Simian virus 40 large T antigen
Differential phosphorylation of cytoplasmic and nuclear variants of simian virus 40 large T antigen encoded by simian virus 40-adenovirus 7 hybrid viruses.
... 636 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The phosphorylation patterns of cytoplasmic and nuclear forms of simian virus 40 large T antigen encoded by simian v[r] ...
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Replication and transformation functions of in vitro-generated simian virus 40 large T antigen mutants.
... Mutant of simian virus 40 large T-antigen that is defective for viral DNA synthesis, but competent for transformation of cultured rat cells.. Simian virus 40 deoxyribonucleic acid synthe[r] ...
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ATP enhances the binding of simian virus 40 large T antigen to the origin of replication.
... 12 0022-538X/87/123649-06$02.00/0 Copyright © 1987, American Society for Microbiology ATP Enhances the Binding of Simian Virus 40 Large T Antigen the Origin of Replication to SWATI PALIT[r] ...
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Identification of a novel antiapoptotic functional domain in simian virus 40 large T antigen.
... sus N-termTags suggested the existence of a novel antiapop- totic activity in the full-length protein. In this study, we identified a functional region of T antigen which protects against apoptosis ...
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Immunological Cross-Reactivity Between Simian Virus 40 Large T Antigen and D2 Hybrid T Antigen
... Proteins were immunoprecipitated from these extracts by using the antiserum produced in rabbits against purified D2 hybrid T antigen rabbit anti-D2, and these proteins were compared with[r] ...
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A novel translational regulation function for the simian virus 40 large-T antigen gene.
... the large-T-specific mRNAs produced in CV-1p cells transfected with p6-1, which contains a WT large-T gene, or pR137, which contains a mutant large-T gene with a mutation in the ...
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Oligomerization and origin DNA-binding activity of simian virus 40 large T antigen.
... In contrast, studies carried out with lytic T-antigen subclasses separated by zone velocity sedimentation or phosphocellulose chromatography showed that newly synthesized 5 to 7S T antig[r] ...
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Detection and characterization of multiple forms of simian virus 40 large T antigen.
... Simian virus 40 SV40 DNA codes for two early proteins, the large and small tumor T antigens 22, 30, 33, which can be immunoprecipitated from extracts of cells productively infected or tr[r] ...
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Crystal Structure of the Simian Virus 40 Large T-Antigen Origin-Binding Domain
... the T-ag obd has also been ...of T-ag to form double hexamers ...arranged T-ag obd’s. (A) Surface representation of the T-ag obd ...arranged T-ag obd’s is presented below each view to ...
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Two separable functional domains of simian virus 40 large T antigen: carboxyl-terminal region of simian virus 40 large T antigen is required for efficient capsid protein synthesis.
... COLE',2* Department Genetics, Yale University School of Medicine, New Haven, Connecticut 065101; and Department of Biochemistry, Dartmouth Medical School, Hanover, New Hampshire 037562* [r] ...
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Quantitative Analysis of the Binding of Simian Virus 40 Large T Antigen to DNA
... another T-ag molecule, albeit at high concentrations, as would be the case for two T-ag molecules bound next to each other on ...of T-ag regu- lates oligomerization of the OBD, in agreement with the ...
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Use of simian virus 40 large T-beta-galactosidase fusion proteins in an immunochemical analysis of simian virus 40 large T antigen.
... Inhibition of binding of '25I-labeled pure large T antigen to the immobilized antibody in the presence of the fusion protein cell extract containing ,-galactosidase alone was used as a c[r] ...
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Simian virus 40 large T antigen associates with cyclin A and p33cdk2.
... The most likely candidates for such a linker are the recessive oncoproteins pRb and p107, both of which can bind cyclin A and p33cdk2 independently of viral oncoproteins 14, 23, but it i[r] ...
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The zinc finger region of simian virus 40 large T antigen.
... Such a change in conformation could affect multiple independent functions, including origin binding, transformation, and the function of the active site element of the zinc finger in rep[r] ...
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Measurements of the molecular size of the simian virus 40 large T antigen.
... This would be 218 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest A measure of the molecular weight of the large simian virus 40 T antigen was sought by SDS-polyacrylam[r] ...
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Identification of a region of simian virus 40 large T antigen required for cell transformation.
... However, mutants which lack origin-binding activity retain the ability to transform both primary and established cells phenotypically, indicating that It has been known for some time tha[r] ...
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Metabolic turnover of phosphorylation sites in simian virus 40 large T antigen.
... Thus, for all lysates, the total 32P radioactivity incorporated in 88K large T molecules 0 is shown, as well as the 32p radioactivity associated with the N-terminal P-Ser site l, the N-t[r] ...
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Simian virus 40 large T antigen: the puzzle, the pieces, and the emerging picture.
... Overexpres1289 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest INTRODUCTION: THE PUZZLE In cultured monkey cells infected by simian virus 40 SV40, the viral regulatory p[r] ...
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Mapping the transcriptional transactivation function of simian virus 40 large T antigen.
... Two mutants with lesions in the DNA-binding domain that were unable to bind to SV40 DNA were completely defective for transactivation of both promoters, while a third mutant with a lesio[r] ...
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Functional characterization of temperature-sensitive mutants of simian virus 40 large T antigen.
... Purified T antigens T ag were either not exposed - or exposed + to a heat shock for 10 min at 39°C, incubated in replication buffer containing plasmids with the SV40 origin of replicatio[r] ...
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