RFLP analysis
Genotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis
... In RFLP analysis of PCR products with AluI, they reported six different restriction patterns (A-F) with pattern B (80-180-210) as the most common ...in RFLP analysis of the coa gene variants ...
6
Comparative Evaluation of Ligation Mediated PCR and Spoligotyping as Screening Methods for Genotyping ofMycobacterium tuberculosis Strains
... (RFLP) analysis with IS6110 (IS6110 RFLP analysis) as a “gold standard,” we performed a comparative evaluation of spoligotyping and ligation-mediated PCR (LMPCR), a recently described ...
6
Stock Structure and Homing Fidelity in Gulf of Mexico Sturgeon (Acipenser oxyrinchus desotoi) Based on Restriction Fragment Length Polymorphism and Sequence Analyses of Mitochondrial DNA
... Population structure: RFLP analysis of the entire mtDNA genome and direct sequencing analysis of the mtDNA control region provided congruent results and indicated extensiv[r] ...
9
Leptin receptor gene as a genetic marker for leanness in pigs
... When this analysis is conducted and the polymorphism is determined by PCR RFLP analysis using the restriction endonuclease HinfI, or MboI and ampli?cation primers may be designed [r] ...
15
Mitochondrial DNA Heteroplasmy in Wheat, Aegilops and Their Nucleus-Cytoplasm Hybrids
... PCR-RFLP analysis and Southern blot analysis using the b1 copy as a probe showed the presence of stoichiometrically different sequence copies in the hy- brids; more maternal copies were detected in ...
12
Low Genetic Variation Suggest Single Stock of Kawakawa Euthynnus affinis (Cantor, 1849) along the Indian Coast
... (RFLP) analysis of the mitochondrial D-loop region was employed to examine the levels of genetic diversity among kawakawa samples collected from eight main fishing zones (Veraval (VE), Ratnagiri (RA), Kochi ...
10
Identification and Genotyping of Mycobacterium tuberculosis Complex Species by Use of a SNaPshot Minisequencing Based Assay
... by analysis of the phenotypic and bio- chemical characteristics of the bacteria after ...the analysis of genomic deletions by PCR, followed by agarose gel electro- phoresis (30, 40, 56), or are based on the ...
9
Selection of a Teicoplanin Resistant Enterococcus faecium Mutant during an Outbreak Caused by Vancomycin Resistant Enterococci with the VanB Phenotype
... The RFLP analysis of vanB gene clusters present in out- break isolates demonstrated that all the VREM isolates con- tained the same cluster RFLP type, which had been identified before as ...
9
Characterization of a novel Rochalimaea species, R henselae sp nov , isolated from blood of a febrile, human immunodeficiency virus positive patient
... Both 16S rRNA gene sequence analysis and PCR-RFLP analysis of the citrate synthase gene led to the conclusion that the Houston-1 isolate, while having similarities to species recognized [r] ...
10
Fast Ligation Mediated PCR, a Fast and Reliable Method for IS6110 Based Typing of Mycobacterium tuberculosis Complex
... (RFLP) analysis is the most widely applied method for strain differentiation of Mycobacterium tuberculosis ...allows analysis of strains within one working day and starting from less than 1 ng of ...
6
Stability of Variable Number Tandem Repeats of Mycobacterial Interspersed Repetitive Units from 12 Loci in Serial Isolates of Mycobacterium tuberculosis
... combines analysis of four multiplex PCRs for the 12 loci on a fluorescence-based DNA analyzer with computerized automation of the ...genotyping. Analysis of a blinded reference set of strains by our ...
6
Evaluation of Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Analysis and Spoligotyping for Genotyping of Mycobacterium bovis Isolates and a Comparison with Restriction Fragment Length Polymorphism Typing
... endonuclease analysis (REA), restriction fragment length polymorphism (RFLP) analysis, spoligotyping, and, more recently, mycobacterial interspersed repetitive-unit–variable-number tandem-repeat ...
5
Molecular Fingerprinting of Mycobacterium bovis subsp caprae Isolates from Central Europe
... IS6100 RFLP analysis, which identified 43 different RFLP types (designated rc1 to rc43) ...prominent RFLP types among all isolates were rc1, whose four-band pattern was en- countered 19 times, ...
5
Genetic Diversity of Mycobacterium africanum Clinical Isolates Based on IS6110 Restriction Fragment Length Polymorphism Analysis, Spoligotyping, and Variable Number of Tandem DNA Repeats
... (IS6110- RFLP) analysis, IS1081-RFLP analysis, direct repeat-based spacer oligonucleotide typing (spoligotyping), variable number of tandem DNA repeats (VNTR), and the polymorphism of the ...
9
Identification and Differentiation of Leishmania Species in Clinical Samples by PCR Amplification of the Miniexon Sequence and Subsequent Restriction Fragment Length Polymorphism Analysis
... Our assay was developed for use in a diagnostic setting where patients with a history of global travel are seen. It was the aim of this study to optimize and evaluate a recently de- veloped PCR-RFLP-based ...
7
Genetic Divergence between Auxis thazard and A. rochei based on PCRRFLP Analysis of mtDNA D-loop Region
... Tunas of genus Auxis are commercially and ecologically important fish of family Scombridae. There is currently no information concerning the genetic divergence between A. thazard and A. rochei. Therefore, a study was ...
8
Detection of Streptococcus pneumoniae Strain Cocolonization in the Nasopharynx
... Colonization with more than one distinct strain of the same species, also termed cocolonization, is a prerequisite for horizontal gene transfer between pneumococcal strains that may lead to change of the capsular ...
7
Efficient Tracing of Global Isolates of Yersinia pestis by Restriction Fragment Length Polymorphism Analysis Using Three Insertion Sequences as Probes
... 3IS-RFLP analysis per- formed here demonstrate that the two strains recently isolated in Surat have identical 3IS types, confirming that they both derive from the epidemic clone which caused the pneumonic ...
9
Malassezia arunalokei sp nov , a Novel Yeast Species Isolated from Seborrheic Dermatitis Patients and Healthy Individuals from India
... Taxonomy. Malassezia arunalokei Honnavar, Rudramurthy, and Prasad, sp. nov. MycoBank accession no. MB 808048. Ety- mology: Malassezia arunalokei, the species epithet arunalokei (a.ru.na.lo’ke.i. N.L. masc. gen. n. ...
9
Evaluation of Methods for Subtyping Campylobacter jejuni during an Outbreak Involving a Food Handler
... (RFLP) analysis of flaA, DNA sequence analysis of 585 bp of flaA that included the short variable region (SVR), sequencing of the entire flaA gene, and PFGE using SmaI and ...
5