Reverse Transcriptase PCR
Detection and Genotyping of Human Rotavirus VP4 and VP7 Genes by Reverse Transcriptase PCR and Reverse Hybridization
... novel reverse transcriptase PCR (RT-PCR) method for the amplification of rotavirus RNA and a reverse hybridization assay on a strip to detect amplimers and identify the specific G and P ...
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Hepatitis C Virus Subtyping by a Core Envelope 1 Based Reverse Transcriptase PCR Assay with Sequencing and Its Use in Determining Subtype Distribution among Danish Patients
... A reverse transcriptase PCR (RT-PCR) assay using conserved primers deduced from the core-envelope 1 (C-E1) region of the hepatitis C virus (HCV) genome was developed for subtyping ...
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Multiplex reverse transcriptase pcr assay for the detection of infective stage wuchereria bancrofti and its vector culex quinquefasciatus
... specific PCR assay was developed at VCRC for detecting the presence or absence of infective stage in the vector, Culex quinquefasciatus (Vasuki et ...simple reverse transcriptase-PCR ...
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Simultaneous Detection of Rift Valley Fever, Bluetongue, Rinderpest, and Peste des Petits Ruminants Viruses by a Single Tube Multiplex Reverse Transcriptase PCR Assay Using a Dual Priming Oligonucleotide System
... virus-specific PCR-based assays have been devel- oped as an alternative to virus isolation for the diagnosis of individual ...(e.g., PCR methods) allow rapid and sensitive detection of some of the most ...
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Fast Duplex One Step Reverse Transcriptase PCR for Rapid Differential Detection of West Nile and Japanese Encephalitis Viruses
... duplex reverse transcriptase PCR (RT-PCR) assay for the simultaneous and differential detection of West Nile (WNV) and Japanese encephalitis (JEV) ...duplex PCR using these primers is a ...
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Single tube, nested, reverse transcriptase PCR for detection of viable Mycobacterium tuberculosis
... as PCR, are widely accepted for the detection of infectious ...a reverse transcriptase PCR (RT-PCR), a positive signal would indicate the presence of a recently viable ...DNA PCR ...
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Comparison of Enzyme Immunoassay and Reverse Transcriptase PCR for Identification of Serotype G9 Rotaviruses
... by reverse transcriptase PCR (RT-PCR) under code for the determination of the G types of rotaviruses obtained from neonates in New Delhi, ...
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Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
... We have developed and evaluated a real-time reverse transcriptase PCR (RT-PCR) assay for the detection of human enterovirus D68 (EV-D68) in clinical specimens. This assay was developed in ...
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Detection of Treponema pallidum by a sensitive reverse transcriptase PCR
... several PCR methods have been developed for the detection of ...sensitive reverse transcriptase PCR (RT-PCR) that targets a 366 bp region of the 16S rRNA of ...
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Rapid diagnosis of Argentine hemorrhagic fever by reverse transcriptase PCR based assay
... of the possible false positives (94%, 15 of 16) came from patients who had FSUE (69%, 11 of 16) or presumptive clinical AHF (25%, 4 of 16). To test these hypotheses, we carried out two specificity tests. First, we tested ...
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Detection of Human Immunodeficiency Virus Type 1 Antiretroviral Resistance Mutations by High Density DNA Probe Arrays
... nested reverse transcriptase PCR, and detection with high-density probe arrays designed to detect 204 antiretroviral resistance mutations simultaneously in Gag cleavage sites, protease, ...
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In vivo cytokine profiles in patients with kala azar Marked elevation of both interleukin 10 and interferon gamma
... In the present study, we assessed in vivo cytokine patterns in patients with visceral leishmaniasis using a quantitative reverse transcriptase PCR method for the amplification of cytokin[r] ...
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Truncated Particles Produced in Fish Surviving Infectious Hematopoietic Necrosis Virus Infection: Mediators of Persistence?
... Infectious hematopoietic necrosis virus (IHNV) is a rhabdovirus that produces an acute, lethal infection in rainbow trout (Oncorhynchus mykiss). Fish that survive infection cease to produce detectable infectious virus at ...
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Human skin identification using specific gene marker at different storage temperatures
... conserved genes that encode stratum corneum proteins (Jackson et al., 2005). LCE genes, including late cornified envelope 1D (LCE1D), are abundant and highly sensitive skin biomarkers. Molecular methodologies have been ...
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The Gene Search System: A Method for Efficient Detection and Rapid Molecular Identification of Genes in Drosophila melanogaster
... We have constructed a P-element-based gene search vector for efficient detection of genes in Drosophila melanogaster. The vector contains two copies of the upstream activating sequence (UAS) enhancer adjacent to a core ...
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Characterization of the murine n-formyl peptide chemotactic receptor
... expressed in murine FDCP cells differentiated to neutrophils, was identified from six.. putative genes by reverse transcriptase PCR.[r] ...
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Molecular biology techniques and applications for ocean sensing
... and reverse- transcriptase PCR, quantitative PCR, whole assemblage “fin- gerprinting” approaches (based on nucleic acid sequence or length heterogeneity), oligonucleotide microarrays, and ...
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Identification and Genomic Characterization of a New Virus (Tymoviridae Family) Associated with Citrus Sudden Death Disease
... by PCR or reverse transcriptase PCR (RT-PCR) using the cDNA libraries or total RNA from CSD-affected plants as templates, a combination of internal forward and reverse primers ...
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A TaqMan Probe Based Real Time PCR Assay for the Rapid Identification of the Emerging Multidrug Resistant Pathogen Candida auris on the BD Max System
... C. auris is often misidentified as the closely related species Candida haemulonii by traditional microbiological methods, such as chromogenic agar, analytical profile index (API) strips, or automated systems such as the ...
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Detection and identification of aquatic birnaviruses by PCR assay
... the PCR assay with PrB detected virus in infected cell cultures, amplification products from the PCR assay with PrB either were not detected in the clinical speci- mens or appeared as large, diffuse bands ...
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