RNA dimerization
Mutant human immunodeficiency virus type 1 genomes with defects in RNA dimerization or encapsidation.
... between RNA strands (23, 27, 32, 35) and which were each known to abolish HIV-1 c dimerization in vitro ...however, RNA gently extracted from native ...genomic RNA dimerization ...
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Opening of the TAR hairpin in the HIV 1 genome causes aberrant RNA dimerization and packaging
... vitro RNA dimerization assays, we previously observed that opening of 5′ TAR (A 5 and B 5 mutations) caused increased dimerization of leader RNA transcripts, whereas the AB 5 transcripts ...
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Murine leukemia virus RNA dimerization is coupled to transcription and splicing processes
... the RNA including the dimer linkage structure (DLS) and thereby allows for better recognition of the DLS by the RNA partner and/or by ...for dimerization compared to complete ...enhance ...
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Bipartite Signal for Genomic RNA Dimerization in Moloney Murine Leukemia Virus
... genomic RNA strands that are stably but noncovalently joined in parallel near their 5 ⴕ ...this dimerization has been shown to depend on a unique RNA stem-loop locus, called the dimer initiation site ...
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Is HIV 1 RNA dimerization a prerequisite for packaging? Yes, no, probably?
... between dimerization and packaging, the fact remains that the RNA signals that are important for both of these activities overlap in most retroviral genomes; this makes it difficult to interpret the results ...
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Regulation of primate lentiviral RNA dimerization by structural entrapment
... the dimerization properties of three primate lentiviral RNAs, HIV-2, SIV, and HIV-1, to search for com- mon mechanistic themes in the regulation of SL1-medi- ated ...leader RNA regions of HIV-2 ROD and SIV ...
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Impact of Human Immunodeficiency Virus Type 1 RNA Dimerization on Viral Infectivity and of Stem-Loop B on RNA Dimerization and Reverse Transcription and Dissociation of Dimerization from Packaging
... genome dimerization may cause a 25- fold reduction in viral ...genome dimerization, and little or no impact on genome packaging and other ...genome dimerization displayed by the C258G, UUAA, and ...
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Role of Stem B, Loop B, and Nucleotides next to the Primer Binding Site and the Kissing-Loop Domain in Human Immunodeficiency Virus Type 1 Replication and Genomic-RNA Dimerization
... genomic RNA. We investigated its role in viral replication, genomic RNA dimerization, and dimer- ization of partial HIV-1 RNA ...genome dimerization and all reduced viral replication by ...
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Structural implications of RNA dimerization in a gamma retrovirus
... minimal dimerization active sequence (MiDAS) 7 for a representative gamma retrovirus, the Moloney murine sarcoma virus (MuSV; see ...second RNA molecule via canonical intermolecular C-G base pairing 13 ...
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Mechanism of retroviral genomic RNA dimerization and packaging
... vitro RNA dimerization reveals the structure of the mature ...minimal dimerization domain; however, the dimers formed in vitro in the context of short RNAs, using “near”-physiological ionic and ...
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trans-Acting Inhibition of Genomic RNA Dimerization by Rous Sarcoma Virus Matrix Mutants
... same RNA dimerization defect as Myr1E, virus particles were collected following transfection of QT6 cells with proviral plas- mids and viral RNA was isolated and subjected to North- ern blotting ...
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Monitoring Retroviral RNA Dimerization In Vivo via Hammerhead Ribozyme Cleavage
... including NCp10, are absent in the nonpackaging line, the observed ribozyme activity reflects gag-independent C interac- tions. Therefore, our results are consistent with the existence in vivo of the weak interactions in ...
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Secondary Structure of the Mature Ex Virio Moloney Murine Leukemia Virus Genomic RNA Dimerization Domain
... genomic RNA dimer structures to yield infectious ...genomic RNA dimers even though the relationship between these models and authentic viral RNA is ...minimal dimerization domain in genomes ...
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Studies of the Genomic RNA of Leukosis Viruses: Implications for RNA Dimerization
... of RNA monomers and ...the RNA packaging strategy employed by ...disparate RNA packaging strategies is not clear; however, evolution relies on chance, not ...
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RNA Dimerization Defect in a Rous Sarcoma Virus Matrix Mutant
... Genomic RNA incorporation appears to be mildly reduced compared to the wild-type ...pectedly, RNA isolated from Myr1E particles is monomeric when analyzed on nondenaturing Northern ...the ...
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Role of the DIS hairpin in replication of human immunodeficiency virus type 1.
... HIV-1 RNA dimerization and ...leader RNA from 59 cap to the start of the gag open reading ...of RNA-RNA interaction downstream of the SD. Different kinds of RNA-RNA ...
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The Human Immunodeficiency Virus Type 1 Gag Polyprotein Has Nucleic Acid Chaperone Activity: Possible Role in Dimerization of Genomic RNA and Placement of tRNA on the Primer Binding Site
... genomic RNA (16, 18, 32) and that the primer tRNA is annealed to the PBS on this RNA (11, 17, 23, ...these RNA-RNA interaction events in ...that RNA dimerization occurs without ...
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Sequences Downstream of the 5′ Splice Donor Site Are Required for both Packaging and Dimerization of Human Immunodeficiency Virus Type 1 RNA
... GA-rich RNA sequences at nt 325 to 336 sig- nificantly reduce levels of both viral RNA dimerization and ...GA-rich RNA sequence downstream of the SL3 structure participates in viral RNA ...
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Possible Role of Dimerization in Human Immunodeficiency Virus Type 1 Genome RNA Packaging
... The RNA encapsidation and dimerization abilities of the virions were compared with those of pDDN and the wild-type, pN- ...The RNA-packaging efficiency of DDNBA was compa- rable to those of DDN and ...
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Palindromic Sequence Plays a Critical Role in Human Foamy Virus Dimerization
... retroviral RNA genome is dimeric, consisting of two identical strands of RNA linked near their 5 ⴕ ends by a dimer linkage ...(HFV) RNA transcribed in vitro contained three sites, designated SI, SII, ...
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