RT-PCR, reverse transcriptase–polymerase chain reaction
A comparative analysis of routine techniques: Reverse transcriptase polymerase chain reaction (RT-PCR) and five cell lines for detection of enteroviruses in stool specimens
... detection. RT-PCR (Reverse Transcriptase Polymerase Chain Reaction) is a technique used in place of the cell culture ...with RT-PCR for detection of ...
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Gene expression induced by interleukin 17 in fibroblast like synoviocytes of patients with rheumatoid arthritis: upregulation of hyaluronan binding protein TSG 6
... kinase; RT–PCR = reverse transcriptase– polymerase chain reaction; RA = rheumatoid arthritis; SFC = fibroblast-like synoviocytes; TBST = tris-buffered saline containing ...
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Heminested reverse transcriptase polymerase chain reaction (hnRT PCR) as a tool for rabies virus detection in stored and decomposed samples
... rogen) and ultra pure water in a final volume of 50 μL. The amplification was performed on a MJ Research PCT- 200 Thermal Cycler. The following cycling conditions were adopted: initial heating at 95°C/3 min, 35 cycles at ...
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Eye Findings in Infants With Suspected or Confirmed Antenatal Zika Virus Exposure
... with reverse transcriptase polymerase chain reaction (RT-PCR)– positive and unconfirmed case patients who were born during and soon after an outbreak period is used to ...
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COMPARING THE GENES OF Artemisia annua AND Artemisia ludoviciana WITH RESPECT TO THE ARTEMISININ BIOSYNTHETIC PATHWAY
... the reverse transcriptase polymerase chain reaction (RT-PCR) were subjected to an agarose gel electrophoresis to visualize amplicons obtained against a molecular weight ...
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Magnetic Nanowire Networks for Dual-Isolation and Detection of Tumor-Associated Circulating Biomarkers
... by reverse transcriptase polymerase chain reaction (RT-PCR) and quantitative real-time PCR (qPCR) (Figure ...
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MOLECULAR ANALYSIS OF MLL GENE REARRANGEMENTS IN CHILDHOOD ACUTE LEUKEMIA: A HOSPITAL BASE STUDY
... multiplex reverse-transcriptase polymerase chain reaction (PMRT-PCR) technique was performed for molecular ...Quantitative RT-PCR assay of MLL revealed a down ...
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A report on Japanese encephalitis in Mizoram: clinical investigation and diagnosis
... However, reverse transcriptase polymerase chain reaction (RT-PCR) based methodologies have resulted in increased sensitivity for the detection of JEV viruses in clinical ...
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Application of in situ reverse trancriptase-polymerase chain reaction (RT-PCR) to tissue microarrays
... quantitative RT-PCR analysis of gene expression [13,14] was carried out on first-strand cDNA derived from RNA isolated from samples of normal and tumour ...Each PCR reaction contained 10 ng ...
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Multiplex reverse transcriptase pcr assay for the detection of infective stage wuchereria bancrofti and its vector culex quinquefasciatus
... multiplex reverse transcription polymerase chain reaction (RT-PCR) assay for the simultaneous and the vector species Culex ...
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Epidemiological and Genetic Overview of the Klebsiella pneumoniae Carbapenemases (KPCs) in K. pneumoniae Isolated from the Clinical Samples in Iran
... Methods: One hundred and eighty-one K. pneumoniae strains were collected from patients presenting to Firoozgar Hospital of Tehran, Iran. The strains were tested using the disk diffusion method, the modified Hodge test ...
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Osteoblastic differentiation and stress response of human mesenchymal stem cells exposed to alternating current electric fields
... Universal PCR Master Mix (Applied Biosystems, Foster City, CA) and the ABI Prism 7000 Sequence Detection System (Applied ...Biosystems). RT-PCR deter- mined mRNA expression of osteogenic ...
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Skeletal muscle expression of creatine kinase-B in end-stage renal disease
... Taq Polymerase solution, 5 U/µL (Promega Corporation); 2 µL forward primer and reverse primer solutions both at 15 µM; 2 µL of reverse-transcribed cDNAs; and ...final reaction volume of 50 ...
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Novel PCR-ELISA Technique as a Good Substitute in Molecular Assay
... of PCR-ELISA and its applications PCR-ELISA is containing of PCR and ELISA techniques that allows to detection of nucleic acid instead of pro- ...the PCR product directly after immobilization ...
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Identification and characterization of a novel efficient resistance response to the furoviruses SBWMV and SBCMV in barley
... The combined natural and artificial virus inoculation datasets suggest one or more of the following mechanisms for extreme resistance to furoviruses in barley: i) complete prevention of virus replication and spread in ...
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Microarray profile of differentially expressed genes in a monkey model of allergic asthma
... real-time RT-PCR (Taqman). The gene-expression levels obtained by RT-PCR were normalized to that of gene for the enzyme hypoxanthine-guanine phos- phoribosyl transferase (HPRT) and expressed ...
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A New Sensitive Method for Detection of Viroids
... acid. RT-PCR-DBH assay for viroid detection have two levels of specificity based on RT-PCR primers and an internal hybridization ...probe. RT-PCR-DBH assay with the specificity, ...
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Cloning of a muscle specific calpain from the American lobster Homarus americanus: expression associated with muscle atrophy and restoration during moulting
... Here we report the cloning of a crustacean calpain gene with features that distinguish it from calpains isolated from other species. The cDNA encodes a non-EF-hand calpain with a unique N-terminal sequence and an ...
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Altered serum microRNAs as biomarkers for the early diagnosis of pulmonary tuberculosis infection
... Real-time qRT-PCR was performed in individual serum samples to further verify the ten candidate miRNAs iden- tified by TLDA. Differential expression of seven miRNAs (miR-361-5p, miR-889, miR-576-3p, miR-210, ...
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The influence of micrometastases on prognosis and survival in stage I-II colon cancer patients: the Enroute⊕ Study
... [3-5] Focused examination methods, such as more extensive nodal examination by serial sectioning or step sectioning, or molecular detection of metastatic nodal cells by immunohistochemistry (IHC) or reverse tran- ...
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