RNA-binding
The RNA binding landscapes of two SR proteins reveal unique functions and binding to diverse RNA classes
... the binding leads to alternative splicing changes that increase the occurrence of PTCs, which in turn target the expressed transcripts for degradation through the NMD ...other RNA binding proteins ...
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High affinity RNA binding by a hyperthermophilic single stranded DNA binding protein
... an RNA-binding module, as RNA is thought to have predated DNA early in evolution (Orgel 1998), and several examples of OB fold domains spe- cialised for RNA binding have been ...
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Ebola Virus VP30 Is an RNA Binding Protein
... The RNA interacting domain of VP30 maps to N ...2⫹ binding motif as mentioned previously (25, ...for RNA at low pH values, suggested a role for the N terminus in this ...with RNA at a ...
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Structural Phosphoprotein M2-1 of the Human Respiratory Syncytial Virus Is an RNA Binding Protein
... FIG. 4. M2-1 protein deletion variants and their RNA binding capacities determined by Northern-Western assays. (A) Diagram representation of M2-1 protein indicating the amino acid residues deleted in the ...
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Multimerization of Hepatitis Delta Antigen Is a Critical Determinant of RNA Binding Specificity
... the RNA as a preformed multimer or whether binding is pre- ceded by multimer dissociation, we compared RNP complexes formed by HDAg-160, HDAg-145, an additional truncated form of the protein, and a mixture ...
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RNA binding properties of poliovirus subviral particles.
... immobilized RNA molecules select RNA-binding proteins from protein preparations (5, 14) was used to detect interactions between putative viral assembly intermediates and the poliovirus RNA ...
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The matrix domain contributes to the nucleic acid chaperone activity of HIV 2 Gag
... PM binding [5, ...with RNA in HIV-1 [21, 48–50]. RNA binding to the MA domain ensures the specificity of HIV-1 Gag interactions with PM phospholipids [6, 21, 48, ...in RNA ...
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The NS4A Protein of Hepatitis C Virus Promotes RNA-Coupled ATP Hydrolysis by the NS3 Helicase
... exhibits RNA- stimulated NTPase and helicase activity, while the N-terminal serine protease domain of NS3 enhances RNA binding and unwinding by ...bind RNA in the presence of ATP, thereby ...
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Crystal structure of Schmallenberg orthobunyavirus nucleoprotein RNA complex reveals a novel RNA sequestration mechanism
... in RNA binding Beside the RNA-binding cleft, the N-terminal arm of N also contributes to RNA ...the RNA from exposure to the outside of the ...abrogated RNA-binding ...
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In Vitro Selection of RNA Binding Peptides
... Peptide affinities were determined by fluorescence titration against 2-aminopurine (2AP) labeled boxBR. 2-aminopurine is a fluorescent nucleoside analog of adenine that has been previously substituted in RNA (23). ...
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Hepatitis C Virus Nonstructural Protein 5A: Biochemical Characterization of a Novel Structural Class of RNA-Binding Proteins
... with RNA and to elucidate the minimal requirements ...8). Binding of “specific” (G/U) RNA shifts the equilibrium in favor of dimer; binding of other RNAs does not ...specific RNA ...
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Double-Stranded RNA Binding May Be a General Plant RNA Viral Strategy To Suppress RNA Silencing
... acid binding protein whose binding affinity increases with the length of the RNA ...our binding experiments strongly suggest that in planta-expressed P21 is a size-selective ...
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Lentivirus Tat proteins specifically associate with a cellular protein kinase, TAK, that hyperphosphorylates the carboxyl-terminal domain of the large subunit of RNA polymerase II: candidate for a Tat cofactor.
... TAR RNA element. Once bound to TAR RNA, TAK phosphorylates the CTD of the large subunit of RNAP II in the preinitiation ...HIV RNA, it is proposed that the transcription complexes become modified by ...
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Major contribution of the RNA binding domain of NS1 in the pathogenicity and replication potential of an avian H7N1 influenza virus in chickens
... viral RNA-polymerase in a minireplicon ...the RNA-binding domain in essential steps of the virus replication cycle, notably expression and translation of viral ...
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Widespread RNA binding by chromatin associated proteins
... DNMT1 binding to RNA at the CEBPA loci, we observed lower levels of DNA methylation in promoters of genes bound by DNMT1 genome-wide at all abundance levels ...DNMT1 binding does not appear to affect ...
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Evaluation of RNA binding motif protein 3 expression in urothelial carcinoma of the bladder: an immunohistochemical study
... messenger RNA (mRNA) stabil- ity and translation and to prevent apoptosis during cell mitosis (a mitotic catastrophe) [12], and to also be in- volved in p53-linked DNA damage repair ...
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Trypanosoma brucei RRM1 Is a Nuclear RNA Binding Protein and Modulator of Chromatin Structure
... enriched, many encoded RNA-binding proteins. The most highly enriched transcript was RBP26 (95-fold enrichment); the transcript for RRM1 itself was enriched 10-fold, and the bloodstream-specific RBP10 was ...
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SpoVG Is a Conserved RNA Binding Protein That Regulates Listeria monocytogenes Lysozyme Resistance, Virulence, and Swarming Motility
... of RNA-binding proteins that coordinate vari- ous aspects of bacterial physiology and are primarily described in Gram-negative bacteria (11, ...noncoding RNA CsrB, which antagonizes its function ...
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A Secreted RNA Binding Protein Forms RNA-Stabilizing Granules in the Honeybee Royal Jelly
... 2019). RNA transfer is mediated by secre- tion and consumption of jelly, which is highly enriched for ...of RNA could drive social immunity against the Varroa mite (Garbian et ...viral RNA fragments ...
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Identification of the RNA-Binding, Dimerization, and eIF4GI-Binding Domains of Rotavirus Nonstructural Protein NSP3
... specific RNA binding properties of NSP3. Most often, RNA binding proteins interact with RNA targets which contain various secondary structures as stem- loops, pseudoknots, bulges, and ...
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