SE gene identification using multiplex PCR
Development of a Multiplex PCR for Identification of Vineyard Mealybugs
... mitochondrial gene cytochrome coxidase subunit I (COI) to distin- guish populations of ...species using only one PCR reaction but added a post-PCR step to the pro- ...tocol. Multiplex ...
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Rapid Identification of Pathogenic Fungi Directly from Cultures by Using Multiplex PCR
... A multiplex PCR method was developed to identify simultaneously multiple fungal pathogens in a single ...rRNA gene to identify Candida albicans, Candida glabrata, Candida parapsilosis, Candida ...
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Primer Design for the Identification of Ten Oral Actinomyces Species Using Multiplex PCR
... species identification using PCR [16]. Moreover, multiplex-PCR is a rapid tool that allows for the simultaneous amplification of more than one sequence of target DNA in a single ...
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Species Specific Identification of Human Adenoviruses by a Multiplex PCR Assay
... a multiplex PCR assay that could supplant hemagglutination for Ad species ...Whereas PCR assays described for identification of Ad species and serotypes have targeted the hexon (42), pIX (3), ...
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Multiplex Real Time PCR Targeting the RNase P RNA Gene for Detection and Identification of Candida Species in Blood
... PCR with primers in regions CR-I and CR-V and C. glabrata DNA as a template did not produce any amplicon of the expected size. The product was instead over 1,000 bp and was at first thought to be unspecific. ...
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Virus detection and identification using random multiplex (RT) PCR with 3' locked random primers
... However, both of these technologies rely on a relatively stable genome, and several human pathogens display a high mutation rate (e.g., HIV 2 × 10 -5 /base, 9 kB genome [5]). Moreover, the ability to recombine ...
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Rapid identification of pathogens involved in pediatric osteoarticular infections by multiplex PCR
... either using 16S rDNA PCR or conventional PCR targeting the specific gene cpn60 of ...the multiplex PCR assay in the present study is designed as a flexible system in which the ...
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Identification of Vibrio Isolates by a Multiplex PCR Assay and rpoB Sequence Determination
... approach using rpoB sequencing and phylogenetic analysis that can cluster isolates into other Vibrio species and can clearly differentiate Vibrio from other closely related genera, such as ...The multiplex ...
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Use of the flagellar H7 gene as a target in multiplex PCR assays and improved specificity in identification of enterohemorrhagic Escherichia coli strains
... eaeA PCR assay allowed for the detection of all (26 of 26) class I EPEC strains, and among 47 EHEC strains positive by the fluores- cence actin staining test, 45 were also positive by the eaeA ...serogroup ...
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Salmonella Serovar Identification Using PCR Based Detection of Gene Presence and Absence
... individual PCR primer may fail due to a previously unknown single base polymorphism in the primer binding ...real-time PCR and multiplex ...other PCR products in the multiplex which are ...
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Multiplex PCR for Simultaneous Identification ofStaphylococcus aureus and Detection of Methicillin and Mupirocin Resistance
... therefore, PCR assays have become an essential tool in laboratory ...of PCR for the accurate detection of the mecA gene (23, 27) and the possibility of simultaneous identi- fication of ...ileS-2 ...
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Use of the omp50 Gene for Identification of Campylobacter Species by PCR
... A PCR assay was developed, using the omp50 gene as a species-specific ...samples. Multiplex PCRs have been developed to identify ...that identification of ...
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Cryptococcus species identification by multiplex PCR
... Serotyping using the Crypto Check kit was used as a gold standard in this ...The multiplex PCR identifications were in 100% agreement with those obtained through ...The PCR was more accurate ...
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Troubleshooting for PCR and multiplex PCR
... (multiplexed) locus individually, using an annealing temperature lower than usual. Compare the unspecific products for each locus tested with the unspecific products seen when running the multiplex ...
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Multiplex PCR for identification of methicillin resistant staphylococci in the clinical laboratory
... A multiplex PCR assay for detection of the staphylococcal mecA gene (the structural gene for penicillin- binding protein 2a) was compared with agar dilution and disk diffusion susceptibi[r] ...
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Multiplex PCR Method for Species Identification of Coagulase Positive Staphylococci
... In veterinary medicine, coagulase-positive staphylococci (CoPS) other than Staphylococcus aureus have frequently been misidentified as being S. aureus strains, as they have several phenotypic traits in common. There has ...
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Detection and Identification ofActinobacillus pleuropneumoniae Serotype 5 by Multiplex PCR
... a multiplex PCR assay was developed for detection of ...and identification of serotype 5 ...The multiplex PCR assay enabled us to detect ...
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Identification of Bacillus anthracis by rpoB Sequence Analysis and Multiplex PCR
... precise identification of ...definitive identification of ...laboratories. PCR can be used as a preliminary method for detecting ...Moreover, PCR may be extended to the large-scale exposure ...
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Use of a Genus and Species Specific Multiplex PCR for Identification of Enterococci
... when using the BBL Crystal ID kit for identification ...the multiplex PCR was designed to amplify specific regions of enterococcal species, this property can be both a positive and a negative ...
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A multiplex PCR for rapid identification of Brassica species in the triangle of U
... separated by 30 kbp), and two C genome specific markers, C1 (157 bp, located on chromosome C01 of B. oleracea) and C9 (612 bp, located on chromosome C09 of B. olera- cea) were obtained. Primers for the A, B (331 bp) [20] ...
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