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Small RNA analysis by next generation sequencing

Next-generation sequencing analysis and RNA editing in human brain and glioma

Next-generation sequencing analysis and RNA editing in human brain and glioma

... WORK RNA sequencing has changed our view of transcriptomics in several ...Direct sequencing of expressed transcripts allows a global view of RNA species that are present in the cell, rather ...

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Statistical analysis of RNA-seq data from next-generation sequencing technology

Statistical analysis of RNA-seq data from next-generation sequencing technology

... We have provided a framework for building the approximated most average-powerful (AMAP) test that can be used to compare gene (Chapter 2 ) or exon (Chapter 3 ) expressions from RNA-seq data. Compared with previous ...

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GeneSifter: Next Generation Data Management and Analysis for Next Generation Sequencing

GeneSifter: Next Generation Data Management and Analysis for Next Generation Sequencing

... data analysis for a small group of samples may involve analyzing entire genomes or screening hundreds of millions of sequence reads; in a world of network computing, many labs have reverted to shipping data ...

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Next generation sequencing

Next generation sequencing

... Abstract Next-generation sequencing (also known as massively parallel sequencing) technologies are revolutionising our ability to charac- terise cancers at the genomic, transcriptomic and ...

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Next Generation Sequencing

Next Generation Sequencing

... ribosomal RNA) that is similar across species but differs by a sufficient amount at certain hypervariable locations to allow identification of at least the genus of a bacterial ...

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Applications and data analysis of next-generation sequencing

Applications and data analysis of next-generation sequencing

... data analysis of next-generation sequencing Applikationen und Datenanalyse von Next Generation Sequencing Abstract: Over the past 6 years, next-generation ...

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Algorithms for Next Generation Sequencing Data Analysis

Algorithms for Next Generation Sequencing Data Analysis

... In particular, the unspliced aligners are usually based on the seed technique or on the Burrows-Wheeler transform, which is used to index the reference sequence. The ad- vantage in using this transform is that it is ...

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Virtual Environment for Next Generation Sequencing Analysis

Virtual Environment for Next Generation Sequencing Analysis

... for RNA- Seq reads. It aligns RNA-Seq reads to mammalian-sized genomes using the ultra high-throughput short read aligner Bowtie, and then analyzes the mapping results to identify splice junctions between ...

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Virtual Environment for Next Generation Sequencing Analysis

Virtual Environment for Next Generation Sequencing Analysis

... for RNA- Seq reads. It aligns RNA-Seq reads to mammalian-sized genomes using the ultra high-throughput short read aligner Bowtie, and then analyzes the mapping results to identify splice junctions between ...

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ANGSD: Analysis of Next Generation Sequencing Data.

ANGSD: Analysis of Next Generation Sequencing Data.

... the analysis, but this time allowing for 2 and 4 cores (commands used are found in Additional file ...simple analysis. In a more complex analysis setting such as SAF estimation we would expect a ...

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Genome analysis pipeline for next-generation sequencing

Genome analysis pipeline for next-generation sequencing

... There were two main aims in this thesis. First, to create a pipeline which can be used to analyse genomic sequencing. Second, to use the pipeline to compare whole human exome capture methods from two ...

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Ultrasensitive single genome sequencing: accurate, targeted, next generation sequencing of HIV 1 RNA

Ultrasensitive single genome sequencing: accurate, targeted, next generation sequencing of HIV 1 RNA

... Results PCR efficiency, and template sampling First, we compared amplification efficiency result- ing from 3 independent experiments in which a sin- gle preparation of cDNA was synthesized using primer IDs, divided and ...

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Parallelization, scalability, and reproducibility in next generation sequencing analysis

Parallelization, scalability, and reproducibility in next generation sequencing analysis

... Using preprocessed indexes, ALPACA avoids redundant computations upon the addi- tion of new samples to an existing study and allows to calculate queries within seconds, providing improved scalability compared to ...

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Introduction to Metagenomics Analysis for Next Generation Sequencing Data

Introduction to Metagenomics Analysis for Next Generation Sequencing Data

... Texas A&M University High Performance Research Computing – https://hprc.tamu.edu Assembly • Velvet, MetaVelvet, MetaVelvet-SL • IDBA-UD • MetAMOS pipeline: selecting [r] ...

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Next generation sequencing (NGS)

Next generation sequencing (NGS)

... LookSeq 19 and Gap5 use the vertical-axis position to indicate insertion size. This places inconsistent mate pairs at the extremes of the plot and visually separates large insert sizes, which are con- sistent with ...

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Reducing ligation bias of small RNAs in libraries for next generation sequencing.

Reducing ligation bias of small RNAs in libraries for next generation sequencing.

... that sequencing cDNA libraries generated by RNA ligases by NGS is an effective ap- proach to study preferences of RNA ...This analysis may also shed light on the in vivo function of Rnl2, ...

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Next Generation Approach of Biological Data Analysis through  in silico Prediction and Analysis of Small ncRNA-RNA

Next Generation Approach of Biological Data Analysis through in silico Prediction and Analysis of Small ncRNA-RNA

... and small ncRNAs present in six healthy human ...interaction analysis helps to explore if there are any specific RNA-RNA interactions or differentially expressed sncRNAs that may be ...of ...

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Next generation sequencing: recent applications to the analysis of colorectal cancer

Next generation sequencing: recent applications to the analysis of colorectal cancer

... actual next-generation sequencing, a lot of work has been done trying to con- sider the “individual variability” to move to the “personal- ized ...

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Analysis of error profiles in deep next generation sequencing data

Analysis of error profiles in deep next generation sequencing data

... deep sequencing. In this study, we performed a comprehensive analysis of the substitution errors in deep sequencing data using the conventional NGS ...deep sequencing and discov- ered that the ...

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Analysis of the mitochondrial DNA of Schizophrenia patients by next generation sequencing

Analysis of the mitochondrial DNA of Schizophrenia patients by next generation sequencing

... ANALYSIS OF THE MITOCHONDRIAL DNA OF SCHIZOPHRENIA PATIENTS BY NGS 5 1.1. SCHIZOPRENIA Schizophrenia is a severe mental disorder characterized by a breakdown of thought processes and by a deficit of typical ...

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