Single molecule force
Efficient unfolding pattern recognition in single molecule force spectroscopy data
... Single-molecule force spectroscopy is a promising method for measuring the unfolding forces of single molecules and cells. SMFS can analyze membrane pro- teins in their natural membrane ...
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A peptide‐display protein scaffold to facilitate single molecule force studies of aggregation‐prone peptides
... used single molecule force spec- troscopy (SMFS) to probe the first dimerization events of the central aggregation-prone region of aSyn (residues 71–82) that may initiate ...
13
Single-molecule force spectroscopy on histone h4 tail cross-linked chromatin reveals fiber folding
... employed single-molecule force spec- troscopy to measure the unfolding of individual chromatin fibers that are reversibly cross-linked through the histone H4 ...obtained force-extension curves ...
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A peptide‐display protein scaffold to facilitate single molecule force studies of aggregation‐prone peptides
... used single molecule force spectroscopy (SMFS) to probe the first dimerisation events of the central aggregation-prone region of Syn (residues 71-82) that may initiate ...
50
Ott, Wolfgang Bernhard (2017): Single molecule force spectroscopy with biological tools. Dissertation, LMU München: Fakultät für Physik
... interface, force propagated along paths with strong normal components to the pulling ...the force across the binding interface at high ...applied force toward an unfavorable angle of attack at the ...
305
Klamecka, Kamila (2017): Single-molecule force spectroscopy of biological complexes. Dissertation, LMU München: Fakultät für Biologie
... to single digits) number of molecules picked from a huge batch - be it a few molecules displayed on the cantilever or a few tethers analyzed within an MT ...
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Life in extreme environments: Single molecule force spectroscopy as a tool to explore proteins from extremophilic organisms
... the force required to unfold the protein decreases with increasing temperature ...unfolding force with temperature in combination with Monte Carlo ...
14
Application of Fuzzy Logic Algorithm to Single Molecule Force Spectroscopy of the Streptavidin Biotin System
... of single-molecule interaction forces of the streptavi- din-biotin ligand-receptor ...extract single molecular events from 22,500 force-separation curves obtained from AFM measurements, we em- ...
10
Single-molecule force spectroscopy studies of fibrin A-a polymerization interactions via the atomic force microscope
... 1.1.1 Fibrin(ogen) structure. Fibrinogen, a 340 kDa protein, is a symmetric, hexameric protein, consisting of three pairs of identical chains: the A α , B β , and γ ( Figure 1.1A). 3, 5, 10-13 The N-termini of all six ...
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Extraction of accurate biomolecular parameters from single-molecule force spectroscopy experiments
... a single protein is extended between the tip and AFM substrate, the protein is usually immobilized directly to the mica, 24 glass, 25 27 or gold 28 30 substrate and attached to the tip via nonspecific ...to ...
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Single-Molecule Unbinding Forces between the Polysaccharide Hyaluronan and Its Binding Proteins
... based single-molecule force spectros- copy (SMFS) to quantify the response of individual molecular interactions to tensile ...This force probe technique is now well established for the ...
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Rapid and Robust Polyprotein Production Facilitates Single-Molecule Mechanical Characterization of β-Barrel Assembly Machinery Polypeptide Transport Associated Domains.
... . Single molecule force unbinding studies 43, 44 are ideally suited to address this question, but a pre-requisite to such a study is the full charac- terisation of the mechanical properties of the ...
11
Jobst, Markus A. (2018): Multiplexed single molecule observation and manipulation of engineered biomolecules. Dissertation, LMU München: Fakultät für Physik
... of single amino acids on specific functions or stability. In force spectroscopy assays, addition and exchange of binding tags and mutations to the domains of interest are frequently performed to adapt to ...
245
Toehold-enchanced lna probes for selective pull down and single-molecule analysis of native chromatin
... impedes single molecule force spectroscopy experiments, which have been instrumental to probe chromatin ...subsequent single molecule analysis by magnetic ...of force. We employ ...
9
Nanomechanical and thermophoretic analyses of the nucleotide-dependent interactions between the AAA+ subunits of magnesium chelatase
... retained. Single molecule force spectroscopy (SMFS) was used to determine the factors that stabilize formation of the ChlID − MgADP complex at the single molecule level; ChlD was ...
7
Baumann, Fabian (2016): Atomic force microscopy for high-specificity manipulations of proteins and high-throughput analysis in nanoapertures. Dissertation, LMU München: Fakultät für Physik
... the single electron in the hydrogen shell is remarkably pulled towards this higher negative charge and thus exposes the protonic ...same molecule) by electrostatic interaction of the partially exposed ...
249
Nano-mechanical mapping of the interactions between surface-bound RC-LH1-PufX core complexes and cytochrome c (2) attached to an AFM probe
... thousand force–distance cycles, up to several hours) to a functionalised AFM probe or surface, while sustaining significant force stresses (up to 500 pN) (Schmidt et ...
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Geometric Phases in Single Molecule Magnets
... as Single Molecule Magnets (SMMs), see ...each molecule is a magnet in of itself as opposed to conventional bulk magnets that require long range magnetic ordering of their constituent magnetic dipole ...
71
Deep Learning for Single-Molecule Science
... Ideally, one would like to look for common features in the data without making prior assumptions and be able to quantify those in a coherent manner. Only recently, a Machine Learning (ML) based technique has been ...
24
Advances in single molecule nucleic acid sequencing
... directly reflected in the abundance of any particular sequence tag within the population of molecules and therefore offers a digital profiling of gene expression. Poisson statistics should apply to counting models using ...
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