Semi-Quantitative RT-PCR
A semi-quantitative RT-PCR method to measure thein vivo effect of dietary conjugated linoleic acid on porcine muscle PPAR gene expression
... a semi-quantitative RT-PCR method was developed using cDNA normalized against the housekeeping genes cyclophilin and β- ...expensive PCR instruments with real-time fluorescent ...
9
Measurement of the Infection and Dissemination of Bluetongue Virus in Culicoides Biting Midges Using a Semi-Quantitative RT-PCR Assay and Isolation of Infectious Virus
... Background: Culicoides biting midges (Diptera: Ceratopogonidae) are the biological vectors of globally significant arboviruses of livestock including bluetongue virus (BTV), African horse sickness virus (AHSV) and the ...
8
Expression profiles of myostatin and calpastatin genes and analysis of shear force and intramuscular fat content of yak longissimus muscle
... Semi-quantitative RT-PCR was employed to as- say mRNA levels of MSTN and CAST genes in tis- sues of yaks randomly chosen out of the ten adult male yaks mentioned ...Science). PCR ...
7
Expression analysis of LIM gene family in poplar, toward an updated phylogenetic classification
... The PCR was terminated with one extra step at 72°C for 10 min and hold at ...® PCR System 9700 (Applied Biosystems). Ten microliters of each PCR product were electrophoresed on a 1% agarose gel that ...
8
Original Article Prognostic significance of SOX-1 expression in human hepatocelluar cancer
... First, semi-quantitative RT-PCR and Western blot assays were performed to detect the expression of SOX-1 mRNA and protein in 15 paired of HCC tissues and corresponding nontumor ...
8
Retinoic acid-induced 2 (RAI2) is a novel tumor suppressor, and promoter region methylation of RAI2 is a poor prognostic marker in colorectal cancer
... By analyzing The Human Protein Atlas, RAI2 was found to be highly expressed in normal human colonic tissue, and its expression was reduced in colorectal cancer sam- ples (http://www.proteinatlas.org/ENSG00000131831). ...
14
How is Europe positioned for a re emergence of Schmallenberg virus?
... Measurement of the infection and dissemination of Bluetongue virus in Culicoides biting midges using a semi-quantitative RT-PCR assay and isolation of infectious virus.. Implicating Culi[r] ...
27
RASSF10 suppresses colorectal cancer growth by activating P53 signaling and sensitizes colorectal cancer cell to docetaxel
... RASSF10, semi-quantitative RT-PCR was employed in colorectal cancer cells (LOVO, RKO, DLD1, HT29, HCT116, SW480, SW620, ...specific PCR (MSP) to examine the promoter region of RASSF10, ...
12
POPK 1/Sad 1 kinase is required for the proper translocation of maternal mRNAs and putative germ plasm at the posterior pole of the ascidian embryo
... out semi-quantitative RT-PCR at the late 8-cell stage to evaluate the quantity of Hr-POPK-1 and Hr-ZF1 mRNA, and Hr-Notch mRNA as loading ...
12
Investigation of Autophagy-Related Gene Expressions in the Rat Model of Parkinson Disease
... groups, semi-quantitative analysis of the RT-PCR gel results showed that ATG101 gene was not expressed and ATG13, ATG14L, and VPS34 were downregulated in the induction group in comparison with ...
7
Immunological assays for chemokine detection in in-vitro culture of CNS cells
... direct, semi-quantitative comparison of the expression of different chemokine mRNA levels from relatively small cell numbers or small tissue ...Although RT-PCR is a sensitive tool for assaying ...
13
Regulation of ABI5 expression by ABF3 during salt stress responses in Arabidopsis thaliana
... The ABI5 gene promoter (started from the translation start site) was analyzed by PLANTCARE (http://bioin forma tics.psb.ugent .be/webto ols/plant care/html/) or AGRIS (http://arabi dopsi s.med.ohio-state .edu). Four ...
14
Characterization of mouse embryonic stem cell differentiation into the pancreatic lineage in vitro by transcriptional profiling, quantitative RT PCR and immunocytochemistry
... tive RT-PCR analysis to determine transcriptional profiles of undifferentiated wildtype (wt) and Pax4 expressing (Pax4+) ES cells and differentiated cells of committed progenitor and advanced ...
15
Airborne Rhinovirus Detection and Effect of Ultraviolet Irradiation on Detection by a Semi-Nested RT-PCR Assay
... inhibits PCR assays in single and double stranded RNA viruses,[28,29] while our RT-PCR method did not detect a difference between UV exposed and nonexposed rhinoviral ...and PCR methods could ...
8
HPV related methylation signature predicts survival in oropharyngeal squamous cell carcinomas
... First-strand cDNA was synthesized from 2 μg total RNA using the Super- script II First strand synthesis system (Invitrogen) according to the manu- facturer’s protocol. The cDNA was diluted 1:5 for use in qRT-PCR. ...
15
Broadly Reactive and Highly Sensitive Assay for Norwalk Like Viruses Based on Real Time Quantitative Reverse Transcription PCR
... all RT-PCR ...Conventional RT-PCR methods did not detect other RNA viruses, such as hepatitis A virus, hep- atitis E virus, poliovirus, Sapporo-like viruses, and Astro virus (data not ...
10
Reference gene identification for reliable normalisation of quantitative RT-PCR data in Setaria viridis
... standard RT-PCR approach and the construction of melt ...via RT-PCR analysis ...via RT-qPCR analysis across 52 tissue sam- ples ...by RT-PCR with all eleven sequences ...
12
Optimisation of qualitative and semi-quantitative detection of genetically modified crops by PCR
... and PCR programmes published by Greiner et ...introducing PCR methods of detection of Bt-maize the fi rst step was to optimise the pro- tocol for the detection of the maize genome and detection of the specifi ...
6
Concurrent Genotyping and Quantitation of Cytomegalovirus gB Genotypes in Solid Organ Transplant Recipients by Use of a Real Time PCR Assay
... and quantitative PCR (RT-GQ-PCR) assay to genotype cytomeg- alovirus (CMV) and quantify viral loads simultaneously in solid organ transplant (SOT) ...the RT-GQ-PCR with known ...
7
Internal control genes for quantitative RT PCR expression analysis in mouse osteoblasts, osteoclasts and macrophages
... the PCR amplifications were assessed by the examination of the melt curves to con- firm the presence of single gene-specific ...curves, PCR products were puri- fied through polyacrylamide gel ...eluted ...
9