Sodium bisulfite
Sodium Bisulfite as SO3 Source for Synthesis of Methyl Ester Sulfonate Using RBD Stearin as Raw Material
... optimal condition of sulfonation process using falling film reactor. In this research was obtained optimal temperature at 90 0 C and mole ratio between sodium bisulfate and methyl ester was 1.6 : 1 giving active ...
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A systematic evaluation of whole genome amplification of bisulfite-modified DNA
... of bisulfite-modified DNA We performed multiple displacement amplification (MDA)-based WGA by phi29 DNA polymerase using sodium bisulfite-modified DNA as the ...WGA, bisulfite- modified DNA ...
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Chemically induced DNA hypomethylation in breast carcinoma cells detected by the amplification of intermethylated sites
... sis of tumour samples [39]. Our laboratory has adopted this protocol for the methylation analysis of chemical exposure in breast cancer cell lines. We used the estab- lished DNA hypomethylation agent 5-azacytidine ...
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The Ester Enolate Claisen Rearrangement. I. Sterochemical Control Through Stereoselective Enolate Formation. II. Construction of the Prostanoid Skeleton
... acid and the resulting solution Ether extraction, 23 including a base wash and a 10% aqueous sodium bisulfite wash, gave the crude epoxy ester 46 which was purified by medium-pressure ch[r] ...
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Profiling genome-wide DNA methylation
... WGBS (BS-seq; MethylC-seq) theoretically covers all the C information [51]. In this method, genomic DNA is purified and sheared into fragments. The fragmented DNAs are end-repaired; adenine bases are added to the 3 ′ end ...
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Promoter methylation correlates with reduced NDRG2expression in advanced colon tumour
... Bisulfite modification of DNA from colon cancer cell lines, peripheral blood and frozen tissues of patients was assayed, as reported by Herman et al. [18]; normal lym- phocytes (NL) and in vitro methylated DNA ...
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Methylation of Transcription Factor Binding Sites in the Epstein-Barr Virus Latent Cycle Promoter Wp Coincides with Promoter Down-Regulation during Virus-Induced B-Cell Transformation
... PhosphorImager. Bisulfite genomic ...to bisulfite genomic sequencing ...M sodium hydroxide for 10 min at room temperature, neutralized in ...M sodium acetate, and ethanol ...The ...
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MethylPurify: tumor purity deconvolution and differential methylation detection from single tumor DNA methylomes
... genome bisulfite se- quencing (WGBS) and reduced representation bisulfite sequencing (RRBS) [15-18] are popular techniques to pro- file genome-wide methylation at a nucleotide resolution ...The ...
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Evaluating the composition and processing potential of novel sources of Brazilian biomass for sustainable biorenewables production
... Figure 9 shows the effects of different pretreatments on sugarcane bagasse, compared to raw material. A sam- ple obtained from hot water pretreatment (Figure 9b) showed a similar surface to that obtained for raw ba- ...
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Methodological assessment of the reduction of the content of impurities in nimodipine emulsion via the use of 21 amino acid protection
... phase. Sodium bisulfite, sodium thiosulfate, sodium metabisulfite and amino acids are water- soluble antioxidants and have been taken into consideration in this research as stated in the The ...
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Epigenetics mechanisms and degenerative diseases
... of bisulfite-DGGE [denaturing gradient gel electrophoresis] and uses nested PCR ...a sodium-bisulfite-dependent, fluorescence-based, real-time PCR method which sensitively detects and quantifies the ...
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A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples
... the bisulfite reactions at 95°C (24) every 3 h, thereby aiming to maintain complete DNA dena- turation; (4) reducing the DNA quantity to as little as 100 ng (13); (5) increasing the sodium bisulfite ...
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Nitric Oxide: Probably the in Vivo Mediator of the Bisulfite’s Effects
... of bisulfite in food and beverage preservation, as well as in commercial goods and phar- maceuticals as antimicrobial agents is well known, but not very much is reported on its action in ...the bisulfite in ...
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Asymmetric DNA methylation of CpG dyads is a feature of secondary DMRs associated with the Dlk1 / Gtl2 imprinting cluster in mouse
... hairpin bisulfite mutagenesis approach to examine CpG dyad methylation at another secondary DMR (the Gtl2- DMR) as well as at a primary DMR that serves as an ICR (the IG-DMR associated with the Dlk1/Gtl2 imprint- ...
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Region-specific glucocorticoid receptor promoter methylation has both positive and negative prognostic value in patients with estrogen receptor-positive breast cancer
... multiplex bisulfite sequencing assay for detecting GR promoter methylation and used it to investigate the prognostic and predictive significance of GR methylation in a cohort of ER+ patients from a ...
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HAM-TBS: high-accuracy methylation measurements via targeted bisulfite sequencing
... All primers designed for bisulfite PCR were first tested on in vitro methylated DNA to assess amplification effi- ciency and bias. For PCRs within the FKBP5 gene, an in vitro methylated BAC (RP11-282I23, BACPAC) ...
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MethVisual visualization and exploratory statistical analysis of DNA methylation profiles from bisulfite sequencing
... Bisulfite based DNA methylation data can be pro- duced in a high throughput fashion and on the basis of single gene investigation. While high throughput DNA methylation profiling is a very new technological appli- ...
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Methods for analyzing the role of DNA methylation and chromatin structure in regulating T lymphocyte gene expression
... on bisulfite modification of DNA. Treating DNA with bisulfite deaminates cytosine bases to form uracil, but does not affect ...of bisulfite treated DNA allows precise quantitation of the methylation ...
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“Development and Validation of Stability Indicating UFLC Method for the Estimation of Cefotaxime Sodium and Diclofenac Sodium in Bulk and Pharmaceutical Dosage Forms” by Venkata Sairam Koganti, J Channabasappa Thejaswini, Ravandur Shivanna Chandan, Bannimath Gurupadayya, India.
... Cefotaxime sodium and Diclofenac sodium were pipetted into a series of 10 mL volumetric ...Cefotaxime sodium and Diclofenac sodium and are described by regression ...
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PubMedCentral-PMC5789768.pdf
... representation bisulfite sequencing was performed as previously described ...NEXTFlex Bisulfite-sequencing library prep kit (Bioo Scientific, cat# NOVA-5119-02) with additional use of methylated NEXTFlex ...
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