Small RNA
Isolation and detection of small RNA molecules
... of small RNA ...2004). Small interfering RNAs (siRNAs) have two major size classes ~21 and ~24nt and are also generated by Dicer enzymes from long double-stranded RNAs (Baulcombe 2004, Matzke and ...
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The Heterocyst Specific NsiR1 Small RNA Is an Early Marker of Cell Differentiation in Cyanobacterial Filaments
... ABSTRACT Differentiation of single cells along filaments of cyanobacteria constitutes one of the simplest developmental patterns in nature. In response to nitrogen deficiency, certain cells located in a semiregular ...
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Ancient and novel small RNA pathways compensate for the loss of piRNAs in multiple independent nematode lineages
... To confirm the loss of piRNAs, we explored the conservation of proteins involved in the RNA interference (RNAi) pathway, as defined in C. elegans (Fig. 3D). We found high conserva- tion of the proteins involved in ...
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Small RNA pathways in Schmidtea mediterranea
... the small RNA and a Piwi domain that carries out endonuclease activity (Hutvagner et ...of small RNAs associated with Piwis, revealed that different Piwi proteins interact with different sizes of ...
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Altered Fecal Small RNA Profiles in Colorectal Cancer Reflect Gut Microbiome Composition in Stool Samples
... for small RNA sequencing ...multiplex small RNA library prep set for Illumina (protocol E7330; New England BioLabs ...of RNA was used as the starting material to prepare ...multiplex ...
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A Small-RNA Enhancer of Viral Polymerase Activity
... svRNAs function to promote full-length vRNA synthesis by the RdRp. As IAV produces svRNAs from cRNA (Fig. 1C), we next sought to investigate if svRNAs could act upon this template to modulate RdRp activity. To this end ...
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Emerging role of a novel small non-coding regulatory RNA: tRNA-derived small RNA
... of small non-coding RNAs, such as miRNA and piRNA, has dramatically changed our understanding of the role RNA plays in ...novel small non-coding RNA generated from cleavage of tRNA or ...
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Functionalization of an antisense small RNA
... Genetic material is, paraphrasing the words of Alphonse de Lamartine brought to this context, limited in its nature but infinite in its desires. Four combinatorial bases offer the possibility of encoding vast information ...
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SePIA: RNA and small RNA sequence processing, integration, and analysis
... of RNA-seq protocols exist to produce total RNA, poly(A)-derived RNA and small RNA ...of RNA-seq protocol affects the design principles implemented in the computational analysis ...
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Changes in Aphid Host Plant Diet Influence the Small RNA Expression Profiles of Its Obligate Nutritional Symbiont, Buchnera
... ABSTRACT Plants are a difficult food resource to use, and herbivorous insects have evolved a variety of mechanisms that allow them to fully exploit this poor nutri- tional resource. One such mechanism is the maintenance ...
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A Central Small RNA Regulatory Circuit Controlling Bacterial Denitrification and N2O Emissions
... the RNA-seq analysis, we noted that the GntR- type regulator identified displayed conserved expression patterns identical to those seen with sRNA-29 under the various conditions that we had tested previously ...
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Genome-Wide Analysis of RNA Secondary Structure in Eukaryotes
... rRNA-depleted RNA samples were treated with a double- strand specific ribonuclease per manufacturer’s instructions (RNase V1, Applied Biosystems (Foster City, ...fragmented RNA sample was then used as the ...
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“APPLICATIONS OF RNA INTERFERENCE TECHNOLOGIES IN PHARMACEUTICAL FIELDS” by Mrinal Singha, Manju Tiwari, Ravi Thakkar, India.
... system, RNA interference (RNAi) which belongs to one type of double stranded RNA molecule of 20-25 nucleotides in length, cause sequence specific post- transcriptional gene ...messenger RNA (mRNA) ...
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RNA-Based Immunity Terminates Viral Infection in Adult Drosophila in the Absence of Viral Suppression of RNA Interference: Characterization of Viral Small Interfering RNA Populations in Wild-Type and Mutant Flies
... virus-derived small RNA pools in WT and mutant fruit ...bars)-strand small RNAs showing perfect match with the two viral genomic RNAs in WT and loqs, dcr-2, and ago2 mutant flies inoculated with FHV ...
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Regulatory role of microRNA in mesenteric lymph nodes after Salmonella Typhimurium infection
... Salmonellosis is a gastrointestinal disease caused by non‑typhoidal Salmonella serovars such as Salmonella Typhimu‑ rium. This pathology is a zoonosis, and food animals with subclinical infection constitute a vast ...
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A Unique cis Encoded Small Noncoding RNA Is Regulating Legionella pneumophila Hfq Expression in a Life Cycle Dependent Manner
... cis-encoded small RNA named Anti-hfq that is transcribed antisense of the hfq transcript and overlaps its 5= untranslated ...the small noncoding RNA (sRNA) and hfq mRNA are bound and ...
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Interference among defective interfering particles of vesicular stomatitis virus.
... Synthesis of a small RNA in cells coinfected by standard and defective interfering particles of vesicular stomatitis virus.. RNA synthesis of vesicular stomatitis virus.[r] ...
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RNA synthesis of vesicular stomatitis virus. X. Transcription and replication by defective interfering particles.
... Although the bulk of intracellular RNA synthesis specific to DI particles is known to be under replicative and not transcriptive control 23, it is not clear whether this small RNA is the[r] ...
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Reprogramming of Small Noncoding RNA Populations in Peripheral Blood Reveals Host Biomarkers for Latent and Active Mycobacterium tuberculosis Infection
... yielded small RNA biomarkers that can be used to identify difficult-to-diagnose TB cases regardless of level of lung injury (as seen on CXR images), bacterial load, or clinical ...for RNA amplifica- ...
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miR2118-triggered phased siRNAs are differentially expressed during the panicle development of wild and domesticated African rice species
... 21-nt small RNA fraction in ...21-nt small RNAs mapped to these 892 loci, but were not detected as phased siRNAs by our ...21-nt small RNA se- quences associated with these 892 phased ...
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