RNA Extraction
Rapid plant DNA and RNA extraction protocol using a bench drill
... RNA extraction for genetic diversity and gene expression analyses, where other conventional methods have not been successful due to explant, condition of samples and quantity and quality of nucleic ...
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TRIzol-based RNA Extraction: A Reliable Method for Gene Expression Studies
... RNA extraction is a prerequisite technique for gene expression studies, analyzing the etiology and disease progression, treatment effects, as well as designing the diagnostic ...many RNA ...
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Comparison of RNA extraction methods from biofilm samples of Staphylococcus epidermidis
... the RNA extraction seams to influence the total RNA quantity, as also described in the manufacturer ...the RNA yield is not linear and each kit normally has an optimal range of sample ...
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Comparison of commercial RNA extraction kits for preparation of DNA free total RNA from Salmonella cells
... Salmonella RNA extraction. The greatest concentration of total RNA was obtained with the MasterPure kit; however, the sample contained high levels of genomic DNA, which interferes with invA mRNA ...
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Comparison of RNA extraction kits and histological stains for laser capture microdissected prostate tissue
... of RNA isolated with the RNeasy (median RIN ...6.3) extraction kits, respectively ...quality RNA and led to suc- cessful RNA isolation of every ...isolate RNA from microdissected ...
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Rapid and Reliable Method of High Quality RNA Extraction from Diverse Plants
... quality RNA extraction is an important step for gene expression ...acid, RNA is used for protein synthesis, also widely employed in studies of gene expression pattern in different ...of RNA in ...
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Activated charcoal mediated RNA extraction method for Azadirachta indica and plants highly rich in polyphenolics, polysaccharides and other complex secondary compounds
... quality RNA is the primary requisite for the downstream applications and tools, but, its extrac- tion from many of the medicinal plants is tedious due to the presence of rich amount of polyphenols, polysaccha- ...
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RNA extraction method is crucial for human papillomavirus E6/E7 oncogenes detection
... different RNA extraction methods (Nuclisens manual extraction kit from bioMérieux, High Pure viral nucleic acid kit from Roche and RNeasy Plus Mini kit from Qiagen) and assess the difference found ...
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Evaluation of DNA and RNA Extraction Methods
... of RNA protocols, both methods in this study gave good result for the -actin gene ...usable RNA for PCR as the statistical analysis shows that Methods 1 and 2 are not significantly different (90% versus ...
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An Efficient Method for High Quality RNA Extraction from Moringa oleifera
... acid extraction from plants is diverse and varies among individual plant ...of RNA from some of the plant species may be problematic due to the presence of highly viscous polysaccharides and secondary ...
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An improved method for RNA extraction from woody legume species Acacia koa A. Gray and Leucaena leucocephala (Lam.) de Wit
... high-quality RNA of sufficient quantity from the stem tissues of tree legumes, such as Acacia koa and Leucaena leucocephala, because they contain high amounts of phenolic compounds and ...high-quality RNA ...
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Magnetic nanoparticles: a promising component in RNA extraction process
... Several RNA extraction procedures from various plant sources have been ...for RNA isolation are based on toxic organic solvents such as phenol or phenol/chloroform (17, 18, 19) that do not always ...
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The development of RNA extraction protocols to examine the effects of early exercise on gene expression in the articular cartilage and subchondral bone of Perendale sheep : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science at Massey University, Palmerston North, New Zealand
... isolated RNA from mineralized avian bone for Northern blot and in situ hybridization, by double extraction using cesium chloride density centrifugation and guanidine HCl-ethanol precipitation and hot phenol ...
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An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L
... total RNA extraction buffer {2% (w/v) CTAB (Sigma), 2% (w/v) polyvinylpyrrolidone (PVP-40) (Sigma), 100 mM Tris HCl (pH ...and RNA extraction in the ...onto RNA binding col- umns ...
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An improved method for the rapid isolation of RNA from Arabidopsis and seeds of other species high in polyphenols and polysaccharides
... Keywords: Arabidopsis thaliana, Brassica, Polysaccharide, RNA extraction, seeds... Financial support: This work was supported by a grant from the Biotechnology and.[r] ...
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Comparison of RNA isolation from FFPE tissue on two different platforms
... available RNA extraction kits for their ability to purify amplifiable RNA; the first one was manual platform using FormaPure kit, and another one was automated platform using SPRI-TE FFPE NA ...
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Cloning and functional analysis of LtuPTOX , a gene involved in carotenoid metabolism and orange band formation during tepal development in Liriodendron tulipifera
... Petals at various developmental stages, from S1 to S5, were used to determine the expression changes of the LtuPTOX gene. The five petal developmental stages are described in Fig. 5a: S1, female flower buds begin to ...
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83. Comparative profiling of gossypol enantiomers and seed traits’ related transcripts in cotton lines
... 22]. RNA quality and integrity is important in molecular analyses, such as RT-PCR, cDNA library construction, northern blotting and RNA sequencing ...for RNA extraction from plant tissues ...
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Applications of Competitor RNA in Diagnostic Reverse Transcription PCR
... of RNA viruses by reverse transcription (RT)-PCR has proven to be a useful approach for the diagnosis of infections caused by many viral ...competitor RNA can be used as a control for a number of different ...
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Multicenter Quality Assessment of PCR Methods for Detection of Enteroviruses
... Of the 14 laboratories that received the three quality assess- ment panels, 11 laboratories produced 13 datum sets for panel A and 11 laboratories produced 12 datum sets for panels B and C. Three data sets were generated ...
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