased Arrays with Phase Dependent Errors
... the phase and gain errors in the phased array; by knowing these errors the codebook for the phases applied on the antennas can be designed so that the beams point in the intended directions and a good coverage ...
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Assessment of a panel of tumor markers for the differential diagnosis of benign and malignant effusions by well-based reverse phase protein array
... the protein content in the fluid compartment, we measured MUC1, EMA, Pan- CK, HSP90, TGF-β and CA125 by a well-based RPPA methodology in pleural effusions and ...
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