Self cleavage
Circularity and self-cleavage as a strategy for the emergence of a chromosome in the RNA-based protocell
... small, self-cleaving ribozymes ...and self-cleavage are important for the spread of the ...and self-cleavage may have been adopted as a strategy to overcome the immediate difficulties ...
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Expression of hepatitis delta virus RNA deletions: cis and trans requirements for self-cleavage, ligation, and RNA packaging.
... RNAs from within cells, as well as those secreted in the presence of HBV sAg-S, were then evaluated by Northern analysis to assess their ability to undergo self-cleavage, ligation, and i[r] ...
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Antigenomic RNA of human hepatitis delta virus can undergo self-cleavage.
... In partial confirmation of this hypothesis, we found that in vitro the RNA complementary to the HDV genome, the antigenomic RNA, could undergo a self-cleavage that was not only more than[r] ...
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RNase footprinting demonstrates antigenomic hepatitis delta virus ribozyme structural rearrangement as a result of self cleavage reaction
... ribozyme self-cleavage also exhibits one of the two alternative states for the region between U15 and ...SBL self-cleavage possesses all elements included in the pseudoknot ...
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The Processing of eIF4GI by Human Rhinovirus Type 2 2Apro: Relationship to Self-Cleavage and Role of Zinc
... A crucial step in the life cycle of many viruses is the proteo- lytic processing of viral and cellular proteins (6). This is espe- cially evident with certain picornaviruses which encode pro- teinases which not only ...
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Evidence for intramolecular self-cleavage of picornaviral replicase precursors.
... RUECKERT Biophysics Laboratory of the Graduate School and Department of Biochemistry of the School of Agriculture and Life Sciences, University of Wisconsin, Madison, Wisconsin 53706 It [r] ...
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Non-Canonical Functions Of The Bacterial Sos Response
... LexA self-cleavage activity, our lab previously performed extensive amino acid profiling of the cleavage site loop of LexA from ...determined cleavage profiles for each position (Mo, ...LexA ...
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Natural 2′,5′-Phosphodiester Bonds Found at the Ligation Sites of Peach Latent Mosaic Viroid
... to self-ligate in vitro primarily via the formation of 2⬘,5⬘-phosphodiester bonds ...prevent self-cleavage of the replicational circular templates, resulting in a significant advantage in terms of ...
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Characterization of self-cleaving RNA sequences on the genome and antigenome of human hepatitis delta virus.
... In our previous RNA of synthesized Self-cleavage study 14 we were able to locate the unique self-cleavage site on the antigenomic RNA of HDV, synthesized in vitro from cloned DNA, by use[r] ...
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A Synthetic Circuit For Control Of The Bacterial Dna Damage Response Without Dna Damage
... To obtain orthogonal control over the SOS pathway, we envisioned engineering an E. coli LexA variant that could be rendered dysfunctional inside the cell, not by self- cleavage but by an alternative ...
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Cleavage of the C-Terminal Fragment of Reovirus μ1 Is Required for Optimal Infectivity
... Recombinant reovirus with mutation 1 Y581A. Reovirus entry is characterized by a series of stepwise disassembly events. During virion-to-ISVP conversion, the 3 protein is degraded and the membrane penetration ...
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Specific proteolytic cleavage of recombinant Norwalk virus capsid protein.
... a cleavage prod- uct of the NV capsid ...the cleavage product obtained by trypsin digestion of rNV capsid protein, we hypothesized that this cleavage product might be the source of the 30K soluble ...
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Proteolytic processing of imported chloroplast proteins
... that cleavage was markedly improved when the processing peptidases were present in the translation ...single cleavage product was observed with each ...
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The early development of the onychopod cladoceran Bythotrephes longimanus (Crustacea, Branchiopoda)
... cytoplasmic cleavage is still not complete and the resulting eight blastomere compartments are still connected in the center (Figure 3L, M, ...This cleavage is perpendicular to the previous divisions and ...
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Low Endocytic pH and Capsid Protein Autocleavage Are Critical Components of Flock House Virus Cell Entry
... While biochemical and structural studies have provided the foundation for a model of FHV cell entry, more rigorous in vivo and in vitro studies are necessary to confirm the ideas put forth in this model. Here, we clarify ...
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Role of Calpain in the Formation of Human Papillomavirus Type 16 E1^E4 Amyloid Fibers and Reorganization of the Keratin Network
... calpain cleavage of 16E1 E4, this process can be ...of cleavage to completely prevent reorganization since some degree of reorganization would also be expected as a result of monomeric 16E1 E4 binding, ...
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Molluscum Contagiosum Virus Topoisomerase: Purification, Activities, and Response to Inhibitors
... FIG. 1. Analysis of MCV topoisomerase proteins by SDS-PAGE. Lanes: 1, size markers; 2, purified (HT)MCV-TOP; 3, MCV-TOP (the same fractions as in lane 2 but lacking the amino-terminal hexahistidine tag which was removed ...
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Biochemical investigations of the carotenoid cleavage dioxygenase enzyme family
... Page | xiii cleavage of 9’-cis-neoxanthin in the absence (middle, B, 71% conversion) and presence (bottom, C, 50% conversion) of 100 µM D30. Additional peaks in chromatograph are believed to be other carotenoids ...
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Targeting the membrane-anchored serine protease testisin with a novel engineered anthrax toxin prodrug to kill tumor cells and reduce tumor burden
... activation cleavage of PrAg-PCIS by both recombinant matriptase and hepsin in vitro suggested that the full-length forms of these membrane-tethered enzymes could be additional activators of ...activation ...
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Genetic Predisposition To Acquire a Polybasic Cleavage Site for Highly Pathogenic Avian Influenza Virus Hemagglutinin
... Accelerated nucleotide insertions in the presence of consecutive adenine residues. We then hypothesized that a stretch of adenine residues in the RNAseqHAclv region might affect the frequency of nucleotide insertions, as ...
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