Sequencing of SOLiD Total-RNA-seq libraries
Strand-specific libraries for high throughput RNA sequencing (RNA-Seq) prepared without poly(A) selection
... starting RNA [16,17]. The high sequencing depth of the Illumina HiSeq 2000 platform (Illumina, San Diego, CA, USA) can easily generate >170 million reads per lane, allowing multiple barcoded samples to ...
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Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies.
... cDNA libraries compared to unnormalised libraries in RNA-Seq ...normalised libraries were also more efficient for finding microsatellite ...and sequencing e ff orts are both likely ...
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IVT seq reveals extreme bias in RNA sequencing
... this RNA was mixed with complex mouse total RNA at various concentrations, and sequenced using the two most common RNA-seq protocols, polyA-seq or total ...
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A detailed protocol for subcellular RNA sequencing (subRNA-seq)
... is RNA sequencing (RNA-seq). RNA-seq relies on RNA isolation from whole-cell lysates and thus mainly captures fully processed, stable, and more abundant cytoplasmic RNAs ...
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Determining sequencing depth in a single-cell RNA-seq experiment
... different sequencing depths while the plug- in estimator is ...different sequencing depths, where we can see clear inflation for the plug-in ...the total number of genes for the two panels may ...
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An automated method for efficient, accurate and reproducible construction of RNA seq libraries
... next-generation RNA-seq libraries, especially for trancriptome-based annotation of larger ...Generation Sequencing Cores or Genomics Laboratories will prove an indispensable tool for ...
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Sensitive and powerful single-cell RNA sequencing using mcSCRB-seq
... 32 RNA-seq libraries from 10 pg of total RNA (UHRR) using eight replicates for each of the following four SCRB-seq protocol variants (Supplementary Tables 1 , 2 ): the original ...
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Modeling enzyme processivity reveals that RNA Seq libraries are biased in characteristic and correctable ways
... of sequencing reads for different types of ...classical RNA-seq FPKM measure, which is now generally un- derstood to be an oversimplification but still widely ...mon RNA-seq analysis ...
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Modeling Enzyme Processivity Reveals that RNA-Seq Libraries Are Biased in Characteristic and Correctable Ways
... of sequencing reads for different types of ...classical RNA-seq FPKM measure, which is now generally un- derstood to be an oversimplification but still widely ...mon RNA-seq analysis ...
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Quantifying the tradeoff between sequencing depth and cell number in single-cell RNA-seq
... For sequencing, the cDNA library is sampled and the molecules in that sample are distributed over an imaging array which is used to read out the DNA sequence of amplified products of each ...DNA sequencing ...
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Evaluation of Unbiased Next Generation Sequencing of RNA (RNA seq) as a Diagnostic Method in Influenza Virus Positive Respiratory Samples
... the total number of assembled sequences (on average, ...amplicon sequencing of bac- terial rRNAs, UMERS-derived contigs originating from highly conserved sequences can hold sufficient discriminatory power ...
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Quality assessment and control of tissue specific RNA-seq libraries of Drosophila transgenic RNAi models
... However, RNA-seq is not a bias free ...of RNA and library preparation can introduce multiple artifacts, compounded by problems from later stages in the ...Nevertheless, RNA-seq is ...
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BRB seq: ultra affordable high throughput transcriptomics enabled by bulk RNA barcoding and sequencing
... use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis ...cDNA libraries for dozens of samples, requiring just 2 hours of hands-on ...lower ...
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SCOPE Seq: a scalable technology for linking live cell imaging and single cell RNA sequencing
... enabling libraries of biomolecules to be captured on beads in solution and decoded by ...microarray, sequencing, and flow-based expression profiling ...Expression sequencing (SCOPE-Seq) to ...
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Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
... the RNA-seq data. This consideration is not unique to Gel-seq, but is a common aspect of low-input RNA-seq library ...during sequencing library preparation is often necessary, ...
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Natural Killer (NK) cell receptor expression in cattle: development of a pipeline for accurate quantification of RNA Sequencing (RNA-Seq) data
... of total transcription of the genes of each complex is highly variable between ...higher total transcription does not equate with higher transcription of each individual ...the total transcription in ...
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VIPER: Visualization Pipeline for RNA-seq, a Snakemake workflow for efficient and complete RNA-seq analysis
... next-generation sequencing methods yield fastq files that contain the sequencing reads captured from the ...In RNA-seq, the reads after alignment are quantified on a per gene or per transcript ...
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Elimination of PCR duplicates in RNA-seq and small RNA-seq using unique molecular identifiers
... However, sequencing libraries made from a small number of cells or from a small amount of tissue or RNA, have become increasingly common [ 46 ], and they are more severely affected by PCR ...
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RNA Preparation, QC and Shipping for RNA-Seq Applications
... A Sample Submission Form (provided by e-mail at time of order), a copy of the quote or purchase order, as well as any relevant QC and safety data must accompany each package to be shipped, for each service project. For ...
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Bioinformatics for RNA‐Seq Data Analysis
... degraded RNA. After quality and quantity of the RNA samples have been addressed, one must then choose whether to profile the total RNA space or the mRNA space ...most RNA‐seq ...
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