Sequencing and Mapping
Nanopores and Nanochannels: From Gene Sequencing to Genome Mapping
... Hand-held sequencing devices allow performing DNA analysis at remote locations and independent of a centralized ...DNA sequencing and mapping have hence the potential to greatly facilitate ...
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User guide for mapping by sequencing in Arabidopsis
... of mapping-by- ...scheme. Mapping-by-sequencing with backcross popula- tions requires higher coverage for optimal mapping results as compared to outcross ...for mapping- ...
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Fast and sensitive mapping of nanopore sequencing reads with GraphMap
... in mapping and ...of mapping with GraphMap can be a key advantage in applications where MinION sequencing reads are used in real-time to identify pathogens 23 , particularly in combination with rapid ...
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Evaluation of next-generation sequencing software in mapping and assembly
... reads mapping and assembly software have to keep pace with these novel ...split mapping signatures and de novo sequencing, NGS sequencing machines all try to produce longer ...
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Association mapping from sequencing reads using k-mers
... Figure 1. Workflow for association mapping using k-mers. The Hawk pipeline starts with sequencing reads from two sets of samples. The first step is to count k-mers in reads from each sample. Then k-mers ...
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Association mapping from sequencing reads using k-mers
... throughput sequencing data. As sequencing gets cheaper association mapping using next generation sequencing is becoming feasi- ...Moreover, sequencing errors make genotype calling ...
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FANSe: an accurate algorithm for quantitative mapping of large scale sequencing reads
... high-throughput sequencing applications is the accurate and sensitive alignment of the sequencing reads to reference genomes or ...the sequencing platform or by misreading of modified nucleotides is ...
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Allelic mapping bias in RNA sequencing is not a major confounder in eQTL studies
... simulating sequencing er- rors, and errors in the indel calling of 1000 Genomes Phase 1 data may lead to some underestimation of the ...by mapping bias suggests that they are unlikely to be ...eliminate ...
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Comparative mapping and targeted capture sequencing of the gametocidal loci in Aegilops sharonensis
... deletion mapping experiments in case the above candidate genes were found to be ...geted sequencing has provided us with a valuable source of sequence information and possible high-density SNPs to exhaust ...
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Comparative mapping and targeted capture sequencing of the gametocidal loci in Aegilops sharonensis
... element GcB. However, none of the samples exhibited coverage over the full targeted region. Possible causes for this include errors in assembling the reference genome sequence, poor read mapping (for repeated ...
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Novel computational techniques for mapping and classifying Next-Generation Sequencing data
... DNA SEQUENCING This article has been accepted for inclusion in a future issue of this ...Read Mapping Problem 1) Data: Each next-generation sequencing platform implements a different technology, but ...
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Gene Association Mapping in the Era of Next-Generation Sequencing and Systems Biology
... MAF, sequencing based association study could generate tons of rare or low frequency variants and traditional statistical methods often fail in association mapping due to poor statistical ...
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Whole-Genome Restriction Mapping by “Subhaploid”-Based RAD Sequencing: An Efficient and Flexible Approach for Physical Mapping and Genome Scaffolding
... et al. 2012). Therefore, for most heterozygous genomes, the majority of 2bRAD tags in the genome should contain 0–1 polymorphic loci which would not affect the tag identity and mapping accuracy. In fact, RadMap ...
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Next-Generation Sequencing-Based Approaches for Mutation Mapping and Identification in Caenorhabditis elegans
... a mapping interval had been defined, a consider- able amount of work remained until the phenotype-causing mutation could be ...a mapping region were, in principle, ...Sanger sequencing of the locus ...
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Old can be new again: HAPPY whole genome sequencing, mapping and assembly
... genome mapping and sequencing methods have ad- vanced significantly to provide a foundation for scientists to understand genome structures and functions in many ...genome mapping relies on genome se- ...
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Investigating bisulfite short-read mapping failure with hairpin bisulfite sequencing data
... whether mapping efficiency can be improved without sequence recovery but with mapping information from additional sequence read, since one sequence from the hairpin sequences may uniquely map while the ...
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Transposon insertion profiling by sequencing (TIPseq) for mapping LINE-1 insertions in the human genome
... complicate sequencing read ...for mapping these elements and highlight LINE-1 continued activity in humans ...genome sequencing reads [10, 11, 18, 19, 40, ...
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An approach to comparing tiling array and high throughput sequencing technologies for genomic transcript mapping
... Calculating mean intensity for MPSS tags from tiling microarray data We sought to investigate the reason behind the low to moderate overlap for MPSS tags. This objective also addresses a related question, namely, ...
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Identification of embryonic lethal genes in humans by autozygosity mapping and exome sequencing in consanguineous families
... Results: We have studied 24 eligible families using autozygosity mapping and whole-exome sequencing. In addition to revealing mutations in genes previously linked to embryonic lethality in severe cases, our ...
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Linkage mapping in prairie cordgrass (Spartina pectinata Link) using genotyping-by-sequencing markers
... 54 calling. This method eliminates the need for the UNEAK pipeline. The UNEAK pipeline eliminates many SNPs. Using the common parent in a nested association mapping population would make a wise choice for the ...
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