RNA isolation and cDNA library construction
Optimization and comparison of different methods for RNA isolation for cDNA library construction from the reindeer lichen Cladonia rangiferina
... conventional RNA extraction procedures and in the subsequent first strand cDNA ...standard RNA isolation/mRNA purification in cDNA library construction has resulted in ...
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An improved method for RNA isolation and cDNA library construction from immature seeds of Jatropha curcas L
... improve RNA extraction from various plant ...for RNA precipitation which was eliminated in the current method to reduce the time of RNA extraction from two days to 3 ...seed RNA (data not ...
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2 was GCGCGCAAGCTTCCNGTNGGNGAYGAYT- screen a HeLa cdna library for isolation of the full-length
... Reconstitution of p53-ubiquitinylation reactions from purified components: The role of human ubiquitin-conjugating enzyme UBC4 and E6-associated protein (E6AP).. (human papilloma virus/c[r] ...
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Construction and analysis of cotton (Gossypium arboreumL ) drought related cDNA library
... subtracted cDNA library is obtained, it is impor- tant to confirm that individual clones indeed represent differentially expressed ...subtracted library helps to eliminate false positives and to save ...
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Construction of rubella virus genome-length cDNA clones and synthesis of infectious RNA transcripts.
... using RNA extracted from RUB-infected cells as a template and oligonucleotide 43 (complementary to nucleotides 33 to 49 of the genome) as a primer resulted in a sequencing ladder.. toppe[r] ...
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Construction of cDNA library and preliminary analysis of expressed sequence tags from Siberian tiger
... ESTs cDNA libraries are widely used to identify genes and splice variants, and as a physical resource for full-length clones ...SMART library provided a useful resource for the functional genomic research ...
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The isolation and characterization of a Norwalk virus specific cDNA
... Furthermore, RNA extracted from serial postinfection, but not preinfection, stools from three of five volunteers hybridized to a Norwalk virus cDNA ...
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Isolation, identification, and partial cDNA cloning of genomic RNA of jaagsiekte retrovirus, the etiological agent of sheep pulmonary adenomatosis.
... Western blot analysis of gradient fractions when Freon- extracted sheep lung fluid (containing both JSRV and LV) was. separated to isopycnic equilibrium (Fig[r] ...
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The impact of RNA sequence library construction protocols on transcriptomic profiling of leukemia
... quality RNA, had approxi- mately similar insert sizes, and contained roughly the same amount of different types of ...non-coding RNA landscapes of the leukemia ...
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Construction of a genome-length cDNA clone for human astrovirus serotype 1 and synthesis of infectious RNA transcripts.
... Genomic cDNA clones from which infectious RNA can be transcribed have already been described for numerous other RNA viruses (1, 17, 18, 22, ...the construction of such an ‘‘infectious ...
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An improved protocol for small RNA library construction using High Definition adapters
... However, cDNA libraries of sRNAs are biased for molecules that are ligated to adapters more or less efficiently than other ...the cDNA insert when HD adapters were used to replace the kit ...
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Construction of a Severe Acute Respiratory Syndrome Coronavirus Infectious cDNA Clone and a Replicon To Study Coronavirus RNA Synthesis
... infectious cDNA clone and a functional replicon of the severe acute respiratory syndrome coronavirus (SARS-CoV) Urbani strain as bacterial artificial chromosomes (BACs) is described in this ...viral RNA was ...
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The influence of RNA integrity, purity and cDNA labelling on glass slide cDNA microarray image quality
... Methods RNA Isolation and cDNA Synthesis – RNA Purity and Integrity The data used in our analysis was collected from a variety of microarray studies ongoing within our laboratory with the ...
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Original Article Construction of human colon cancer stem cells cDNA library and identification of novel proteins interacted with stratifin
... SMART cDNA Library Construction Kit was used to constructed cDNA library of human colon cancer stem cells and the quality of it was ...cells cDNA library ...cells ...
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Construction and Analysis of SSH cDNA Library from Leaves of Susceptible Rubber Clone Resistant to Powdery Mildew Induced by BTH
... expressed cDNA library was constructed by suppression subtractive hybridization (SSH) with rubber Reyan 7-33-97 ...constructed cDNA library was high integrity through detection of the critical ...
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Isolation of total RNA with the illustra RNAspin Mini Isolation Kit
... total RNA product in QRT-PCR A 100-mg aliquot of rat liver tissue was frozen and subsequently stored in RNAlater-ICE ...Total RNA was extracted using the RNAspin Mini RNA Isolation Kit and the ...
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Castor bean lectins : construction and sequencing of cDNA clones
... A recognised problem with M13 cloning is the risk of generating clones with multiple inserts (235), this occurring during ligation of the mixture of target fragments with the vector. This is not usually a serious problem ...
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Construction and characterization of an infectious cDNA clone of coxsackievirus A 10
... Methods: The whole genome of CA10 derived from a clinical isolate was amplified into two fragments and ligated into a linearized plasmid vector in one step by In-Fusion Cloning. The obtained CA10 cDNA clones and ...
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Construction of a kiwifruit yeast two hybrid cDNA library to identify host targets of the Pseudomonas syringae pv actinidiae effector AvrPto5
... Figures S3, S4). As a result, the medium SDA/-Leu-Trp- His could be employed as a minimal medium to monitor Psa AvrPto5 interactions. After screening the library, 53 positive interactions were identified. The prey ...
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Construction of a GAL1-regulated yeast cDNA expression library and its application to the identification of genes whose overexpression causes lethality in yeast.
... Among 15 clones that showed galactose inducible lethality were cDNAs encoding structural proteins, including ACTl (actin), TUB2 (&tubulin) and ABPl (actin- binding [r] ...
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