Single live cell imaging
A high speed multifocal multiphoton fluorescence lifetime imaging microscope for live-cell FRET imaging
... from single-photon events on 32 × 32 pixels at up to 500 kcts/sec/pixel[28] leading to a frame rate which scales linearly with detector ...a single detector (assuming photon counting rates of 1% for an ...
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Optoelectronic tweezers system for single cell manipulation and fluorescence imaging of live immune cells
... hybridoma cell line, modified to express EGFP (enhanced green fluorescent protein) following NFAT-activation [14], was cultured in RPMI 1640 medium supplemented with fetal calf serum (10%), penicillin and ...or ...
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Quantitative single cell live imaging links HES5 dynamics with cell state and fate in murine neurogenesis
... short-term single cell variability but through a combination of cell sub-states co-existing in the tissue (which can be resolved spatially and dynamically) and transitions between these sub- ...in ...
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Dissecting the molecular mechanisms of drosophila border cell migration using time-lapse live cell imaging
... time-lapse imaging is analysed correctly and is consistent across multiple egg ...border cell migration was found to be through tracking the movement of the ...in single frames and lack of polar ...
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Quantitative live cell imaging of glucocorticoid receptor dynamics in the nucleus
... a single electron, a collective oscillation of the free electrons on the surface of the particle is generated in a process termed plasmon ...background-free imaging of gold nanorods in living cells ...
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A live cell imaging system for visualizing the transport of Marburg virus nucleocapsid like structures
... The main nucleocapsid protein of MARV is NP, which is responsible for the encapsidation of single-stranded viral genomic RNA [3, 4]. In addition to NP, MARV nu- cleocapsids also contain the minor matrix protein ...
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Practical three color live cell imaging by widefield microscopy
... color imaging of proteins in living cells or very long term studies of single protein expression by 4 dimensional (x,y,z,time) analysis, a technique requiring a photostable ...
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Accelerating Live Single-Cell Signalling Studies.
... Studying cell cycle regulation, Spencer et ...a cell will proliferate or quiesce ...the live-cell reporter revealing the formation of two stable subpopulations shortly after ...studying ...
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Live imaging of individual cell divisions in mouse neuroepithelium shows asymmetry in cilium formation and Sonic hedgehog response
... monitor cell divisions in the developing embryo, we optimized culture conditions for whole embryos and confirmed that morphological development and neural patterning proceeded normally over a 24- hour period in ...
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Live cell imaging reveals marked variability in myoblast proliferation and fate
... itional cell division, our interpretation is that the main action of IGF-I is to maintain myoblast survival so that otherwise vulnerable cells are able to complete a single final round of ...
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SCOPE Seq: a scalable technology for linking live cell imaging and single cell RNA sequencing
... from live cell imaging data ...monochrome live cell im- aging data (Additional file 1: Supplementary Methods, ...our imaging data and attempted to identify multiplets by manually ...
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Live-cell imaging of macrophage phagocytosis of asbestos fibers under fluorescence microscopy
... as live cell imaging for the study of asbestos in this ...is live-cell imaging incorporating fluorescence microscopy ...of single chrysotile fibrils ...and ...
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Hydrothermal synthesis of nitrogen-doped carbon dots with real-time live-cell imaging and blood–brain barrier penetration capabilities
... The size and morphology of the hydrothermally synthesized N-CDs were examined using TEM. The TEM images (Figure 2A and B) reveal that the N-CDs are mono- dispersed and form semispherical shape with an approximate ...
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Cell lineage visualisation
... of cell deaths, an event type of great interest, is shown with the height of red ...shown. Cell deaths are in ...of cell divisions for a lineage where every cell divides into two daughter ...
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TriPer, an optical probe tuned to the endoplasmic reticulum tracks changes in luminal H2O2
... glutathione into the isolated rat liver microsomal fraction has been observed [7], and in a functional counterpart to these experiments, excessive reduced glutathione on the cytosolic side of the plant cell ER ...
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Live Cell Imaging of Phosphoinositide Dynamics and Membrane Architecture during Legionella Infection
... ⌬ sidC-sdcA strain, PtdIns(4)P-positive LCVs were formed, as oc- curred with wild-type bacteria. However, the recruitment of calnexin-GFP to PtdIns(4)P-positive LCVs was severely impaired, yielding obvious ...
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Axonal BACE1 dynamics and targeting in hippocampal neurons: a role for Rab11 GTPase
... by live-cell imaging in cultured hippocampal ...dual-color imaging, we demonstrate BACE1-YFP localization and dynamic transport in Rab11-positive recyc- ling ...
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Optimisation of the synthesis and modification of CdTe quantum dots for enhanced live cell imaging
... the cell membrane are internalized and now exhibit a diffuse cytoplasmic and an enhanced nuclear membrane ...the cell region making them ideal tools for biomedical ...on cell viablility (data not ...
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Clarification of mural cell coverage of vascular endothelial cells by live imaging of zebrafish
... lapse imaging revealed that MCs preferentially extended their processes along inter-EC junctions during their migration along the CtA ...their cell bodies to the scaffold structures formed within the ...
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Increased PKMζ activity impedes lateral movement of GluA2-containing AMPA receptors
... For whole-cell patch-clamp recordings, rat cultured hippocampal neurons at DIV 13–14 were used for patch clamp recording. Neurons were voltage clamped at −70 mV for mEPSC recording, using a Multiclamp 700B ...
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