Single molecule force spectroscopy
Efficient unfolding pattern recognition in single molecule force spectroscopy data
... Single-molecule force spectroscopy is a promising method for measuring the unfolding forces of single molecules and cells. SMFS can analyze membrane pro- teins in their natural membrane ...
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Single-molecule force spectroscopy on histone h4 tail cross-linked chromatin reveals fiber folding
... more gradual unstacking transition for 167-bp NRL MT fibers under reducing conditions (Fig. 4A). Likewise, sedimentation velocity analysis of 167- and 177-bp NRL MT fibers under reducing conditions indicated a reduced ...
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Klamecka, Kamila (2017): Single-molecule force spectroscopy of biological complexes. Dissertation, LMU München: Fakultät für Biologie
... to single digits) number of molecules picked from a huge batch - be it a few molecules displayed on the cantilever or a few tethers analyzed within an MT ...
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Ott, Wolfgang Bernhard (2017): Single molecule force spectroscopy with biological tools. Dissertation, LMU München: Fakultät für Physik
... interface, force propagated along paths with strong normal components to the pulling ...the force across the binding interface at high ...applied force toward an unfavorable angle of attack at the ...
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Verdorfer, Tobias (2018): Multiplexed single-molecule force spectroscopy and activity studies on cellulosomes. Dissertation, LMU München: Fakultät für Physik
... a single cantilever in two separate experiments by utilizing a fast and parallelized sample preparation method, while still achieving su ffi cient statistics (N = 1420 in 24 h and N = 7869 in 72 ...Particularly ...
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Opfer, Jan (2012): Single-molecule force spectroscopy studies of integrin-mediated cell signaling. Dissertation, LMU München: Fakultät für Physik
... T lymphocytes are important components of the adaptive, cell-mediated immune response. They circulate in the blood stream until they detect a site of inflammation. There they strongly adhere against the shear ...
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Life in extreme environments: Single molecule force spectroscopy as a tool to explore proteins from extremophilic organisms
... the force required to unfold the protein decreases with increasing temperature ...unfolding force with temperature in combination with Monte Carlo ...
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Application of Fuzzy Logic Algorithm to Single Molecule Force Spectroscopy of the Streptavidin Biotin System
... of single-molecule interaction forces of the streptavi- din-biotin ligand-receptor ...extract single molecular events from 22,500 force-separation curves obtained from AFM measurements, we em- ...
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Single-molecule force spectroscopy studies of fibrin A-a polymerization interactions via the atomic force microscope
... 1.1.1 Fibrin(ogen) structure. Fibrinogen, a 340 kDa protein, is a symmetric, hexameric protein, consisting of three pairs of identical chains: the A α , B β , and γ ( Figure 1.1A). 3, 5, 10-13 The N-termini of all six ...
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Extraction of accurate biomolecular parameters from single-molecule force spectroscopy experiments
... a single protein is extended between the tip and AFM substrate, the protein is usually immobilized directly to the mica, 24 glass, 25 27 or gold 28 30 substrate and attached to the tip via nonspecific ...to ...
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A single molecule assay to probe monovalent and multivalent bonds between hyaluronan and its key leukocyte receptor CD44 under force
... few single molecule methods are currently available to study how monovalent and multivalent GAG·protein bonds respond to directed mechanical ...with single molecule force ...
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A peptide‐display protein scaffold to facilitate single molecule force studies of aggregation‐prone peptides
... used single molecule force spectroscopy (SMFS) to probe the first dimerisation events of the central aggregation-prone region of Syn (residues 71-82) that may initiate ...
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Observation of Single-Molecule Rotational Diffusion at Microsecond Timescale by Polarized Fluorescence Correlation Spectroscopy
... tip-sample force field at eleven evenly spaced points along the sample nanotube cross ...section. Force field data sets were combined with the known me- chanical properties of the AFM cantilever and ...
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Force detected, single molecule spectroscopy and imaging using nanoscale mechanical resonators
... which single molecules in solution are detected by the mechanical ...a molecule bound to the monolayer and the resulting dipole-dipole interaction creates a shift in the resonance frequency of the ...
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Combined single-molecule force and fluorescence measurements for biology
... some single-molecule experiments ...resolution single-molecule experiments, sug- gesting that quantum dots may become invaluable in the field of single-molecule probes ...a ...
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A peptide‐display protein scaffold to facilitate single molecule force studies of aggregation‐prone peptides
... Single molecule approaches using fluores- cence, 5,6 or force spectroscopy using either the AFM 7–17 or optical tweezers 18 are potentially power- ful methods to interrogate aggregating ...
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Comparative Studies on the Interaction Between the Medicine Small Molecule with Pepsin by Fluorescence Quenching Spectroscopy and Improved Spectroscopy
... fluorescence spectroscopy with focus on the fluorescence change of protein, as well as the improved spectroscopy with focus on the fluorescence changes of the resonance light scattering of small ...
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Rigid Molecule Docking: FPGA Reconfiguration for Alternative Force Laws
... ing molecule images and correlation ...larger molecule mod- els to be split into parts, staged in on-board memory near the FPGA, and reassembled into larger ...
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Inferring diffusion in single live cells at the single-molecule level
... generate single particle trajectories in the TIRF evanescent field in the specimen focal plane, corresponding to localization of either the Helix1021 or TatA in the cytoplasmic ...the single ...
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Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single cell force spectroscopy
... Tip-less silicon nitride cantilevers (Arrow TL-1, NanoWorld, Switzerland), with force constant 0.03N/m, were used for conducting cell-to-cell adhesion and single cell indentation experiments. The actual ...
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