Rolling Circle Amplification
A novel method for detection of HBVcccDNA in hepatocytes using rolling circle amplification combined with in situ PCR
... nonexponential rolling circle amplification ...less amplification bias and greater yield, product length, and fidelity than conventional PCR ...efficient amplification was operated by ...
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Rolling Circle Amplification for Direct Detection of rpoB Gene Mutations in Mycobacterium tuberculosis Isolates from Clinical Specimens
... tions, including single-nucleotide polymorphisms (SNPs); liga- tion of the probe ends by DNA ligase requires perfectly matched target-probe complexes, which prevents the nonspecific amplifi- cation that can occur with ...
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Rolling Circle Amplification Is More Sensitive than PCR and Serology Based Methods in Detection of Banana streak virus in Musa Germplasm
... techniques: rolling circle amplification (RCA), immunocapture PCR (with degenerate and Gold finger primers) and standard PCR was ...the amplification of the integrated nuclear viral sequences, ...
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Characterization of Two Novel Polyomaviruses of Birds by Using Multiply Primed Rolling-Circle Amplification of Their Genomes
... Polyomaviruses are small nonenveloped particles with a circular double-stranded genome, approxi- mately 5 kbp in size. The mammalian polyomaviruses mainly cause persistent subclinical infections in their natural ...
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Rapid and Sensitive Detection of Severe Acute Respiratory Syndrome Coronavirus by Rolling Circle Amplification
... The severe acute respiratory syndrome (SARS) epidemic of 2003 was responsible for 774 deaths and caused significant economic damage worldwide. Since July 2003, a number of SARS cases have occurred in China, raising the ...
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Identification and Typing of Isolates of Cyphellophora and Relatives by Use of Amplified Fragment Length Polymorphism and Rolling Circle Amplification
... The species diversity and identification of black fungi belonging to Cyphellophora and Phialophora, which colonize and infect human skin and nails, were studied using amplified fragment length polymorphism (AFLP). A ...
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From Interface to Solution: Integrating Immunoassay with Netlike Rolling Circle Amplification for Ultrasensitive Detection of Tumor Biomarker
... (netlike rolling circle amplification) strategy, which integrates immunoassay with NRCA for the ultrasensitive detection of tumor biomarker, by taking the assay of a tumour marker as an ...signal ...
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In Situ Detection of Anaplasma spp by DNA Target Primed Rolling Circle Amplification of a Padlock Probe and Intracellular Colocalization with Immunofluorescently Labeled Host Cell von Willebrand Factor
... Transformation of A. phagocytophilum that renders it able to express green fluorescent protein and subsequent experimental infection of mice with A. phagocytophilum transformants have been recently described (6). Such ...
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Development of an in situ assay for simultaneous detection of the genomic and replicative form of PCV2 using padlock probes and rolling circle amplification
... localised rolling circle amplification (RCA) [2], giving rise to long single- stranded DNA molecules that spontaneously coil into ~1 μm sized objects that can easily be observed as dis- crete bright ...
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Rapid Identification and Differentiation of Trichophyton Species, Based on Sequence Polymorphisms of the Ribosomal Internal Transcribed Spacer Regions, by Rolling Circle Amplification
... and rolling-circle amplification (RCA)-based method for identification of single nucleotide polymorphisms (SNPs) that could be exploited to differentiate between Trichophyton ...
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Practical Method for Detection and Identification of Candida, Aspergillus, and Scedosporium spp by Use of Rolling Circle Amplification
... Recently, the utility of circularizable oligonucleotide (padlock) probes has been demonstrated for the detection of target nucleic acid sequences, including nucleotide polymorphisms that differ by only a few base pairs, ...
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Characterization of a Novel Close-to-Root Papillomavirus from a Florida Manatee by Using Multiply Primed Rolling-Circle Amplification: Trichechus manatus latirostris Papillomavirus Type 1
... [pH 8.5], with 1 l of 10-fold-diluted RCA product of the manatee sample or of water (negative control) as the template. PCR conditions were 10 min of denaturation at 94°C; 45 cycles of 1 min at 94°C, 1 min at 50°C, and ...
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An Impedimetric Sensor for Real-Time Detection of Antibiotic Resistance Genes Employing Rolling Circle Amplification
... detection methods involving complex architectures and relatively expensive instrumentation. Because of their simplicity, and especially in view of the demands for an increased level of system integration in future POC ...
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Rolling Circle Amplification on Biotin Streptavidin Complexes Immobilized to Activated Cyclic Polyolefin Surfaces
... croarrays [12]. A commonly used COP surface treatment is UV-irradiation grafting of a benzophenone (BP)/acryl amide or methylacrylate [3,6,8,16,17]. Nexus Biosystems (San Diego CA) sells COP microtiter plates (Aurora Mi- ...
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Colorimetric Nucleic Acid Testing Assay for RNA Virus Detection Based on Circle to Circle Amplification of Padlock Probes
... and circle-to-circle amplification (C2CA) technology (4) combined with enzymatic readout has been developed ...exponential rolling-circle amplification (RCA)-based C2CA reaction ...
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Characterization of Adeno-Associated Virus Genomes Isolated from Human Tissues
... Linear rolling-circle amplification of episomal ...linear rolling-circle amplification to specifi- cally amplify double-stranded, circular AAV genomes from total cellular ...
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Whole genome sequencing of enriched chloroplast DNA using the Illumina GAII platform
... Multiply-primed rolling circle amplification (RCA) was used to produce an abundance of purified chloroplast DNA template in preparation for sequencing ...of amplification bias making the ...
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Three Isothermal Amplification Techniques for Rapid Identification of Cladophialophora carrionii, an Agent of Human Chromoblastomycosis
... isothermal amplification tech- niques, which amplify DNA by strand replacement without using a thermocycler, have become ...isothermal amplification (LAMP) assays for the rapid diagnosis of Talaromyces ...
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(HOMOPTERA; PLANOCOCCIDAE) AS A VECTOR OF Paracoccus burnerae Banana streak virus
... 2.1 Screening of virus-source plants and receptor plants BSV-infected banana plantlets were obtained from infected banana germplasm materials at Kenya Agricultural Research Institute (KARI), Njoro greenhouse and ...
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Metagenomic Discovery of 83 New Human Papillomavirus Types in Patients with Immunodeficiency
... enrichment, rolling circle amplification, and deep sequencing to identify circular DNA viruses present in skin swabs and/or wart biopsy samples from 48 patients with rare genetic immunodeficiencies, including ...
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