RING E3 ubiquitin ligase
Role of TRIM5α RING Domain E3 Ubiquitin Ligase Activity in Capsid Disassembly, Reverse Transcription Blockade, and Restriction of Simian Immunodeficiency Virus
... TRIM5 RING E3 ubiquitin ligase activity (data not ...the ubiquitin-charged E1 enzyme and other potential cellular contaminants, the anti-HA Western blot was used for quantification of ...
17
The structure and regulation of Cullin 2 based E3 ubiquitin ligases and their biological functions
... Cullin-RING E3 ubiquitin ligase complexes; Cul2: Cullin-2; Rbx1: RING protein; ECS: Elongin B and C-Cul2 or Cul5-SOCS box protein; VHL: von Hip- pel–Lindau; HIFα: α subunits of hypoxia ...
11
Rotavirus NSP1 Associates with Components of the Cullin RING Ligase Family of E3 Ubiquitin Ligases
... To further support the siRNA knockdown data, we turned again to the dominant-negative Cul3 mutant, which does not con- tain the Rbx1 binding site and, by extension, also does not bind to the E2 ...
13
The ubiquitin E3 ligase activity of BRCA1 and its biological functions
... a RING heterodimer with BARD1 to provide ubiquitin E3 ligase activity that is required for its tumor suppressor ...BRCA1 RING finger is capable of catalyzing multiple types of ...
10
Autographa californica Nucleopolyhedrovirus AC141 (Exon0), a Potential E3 Ubiquitin Ligase, Interacts with Viral Ubiquitin and AC66 To Facilitate Nucleocapsid Egress
... by ubiquitin (Ubi) ...between ubiquitin-activating E1 enzymes and the C-terminal glycine of ...activated ubiquitin is transferred to a ubiquitin-conjugating E2 ...of ubiquitin from E2 ...
21
Epigenetic reprogramming in mouse pre implantation development and primordial germ cells
... RNF4 RING finger protein 4, a SUMO-dependent ubiquitin E3-ligase implicated in the maintenance of genome stability, has also been shown to have a role in active DNA demethylation both in[r] ...
17
Characterization of a Novel RING Type Ubiquitin E3 Ligase GhRING2 Differentially Expressed in Cotton Fiber
... one E3 gene (GhRING1 encoding a RING-type ubiquitin ligase), and studied their expression in cotton fiber [20] ...the ubiquitin E3 ligase activity by an in vitro ...
17
The ORF3 Protein of Porcine Circovirus Type 2 Interacts with Porcine Ubiquitin E3 Ligase Pirh2 and Facilitates p53 Expression in Viral Infection
... Porcine circovirus type 2 (PCV2) is the primary causative agent of an emerging swine disease, postweaning multisystemic wasting syndrome. We previously showed that a newly identified protein, ORF3, plays a major role in ...
8
Contribution of E3-Ubiquitin Ligase Activity to HIV-1 Restriction by TRIM5αrh: Structure of the RING Domain of TRIM5α
... of RING mu- tant proteins inversely correlated with the degree of ...the RING-RING interaction region that re- moved the ability to undergo self-ubiquitylation might cause a defect in RING ...
13
Varicella-Zoster Virus Inhibition of the NF-κB Pathway during Infection of Human Dendritic Cells: Role for Open Reading Frame 61 as a Modulator of NF-κB Activity
... its RING finger domain. ORF61 Q139_STOP is truncated following the E3 ubiquitin ligase domain, ORF61 Q62_START is transcribed following the RING finger domain, and ORF61 C19G contains ...
10
Ubiquitin E3 ligase MARCH7 promotes ovarian tumor growth
... membrane-associated RING-CH (MARCH) proteins belong to the RING finger protein family of E3 ubiquitin ligases, consisting of 11 members in ...single RING finger domain ...
14
The RING Finger Domain of Varicella-Zoster Virus ORF61p Has E3 Ubiquitin Ligase Activity That Is Essential for Efficient Autoubiquitination and Dispersion of Sp100-Containing Nuclear Bodies
... ICP0’s E3 ubiquitin ligase activity, which is required for autoubiquitination and targeting host proteins for proteasomal degradation, maps to its RING finger domain (1, 7) and, re- cently, a ...
5
Virus-Specific Effects of TRIM5αrh RING Domain Functions on Restriction of Retroviruses
... vitro E3 ubiquitin ligase activity assay in the absence and presence of ...the E3 ubiquitin ligase activity of TRIM5 ␣ rh RING domain variants and ...
12
Putative E3 Ubiquitin Ligase of Human Rotavirus Inhibits NF κB Activation by Using Molecular Mimicry To Target β TrCP
... intact RING domain, is sufficient to target SCF -TrCP and that SA11-4F and OSU NSP1 proteins conserve an architecture and mechanism similar enough to permit this transfer of ...
13
E3 ubiquitin ligases and their control of T cell autoreactivity
... conserved RING) functions as a dominant negative mutant capable of inhibiting endogenous GRAIL function and blocking the development of anergy ...H2N2 RING mutants also fail to suppress IL-2 secretion in ...
10
Comparison of the Biological and Biochemical Activities of Several Members of the Alphaherpesvirus ICP0 Family of Proteins
... an E3 ubiquitin ligase of the RING finger class that is required for efficient lytic infection and reactivation from ...ICP0-related RING finger proteins, but these have limited ...
12
Modulating Parkin E3 Ubiquitin Ligase Activity Using Phospho-Ubiquitin Variants
... Human parkin is a 52 kDa protein with 465 amino acid residues 9 . It consists of five domains: Ub-like (Ubl) (1-76), really interesting new gene (RING) 0 (144-216), RING1 (228-327), in-between-ring (IBR) ...
95
Degradation of Centromeric Histone H3 Variant Cse4 Requires the Fpr3 Peptidyl-prolyl Cis–Trans Isomerase
... Psh1 ubiquitinates Cse4 at the following lysine residues in its C terminus: K131, K155, K163, and K172 (Hewawasam et al. 2010). As well, Cse4 directly interact with Psh1 via the RING finger domain, which is a ...
15
DNA Replication Origin Function Is Promoted by H3K4 Di-methylation in Saccharomyces cerevisiae
... The Set1 histone methyltransferase and the Bre1 E3 ubiquitin ligase have well-established roles in regulating gene expression (Shukla et al. 2006; Mutiu et al. 2007). Nonetheless, several lines of ...
14
The KCTD family of proteins: structure, function, disease relevance
... There are some features of KCTDs that have not been reviewed in this article. For example, KCTD8, -12, -12b, and-16 form functional oligomers with the GABAB recep- tor, resulting in the modulation of important signaling ...
5