Sample preparation in mass spectrometry
Comparison of Sample Preparation Methods, Instrumentation Platforms, and Contemporary Commercial Databases for Identification of Clinically Relevant Mycobacteria by Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry
... flight mass spectrometry (MALDI-TOF MS) has recently been reported to be a reliable and expedited method for identifica- tion of mycobacteria, although various specimen preparation techniques and ...
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Simplified Plant Sample Preparation for use in Gas Chromatography-Mass Spectrometry (GC-MS) Based Metabolomic Profiling and Targeted Analyte Quantitation
... laborious sample preparation protocol was developed for the analysis of Arabidopsis thaliana using Gas chromatography coupled with mass spectrometry ...simplified sample ...
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Biological tissue sample preparation for time-of-flight secondary ion mass spectrometry (ToF–SIMS) imaging
... ion mass spectrometry (ToF–SIMS) imaging is an analytical technique rapidly expanding in use in biological ...In mass spectrometry imaging (MSI), sample preparation is a crucial ...
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Evaluation of sample preparation methods for mass spectrometry-based proteomic analysis of barley leaves
... After protein digestion, peptides (~ 20 μg) were desalted using Poros ® 20 R2 reversed phase microcolumns as pre- viously described [31]. Dried peptides were dissolved in mobile phase A (0.1% formic acid) and the ...
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A SELDI mass spectrometry study of experimental autoimmune encephalomyelitis: sample preparation, reproducibility, and differential protein expression patterns
... ratio is consistent with it being a proteolytic fragment of MBP. The reduction of the 14.2 kDa MBP isoform and the increase in the 12.4 kDa MBP fragment in EAE ani- mals with stage 3 diseases is clearly evident in the ...
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The development of a method for chloramphenicol determination in meat products
... of sample preparation for analysis. Sample preparation affects all subsequent steps of analysis of the antibiotic, and is therefore crucial to the absolute identification, confirmation and ...
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Electric field driven sample clean up strategies for mass spectrometry applications
... in sample preparation and has been thoroughly discussed in numerous reviews 50-52 ...aqueous sample solution is passed through an immobilized phase and then the retained analytes are eluted with an ...
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Applications of Mass Spectrometry to Analysis of Prodiginines, Bioactivated Methylenedianiline Intermediates, and Hypoxia Induced Changes in the Zebrafish Skeletal Muscle Proteome
... 45 mass spectrometry earned the reputation for its high sensitivity, broad dynamic range of measurements, rich information obtained from complementary fragmentation techniques like CID, 45, 46 infrared ...
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A modified FASP protocol for high-throughput preparation of protein samples for mass spectrometry
... the preparation of protein samples for mass spectrometry, the original FASP methodology used individual ultrafiltration devices, such as Microcon (Milli- pore) or Vivacon (Sartorius-Stedim), that are ...
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Dispersive liquid-liquid microextraction-gas chromatography-mass spectrometric determination of polycylic aromatic hydrocarbons in water
... a sample preparation method based on dispersive liquid-liquid microextraction (DLLME) coupled with gas chromatography-mass spectrometry (GC-MS) was developed for the extraction of polycyclic ...
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Preparation of flaxseed for lignan determination by gas chromatography–mass spectrometry method
... Sample preparation. Prior to the sample prepa- ration, all glassware was silanised in a 5% solution of dimethyldichlorosilane in heptane, followed by deactivation of excess reagent in ...
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DEVELOPMENT AND VALIDATION OF METHAMPHETAMINE ANALYSIS METHODS IN DRIED BLOOD SPOT SAMPLE USING GAS CHROMATOGRAPHY – MASS SPECTROMETRY
... the sample. Preparation was carried out by adding methamphetamine with a concentration of 1000 ng / mL as much as 40 μL into the blood with a 43% hematocrit of 360 μL so that the concentration of analyte ...
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Fast Determination of 22 Pesticides in Rice Wine by Dispersive Solid Phase Extraction in Combination with GC MS
... [16] Y. Jiang, X. Li, J. Xu, C. Pan, J. Zhang and W. Niu, “Multiresidue Method for the Determination of 77 Pes- ticides in Wine Using QuEChERS Sample Preparation and Gas Chromatography with Mass ...
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Optimized sampling protocol for mass spectrometry-based metabolomics in Streptomyces
... The sample preparation procedure customized for the study of Streptomyces metabolomics is so rare that most researchers have referenced the procedure for other bac- teria (Lu et ...for sample ...
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Endogenous expression mapping of malignant melanoma by mass spectrometry imaging
... a sample amena- ble to analysis by MALDI-MS ...manual preparation methods that were based on a thin-film technique that was generated using a multi-layer ...a mass spectrometry (MALDI) ...
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Lipidomic analysis of bloodstream and procyclic form Trypanosoma brucei
... MS technology adapted to nano fl ow mode has made it possible to e ffi ciently detect all the major T. brucei PLs in one liquid sample of total lipid extract. Overall, the results presented here for the composition ...
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Review: Isomer Separation, Chiral Resolution, and Structure Elucidation Analyses Are the Future of Analytical Supercritical Fluid Chromatography—Mass Spectrometry
... process of CCSFC-MS was not much higher than that in GC-MS. Some signs of chemical ionization pheno- mena could be observed in the mass ions, additive ions and fragmentation patterns; however, this occurred to a ...
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Trace element analysis of whole-rock glass beads of geological reference materials by Nd:YAG UV 213 nm LA-ICP-MS
... Si are respectively used as the external standard and the internal standard element, the GRM beads yield trace element compositions listed in Table 4. The NIST612 glass was analyzed in triplicate for each sample ...
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Identification of N Methyl Bis(2 (Alkyloxy Alkylphosphoryloxy)Ethyl) Amines by LC HRMS/MS
... MS analyses were performed using an Agilent 6520 quadruple-time-of-flight mass spectrometer fitted with an electrospray (ESI) interface, operated in positive ESI mode. Capillary voltage was set at 3.5 kV, ...
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Trends in cotinine level in urine samples of smokeless tobacco chewers from Belagavi region
... Cotinine was quantitatively estimated using RP-HPLC method and the results were expressed in ng/mL urine sample. Clear and sharp peaks for standards (Figure 1a) and urine samples (Figure 1b) were obtained during ...
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