RNA stability
Phosphorylation of Measles Virus Nucleoprotein Affects Viral Growth by Changing Gene Expression and Genomic RNA Stability
... viral RNA genome to form the N-RNA complex, providing a template for viral RNA ...lower stability against RNase and faster turnover of viral genomic ...
9
Reovirus Nonstructural Protein σNS Acts as an RNA Stability Factor Promoting Viral Genome Replication
... an RNA stability factor increasing the half-life of viral ...in RNA binding because it lacks a series of positively charged residues predicted to be required for that activity (51, ...increases ...
23
RBP38, a Novel RNA-Binding Protein from Trypanosomatid Mitochondria, Modulates RNA Stability
... into RNA by any known posttranscriptional processing ...or RNA turnover and that the results from those experiments were not superimposed with results from any other RNA- processing event such as ...
9
Multicenter Evaluation of Use of Dried Blood and Plasma Spot Specimens in Quantitative Assays for Human Immunodeficiency Virus RNA: Measurement, Precision, and RNA Stability
... the RNA in the blood and plasma spots. HIV RNA was eluted from the filter paper by treatment with a chaotrope, organic reagent, and detergent (CORD) solution consisting of 33% (vol/vol) buffered phenol (pH ...
6
Regulation of RNA stability by terminal nucleotidyltransferases
... activity, RNA binding affinity was unperturbed, suggesting Gld2 phosphorylated at S116 binds the RNA substrate in a non-productive ...different RNA binding modes. The wildtype Gld2 enzyme binds ...
185
Temporal and spacial control of RNA stability in the early embryo of Drosophila melanogaster
... In addition, oskar and Pgc transcripts move back to the posterior pole of the oocyte at stage 9, whereas the bicoid and Add-hts RNAs never show the early posterior localization but are e[r] ...
97
Structural features in the Rous sarcoma virus RNA stability element are necessary for sensing the correct termination codon
... full-length RNA is presented to the host translation machinery with characteristics rarely observed among host cell mRNAs: a long 3′ UTR, retained introns, and multiple open reading ...viral RNA is ...
15
Distinct RNA sequences in the gag region of human immunodeficiency virus type 1 decrease RNA stability and inhibit expression in the absence of Rev protein.
... In conclusion, we present evidence arguing that unspliced HIV-1 mRNAs contain distinct sequences other than splice sites which decrease the stability of the viral mRNAs and inhibit the e[r] ...
10
Structural Characterization of the Rous Sarcoma Virus RNA Stability Element
... retroviral RNA escapes this ...(RSV) stability element (RSE), an RNA element downstream of the gag natural translation termination codon that prevents degradation of the unspliced viral ...
11
Interaction between Brome Mosaic Virus Proteins and RNAs: Effects on RNA Replication, Protein Expression, and RNA Stability
... The RNA silencing assay and plasmids of Johansen and Carrington (41) were adapted to detect the reduction of the signal from GFP in Agrobacterium- infiltrated tobacco ...BMV RNA levels in transfected ...The ...
13
Effects of specimen collection, processing, and storage conditions on stability of human immunodeficiency virus type 1 RNA levels in plasma
... HIV-1 RNA levels were quantitated with the NASBA HIV-1 RNA QT System from blood specimens that were collected, processed, and stored under a variety of conditions that might have affected HIV-1 RNA ...
8
Exploring the stability of long intergenic non-coding RNA in K562 cells by comparative studies of RNA-Seq datasets
... that RNA stability influences the abundance of transcript and shapes the kinetics of gene induction in intricate gene networks in mammalian cells ...lincRNA stability inevitably affects its function ...
15
Use of External Quality Control Material for HIV 1 RNA Testing To Assess the Comparability of Data Generated in Separate Laboratories and the Stability of HIV 1 RNA in Samples after Prolonged Storage
... HIV-1 RNA cutoffs for evaluating virologic failure across HIV-1 RNA assay platforms ...HIV-1 RNA assays, the Cobas, Roche Cobas AmpliPrep Cobas TaqMan, and Abbott RealTime HIV-1 RNA assays, ...
10
Stability of tandem repeats in the Drosophila melanogaster Hsr-omega nuclear RNA.
... The sequences of the regions immedi- ately flanking these monomers on either end of the repeat region show less than one monomer length of irregular sequence with s[r] ...
11
Circular RNA CDR1as sponges miR-7-5p to enhance E2F3 stability and promote the growth of nasopharyngeal carcinoma
... Total RNA was extracted from tissues and cells using Trizol reagent (Invitrogen, 15596026, Fisher Scientific, USA), and the specific steps were strictly carried out according to the instructions of the ...total ...
13
Pleiotropic Effects of Resistance-Breaking Mutations on Particle Stability Provide Insight into Life History Evolution of a Plant RNA Virus
... of the within-host multiplication component of virus fitness (11–17). In order to fully understand the evolution of resistance breaking, it is necessary to consider potential costs on other fitness components, as the ...
17
PTEN expression is upregulated by a RNA-binding protein RBM38 via enhancing its mRNA stability in breast cancer
... PTEN. RNA immunoprecipitation, RNA electrophoretic mobility shift and dual-luciferase reporter assays were employed to identify the direct binding sites of RBM38 with PTEN ...
11
Interactome Analysis of the Human Respiratory Syncytial Virus RNA Polymerase Complex Identifies Protein Chaperones as Important Cofactors That Promote L-Protein Stability and RNA Synthesis
... The ablation of viral gene expression in infected cells treated with 17-AAG may have been due to an overall decrease in stability of all viral proteins or, specifically, one or more of the proteins involved in ...
14
De Novo Initiation of RNA Synthesis by the Arterivirus RNA-Dependent RNA Polymerase
... initiated RNA synthesis de novo on homopolymeric templates in a template-specific ...novo RNA synthesis generally initiates with a purine as the starting ...of RNA synthesis or runoff polymerization ...
12
Regional Stability and Stability Radius*
... Abstract. In this work we consider the problem of stability, for distributed parameter systems, through the space variable. We give an extension of the stability radius, introduced by A. J. Pritchard and S. ...
14