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RNA Amount from Human Brain Tissue

Isolation of Borna Disease Virus from Human Brain Tissue

Isolation of Borna Disease Virus from Human Brain Tissue

... The source of BDV and routes for human infection are unknown. Infected blood cells, both lymphocytes and macro- phages, are thought to facilitate access to the CNS by several neurotropic viruses. BDV RNA ...

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On the Fractal Design in Human Brain and Nervous Tissue

On the Fractal Design in Human Brain and Nervous Tissue

... This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/ Abstract Digital imaging techniques have enabled to gain insight into complex ...

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In Situ Hybridization Detection of Human Herpesvirus 6 in Brain Tissue From Fatal Encephalitis

In Situ Hybridization Detection of Human Herpesvirus 6 in Brain Tissue From Fatal Encephalitis

... CASE REPORT A 23-month-old girl was admitted to the regional hospital because of rash, fever, and irritability. She was born after a full- term pregnancy. Since 12 months of age, the child had been followed up because of ...

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Genome-wide DNA methylation analysis of human brain tissue from schizophrenia patients

Genome-wide DNA methylation analysis of human brain tissue from schizophrenia patients

... Our data indicate that studies of epigenetic changes in schizophrenia hold promise for the future development of diagnostic and prognostic biomarkers for schizophrenia as well as therapeutic options that target causative ...

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Leukemia- Derived Exosomes Induce Migration and Tumor Initiating in Astrocytes from Human Brain Tissue

Leukemia- Derived Exosomes Induce Migration and Tumor Initiating in Astrocytes from Human Brain Tissue

... 5- CONCLUSION In short, we studied the effect of exosomes derived from leukemia on the CNS through human astrocyte cells to investigate the expression of some cancer- related genes. The results can have ...

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TISSUE-ENGINEERED MICROVESSEL MODELS OF THE HUMAN BLOOD-BRAIN BARRIER

TISSUE-ENGINEERED MICROVESSEL MODELS OF THE HUMAN BLOOD-BRAIN BARRIER

... of human iPSC-derived blood-brain barrier microvessels resembling in vivo post-capillary ...generated from multiple iPS cell lines and display localization of tight junction proteins, and expression ...

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Ganglioside Detection from Formalin Fixed Human Brain Tissue Utilizing MALDI Imaging Mass Spectrometry

Ganglioside Detection from Formalin Fixed Human Brain Tissue Utilizing MALDI Imaging Mass Spectrometry

... clinical tissue through the use of an aqueous wash, further expanding changes observed within these ...fixed brain tissue in particular will allow for further studies of clinical brain samples ...

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Next-generation sequencing analysis and RNA editing in human brain and glioma

Next-generation sequencing analysis and RNA editing in human brain and glioma

... WORK RNA sequencing has changed our view of transcriptomics in several ...of RNA species that are present in the cell, rather than the more restrictive probe-­‐based queries of ...with RNA-­‐seq, ...

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Pre   proparathyroid hormone identified by cell   free translation of messenger RNA from hyperplastic human parathyroid tissue

Pre proparathyroid hormone identified by cell free translation of messenger RNA from hyperplastic human parathyroid tissue

... differs from that of both proparathyroid hormone and parathyroid ...of human parathyroid hormone, pre-proparathyroid hormone, analogous to that identified recently by in vitro translation of bovine ...

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Methylomic profiling of human brain tissue supports a neurodevelopmental origin for schizophrenia

Methylomic profiling of human brain tissue supports a neurodevelopmental origin for schizophrenia

... cerebellum brain tissue from schizophrenia patients and controls, identifying disease-associated differential DNA methylation at multiple loci, particularly in the prefrontal cortex, and confirming ...

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Measuring cell type specific differential methylation in human brain tissue

Measuring cell type specific differential methylation in human brain tissue

... scarce tissue bank resources and limited funds for methylation ...obtained from the brain regions of interest using a small number of samples, the gold-standard methylation data can be used for any ...

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Aging effects on DNA methylation modules in human brain and blood tissue

Aging effects on DNA methylation modules in human brain and blood tissue

... statistics are inferior for the purposes of our study. While cross-tabulation approaches are intuitive, they have sev- eral disadvantages. To begin with, they are only applic- able if the module assignment in the test ...

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Functional annotation of the human brain methylome identifies tissue specific epigenetic variation across brain and blood

Functional annotation of the human brain methylome identifies tissue specific epigenetic variation across brain and blood

... multiple brain regions and blood, we have identified key regions of the genome that are character- ized by tissue-specific patterns of DNA ...data from other studies, we find striking evidence that ...

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Simultaneous DNA and RNA isolation from brain punches for epigenetics

Simultaneous DNA and RNA isolation from brain punches for epigenetics

... and RNA expression both should be determined within the same ...methylation from two separate cohorts of animals may introduce a bias, unless at least double the numbers of animals are included in each ...

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Sodium channels from human brain RNA expressed in Xenopus oocytes  Basic electrophysiologic characteristics and their modification by diphenylhydantoin

Sodium channels from human brain RNA expressed in Xenopus oocytes Basic electrophysiologic characteristics and their modification by diphenylhydantoin

... channels from human brain RNA in the Xenopus ...expressed human sodium channel by DPH can be described by models previously developed to explain block of Na channels by local ...

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Human herpesvirus multiplex ddPCR detection in brain tissue from low- and high-grade astrocytoma cases and controls

Human herpesvirus multiplex ddPCR detection in brain tissue from low- and high-grade astrocytoma cases and controls

... clinical tissue specimens were collected either as FFPE, OCT, or fresh frozen biopreservation ...any tissue specimens when assayed by either a singleplex or multiplex ...disease tissue spe- cimen ...

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Highly efficient method for isolation of total RNA from adipose tissue

Highly efficient method for isolation of total RNA from adipose tissue

... total RNA isolation from adipose tissue, the miR- Neasy method results in cleaner samples but more prone to degradation and the TRI Reagent W method results in samples contaminated with salts and ...

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Comparison of RNA isolation from FFPE tissue on two different platforms

Comparison of RNA isolation from FFPE tissue on two different platforms

... FFPE tissue has been used routinely in clinical diagnosis for ...FFPE tissue for identifying new molecular will not only aid diagnosis, but also guide treatment of ...mRNA from FFPE was shown as a ...

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A single-nuclei RNA sequencing study of Mendelian and sporadic AD in the human brain

A single-nuclei RNA sequencing study of Mendelian and sporadic AD in the human brain

... the human brain to identify the colocalization of Aβ particles with SPP1, a gene identified as a DAM marker ...the human brains, including the analyses of bulk RNA-seq from homogenized ...

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The effect of prolonged formalin fixation on the staining characteristics of archival human brain tissue

The effect of prolonged formalin fixation on the staining characteristics of archival human brain tissue

... large amount of brain tissues are fixed but little is known about whether they are suitable for retrospective ...archival brain specimens. Materials and methods: Twenty brain specimens were ...

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