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Sequence capture

Sequence capture PCR improves detection of mycobacterial DNA in clinical specimens

Sequence capture PCR improves detection of mycobacterial DNA in clinical specimens

... that sequence capture-PCR can be applied to a variety of different target ...DR sequence is always present in organisms of the ...this sequence have not been identified. In contrast, the ...

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Detection of Viruses in Clinical Samples by Use of Metagenomic Sequencing and Targeted Sequence Capture

Detection of Viruses in Clinical Samples by Use of Metagenomic Sequencing and Targeted Sequence Capture

... conserved sequence (5=-GTTTCCCAGTCACGATA-3=) followed by second-strand synthesis with Sequenase (Thermo Fisher Scientific, Waltham, ...conserved sequence using AccuPrime Taq DNA polymerase (Thermo Fisher ...

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Target sequence capture in the Brazil nut family (Lecythidaceae): marker selection and in silico capture from genome skimming data

Target sequence capture in the Brazil nut family (Lecythidaceae): marker selection and in silico capture from genome skimming data

... Target sequence capture, which sequences regions of interest after their hybridization to probes, on the other hand, has proven to be an effective method for sequencing hundreds of nuclear loci from tissue ...

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Albugo candida race diversity, ploidy and host associated microbes revealed using DNA sequence capture on diseased plants in the field

Albugo candida race diversity, ploidy and host associated microbes revealed using DNA sequence capture on diseased plants in the field

... We report here a sequence capture-based protocol (PenSeq) that enables cost-effective assessment of pathogen genetic diversity in field samples. We designed baits based on specific sequences from c. 50 ...

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Mapping by sequencing in complex polyploid genomes using genic sequence capture : a case study to map yellow rust resistance in hexaploid wheat

Mapping by sequencing in complex polyploid genomes using genic sequence capture : a case study to map yellow rust resistance in hexaploid wheat

... Survey Sequence (CSS) contigs and had been assigned a wheat chromosome cM position (IWGSC, ...the capture design contigs onto the IWGSC CSS contigs (E-value cutoff ...1e-5). Capture design contigs, ...

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Lineage specific rediploidization is a mechanism to explain time lags between genome duplication and evolutionary diversification

Lineage specific rediploidization is a mechanism to explain time lags between genome duplication and evolutionary diversification

... unique capture probes were used in BLASTn searches against the At- lantic salmon genome (ICSASG_v2; NCBI accession GCF_000233375) via ...our capture assemblies and confidence that the targeted genes were ...

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Heritable Epigenomic Changes to the Maize Methylome Resulting from Tissue Culture

Heritable Epigenomic Changes to the Maize Methylome Resulting from Tissue Culture

... The sequence capture probe set was originally designed based on the B73 RefGen_v2 (AGPv2) assembly of the maize genome and subsequently updated to B73 RefGen_v4 aka AGPv4 (also known as ...of capture ...

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Detection of Mycobacterium bovis in Bovine Clinical Specimens Using Real Time Fluorescence and Fluorescence Resonance Energy Transfer Probe Rapid Cycle PCR

Detection of Mycobacterium bovis in Bovine Clinical Specimens Using Real Time Fluorescence and Fluorescence Resonance Energy Transfer Probe Rapid Cycle PCR

... DNA sequence capture PCR, as previously described (16) and modified (23), was applied to homogenates of the 38 decontaminated bovine lymph nodes with the following additional ...biotinylated capture ...

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RAD Capture (Rapture): Flexible and Efficient Sequence-Based Genotyping

RAD Capture (Rapture): Flexible and Efficient Sequence-Based Genotyping

... as sequence capture and restriction enzyme-based methods enable the analysis of many more individuals per unit ...in-solution capture of chosen RAD tags to target sequencing reads to desired ...and ...

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Gene Discovery in Hereditary Cerebellar Cortical Degeneration of Scottish Terriers.

Gene Discovery in Hereditary Cerebellar Cortical Degeneration of Scottish Terriers.

... the sequence data from HTS, all exons of the HPRT1 gene were resequenced with the Sanger technique and the sequence data generated was ...to sequence all the variants identified by our targeted ...

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Targeted enrichment by solution-based hybrid capture to identify genetic sequence variants in barley

Targeted enrichment by solution-based hybrid capture to identify genetic sequence variants in barley

... targeted sequence capture systems with next-generation sequencing provides an efficient approach to explore target genetic regions at high resolution, and allows rapid discovery of thousands of genetic ...

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Improved Memory Network for Aspect Sentiment Analysis

Improved Memory Network for Aspect Sentiment Analysis

... We use CNN and BiLSTM to build the memory which records text local information and sequence information, the illustration is given in Figure 4. We model the semantic representation of a memory slice in two stages. ...

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The manifestations of formal standardisation and lobbying activities in innovation deals

The manifestations of formal standardisation and lobbying activities in innovation deals

... Abstract This dissertation researches the manifestations of direct lobbying activities in form of regulatory capture, cultural capture, and corporate capture as well as proposed regulato[r] ...

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Integrating shortest dependency path and sentence sequence into a deep learning framework for relation extraction in clinical text

Integrating shortest dependency path and sentence sequence into a deep learning framework for relation extraction in clinical text

... sentence sequence, which may reduce noise caused by many other entities; 2) SDP emphasizes more on syntactic structures, which are critical to the relation extraction task; and 3) The dependency relation type ...

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Real-Time Pen Input System for Writing Utilizing Stereo Vision

Real-Time Pen Input System for Writing Utilizing Stereo Vision

... Another system was implemented by H.Bunke et al. [7] for real-time handwriting acquisition by reconstructing the movement of the pen tip from a sequence of images. The distinction between pen-up and pen-down ...

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Optimization and Initial Experience of a Multisection Balanced Steady State Free Precession Cine Sequence for the Assessment of Fetal Behavior in Utero

Optimization and Initial Experience of a Multisection Balanced Steady State Free Precession Cine Sequence for the Assessment of Fetal Behavior in Utero

... cine sequence was selected for optimization, and various compromises were explored in all acquisition parameters to achieve an effective balance between anatomic coverage of the fetus and the temporal resolution ...

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Attentional capture in visual search: capture and post capture dynamics revealed by EEG

Attentional capture in visual search: capture and post capture dynamics revealed by EEG

... Sawaki and Luck 2013 reported such a sequence in a paradigm in which two displays were presented in succession: a task-irrelevant ‘cue’ display presented for 100 ms, which consisted of f[r] ...

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Rapid Design of a Bait Capture Platform for Culture- and Amplification-Free Next-Generation Sequencing of SARS-CoV-2

Rapid Design of a Bait Capture Platform for Culture- and Amplification-Free Next-Generation Sequencing of SARS-CoV-2

... SARS-CoV-2 difficult in resource-constrained countries without the facilities to culture the virus. Bait capture of SARS-CoV-2 followed by NGS using small Nanopore sequencers provides a simpler alternative (24). ...

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Trace contrast models for capture recapture without capture histories

Trace contrast models for capture recapture without capture histories

... about capture–recapture data, in preparation for a future in which animal identity might be observed only indirectly through a series of informative ...if capture–recapture is con- ducted using a ...

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Prey capture in the praying mantis Tenodera aridifolia sinensis: coordination of the capture sequence and strike movements

Prey capture in the praying mantis Tenodera aridifolia sinensis: coordination of the capture sequence and strike movements

... The contribution of the lunge to the position of the prothorax when contact with the prey occurs was evaluated by comparing d, pL with dc, HeBody position and 'absolute' prey location To[r] ...

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