Single Particle Cryo-EM
Single-particle cryo-EM using alignment by classification (ABC): the structure of Lumbricus terrestris haemoglobin
... such single-particle cryo-EM processing is ‘reference bias’, which results from correlation alignment of noisy images with respect to specific references (Boekema et ...
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LAT1 (SLC7A5) and CD98hc (SLC3A2) complex dynamics revealed by single-particle cryo-EM
... work, cryo-EM structures of LAT1– CD98hc in the inward-facing conformation and in either the substrate-bound or apo states were reported to ...resolution cryo-EM structure, and multibody 3D ...
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Using a SMALP platform to determine a sub nm single particle cryo EM membrane protein structure
... using cryo-EM including Piezo, Ryanodine receptor, TRPV1 and the Glutamate ...the EM field hold the promise of even greater progress in terms of greater resolution, which for membrane proteins is ...
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Cryo EM of amyloid fibrils and cellular aggregates
... in single-particle cryo-EM have enabled the structure determination of amyloid fibrils from post-mortem human brain ...that cryo-EM allows near-atomic char- acterization of ...
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Volta phase plate cryo-EM of the small protein complex Prx3
... from cryo-EM maps 2 ...high-resolution cryo-EM images are ...include particle size, rigidity, sample purity, orientation coverage and ice thickness 6 ...for ...
5
The 3.3 Å structure of a plant geminivirus using cryo-EM
... using single particle cryo-EM ...a cryo-EM dataset collected (see ...1 particle from which a penton of CP subunits is ...
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Beta-Propiolactone Inactivation of Coxsackievirus A16 Induces Structural Alteration and Surface Modification of Viral Capsids
... a single-stranded positive-sense RNA genome of ⬃ ...“135S-like particle,” which has been resolved to atomic detail by X-ray crystallography ...virus-like particle (VLP) ...by cryo-electron ...
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A microtubule RELION based pipeline for cryo EM image processing
... sequential single-iteration 3D refine- ments to the ...between particle and reference that are closer to aligning the seam will produce higher cross-correlations (Supplementary ...a single Rot angle ...
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Blob-ology and biology of cryo-EM: an interview with Helen Saibil
... and cryo-EM fit into the current drive for reproducibility in science — data sharing, for example; and is the field working in the same direction in this respect? In single particle analysis ...
7
Approaches to altering particle distributions in cryo-electron microscopy sample preparation
... of EM in microscope hardware, automation of data collection and methods of data processing in the last five years, for the vast majority of cryo-EM users plunge freezing has remained the only method ...
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Blob ology and biology of cryo EM: an interview with Helen Saibil
... and cryo-EM fit into the current drive for reproducibility in science — data sharing, for example; and is the field working in the same direction in this respect? In single particle analysis ...
8
Structure, assembly and dynamics of macromolecular complexes by single particle cryo-electron microscopy
... also cryo-immobilizes their different conformational ...a single class; however it contains essential information about the dynamic properties of the ...either single-particle tomography or ...
8
Postentry Neutralization of Adenovirus Type 5 by an Antihexon Antibody
... the cryo-EM reconstruction indi- cates that there are at least three intact Abs bound per asym- metric unit of the viral ...the cryo-EM reconstruction is consistent with our neutralization ...
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X-ray and cryo-EM structures of inhibitor-bound cytochrome bc₁ complexes for structure-based drug discovery
... in single- particle cryo-electron microscopy (cryo-EM), an increasing number of high-resolution structures have been determined, therefore the technique has the potential to play a role ...
11
Structural Study of Piezo Channel, a Unique Family of Eukaryotic Mechanosensitive Channel
... The Transient Receptor Potential (TRP) channel family consists of ion channels that respond to a wide variety of stimulants, including chemical, temperature, and mechanical stimuli. Aside from the yeast TRPY, members of ...
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Farnung, Lucas (2017): Nucleosome-Chd1 structure and implications for chromatin remodelling. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... core particle presented a breakthrough in the understanding of chromatin at an atomic ...high-resolution single particle cryo-electron microscopy (cryo-EM), it is now possible to ...
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Poliovirus RNA Is Released from the Capsid near a Twofold Symmetry Axis
... single-particle cryo-electron microscopy. (A) Cryo-EM reconstruction of 80S poliovirus showing the footprint of the density that corresponds to RNA: red indicates a higher contour level ...
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A national facility for biological cryo electron microscopy
... for single particles, but the sample thickness must be well under 1 mm for TEM to avoid limitations caused by multiple scattering of electrons passing through the specimen and other electron optical ...a ...
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High-resolution cryo-EM proteasome structures in drug development.
... crystallography has been pivotal in providing structural information for the development of proteasome inhibitors as therapeutic agents (Groll & Huber, 2004; Borissenko & Groll, 2007). However, the field of ...
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Neyer, Simon (2017): RNA polymerase I complex structures elucidate mechanisms of transcription initiation and elongation. Dissertation, LMU München: Fakultät für Chemie und Pharmazie
... grids (Quantifoil) were glow-discharged for 20 s before applying 5 µ L sample and in- cubated for 1 min. Grids were washed by oating on a 0.5 mL drop of distilled water for 1 min, stained for 1 min on a 50 µ L drop of 2 ...
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