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specific primer

Microarrays for Genotyping Human Group A Rotavirus by Multiplex Capture and Type Specific Primer Extension

Microarrays for Genotyping Human Group A Rotavirus by Multiplex Capture and Type Specific Primer Extension

... microarray. Specific primer extension of the type-specific capture oligonucleotides was applied to incorporate the fluorescent nucleotide analogue cyanine 5-labeled dUTP as a detectable ...

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DETECTION OF ALTERNARIA ALTERNATA ISOLATED FROM GRAIN BY USING SPECIFIC PRIMER

DETECTION OF ALTERNARIA ALTERNATA ISOLATED FROM GRAIN BY USING SPECIFIC PRIMER

... be specific to avoid problems such as detection organism under study as another one, it is very important to monitor and quantify the occurrence of plant pathogen for efficient management of plant ...

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Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs

Blocking primers to enhance PCR amplification of rare sequences in mixed samples – a case study on prey DNA in Antarctic krill stomachs

... species specific primer next to a binding site of a universal primer is often difficult, a krill specific blocking primer, Short28SF-DPO-blkKrill overlapping with the 3'end of the ...

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Pungency Genotyping Of Commercially Cultivated Chilli Pepper Capsicum Species Using Map1 And Csy 1 Molecular Markers

Pungency Genotyping Of Commercially Cultivated Chilli Pepper Capsicum Species Using Map1 And Csy 1 Molecular Markers

... In this study, we develop detection/segregation of pungent and non-pungent in 12 commercial chilli peppers,C. annuum varieties G-3, G-4, G-5, G-273, LCA-335 (G and LCA-series), Pragna and Teja (W-series), Badiga-1, ...

5

Optimization of PCR–RFLP Directly from the Skin and Nails in Cases of Dermatophy-tosis, Targeting the ITS and the 18S Ribosomal DNA Regions

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... dermatophyte specific primer on the Mva I and Hae III enzymes showed an identical band size consis- tently and with the Dde I enzyme, it showed no recognition site on the dermatophytes which were ...phyte ...

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A droplet digital PCR detection method for rare L1 insertions in tumors

A droplet digital PCR detection method for rare L1 insertions in tumors

... Genomic DNA from fibroblast cell lines was extracted using the DNEasy Blood and Tissue Kit (Qiagen; Germantown, MD, USA). The 5’ junction ddPCR assays were performed in 20-μL reactions using ddPCR Supermix for Probes ...

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Citrus Bent Leaf Viroid from Fars Province of Iran

Citrus Bent Leaf Viroid from Fars Province of Iran

... Reverse transcription polymerase chain reaction. Conducting the reverse transcription polymerase chain reaction with a specific primer of citrus bent leaf viroid led to amplification of 230 nucleotides ...

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Mega-introns in the Dynein Gene DhDhc7(Y) on the Heterochromatic Y Chromosome Give Rise to the Giant Threads Loops in Primary Spermatocytes of Drosophila hydei

Mega-introns in the Dynein Gene DhDhc7(Y) on the Heterochromatic Y Chromosome Give Rise to the Giant Threads Loops in Primary Spermatocytes of Drosophila hydei

... exon-specific primer pairs, in combination with hybridization experiments with exon- and satellite- specific probes on filters with large PFGE-generated DNA fragments, offer a simple solution for the ...

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An improved allele-specific PCR primer design method for SNP marker analysis and its application

An improved allele-specific PCR primer design method for SNP marker analysis and its application

... of specific primer are still challenges [27]. The Allele-specific PCR method was developed for allele analysis of clinically significant muta- ...

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Evaluation of a Modified Single Enzyme Amplified Fragment Length Polymorphism Technique for Fingerprinting and Differentiating of Mycobacterium kansasii Type I Isolates

Evaluation of a Modified Single Enzyme Amplified Fragment Length Polymorphism Technique for Fingerprinting and Differentiating of Mycobacterium kansasii Type I Isolates

... adapter-specific primer that has an extension of one selective base at the 3⬘ end, and (iv) electrophoretic separation on an agarose gel and detection of the amplified fragments by ethidium bromide ...

5

DNA Extraction from Beef Harboring Sarcocystis spp.: Comparison of Three Different Analytical Methods

DNA Extraction from Beef Harboring Sarcocystis spp.: Comparison of Three Different Analytical Methods

... For selection of the beef harboring the protozoan Sarcocystis spp., molecular detection was carried out using the specific primer pair of SAR-F: 5'- TGGCTAATACATGCGCAAATA-3' and SAR-R: 5'- ...

6

Tetra Primer ARMS PCR Optimization to Detect Single Nucleotide Polymorphism of the KLF14 Gene

Tetra Primer ARMS PCR Optimization to Detect Single Nucleotide Polymorphism of the KLF14 Gene

... GWAS (genome-wide association studies) have associated Type 2 Diabetes Mellitus with the single nucleotide polymorphism (SNPs) rs972283 (A/G) of KLF14 gene with in a global population. The most common techniques used to ...

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Development of nested polymerase chain reaction detection of Mycosphaerella spp  and its application to the study of leaf disease in Eucalyptus plantations

Development of nested polymerase chain reaction detection of Mycosphaerella spp and its application to the study of leaf disease in Eucalyptus plantations

... individual primer pair, designed to detect Mycosphaerella ...highly specific for their respective targets (Fig. 4B to F). The specific primer pairs verified the taxa of 3 to 15 isolates of ...

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Development and Validation of a Multiplex PCR for Detection of Scedosporium spp  in Respiratory Tract Specimens from Patients with Cystic Fibrosis

Development and Validation of a Multiplex PCR for Detection of Scedosporium spp in Respiratory Tract Specimens from Patients with Cystic Fibrosis

... species-specific primer pairs to detect and identify clinically important Pseudallescheria/Scedosporium species in respiratory (sputum) ...the primer design due to its presence as a multicopy gene in ...

5

Bead based assays to simultaneously detect multiple human inherited blood disorders associated with malaria

Bead based assays to simultaneously detect multiple human inherited blood disorders associated with malaria

... allele specific primer extension (ASPE) to simultaneously detect four epidemiologically important human markers including G6PD SNPs (G202A and A376G) and common haemoglobin mutations (HbS and ...

9

Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method.

Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method.

... A second amplification employing a nested provirus-specific primer and a biotinylated nondegenerate primer derived from the partly degenerate primer followed by purification with strepta[r] ...

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Differentiation of Campylobacter jejuniSerotype O19 Strains from Non O19 Strains by PCR

Differentiation of Campylobacter jejuniSerotype O19 Strains from Non O19 Strains by PCR

... PCR specific for O19 or non-O19 ...as specific primers, either no PCR product was amplified or the O19 and non-O19 strains were not completely differentiated, even with variation in annealing ...

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A PCR-based Method for Isolation of Genomic DNA Flanking a Known DNA Sequence

A PCR-based Method for Isolation of Genomic DNA Flanking a Known DNA Sequence

... primers specific for given restriction enzyme recognition sequences, in conjunction with a specific primer derived from the known DNA sequence (5), whereas Targeted Gene Walking is based on PCR ...

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PCR enzyme linked immunosorbent assay and partial rRNA gene sequencing: a rational approach to identifying mycobacteria

PCR enzyme linked immunosorbent assay and partial rRNA gene sequencing: a rational approach to identifying mycobacteria

... Recently, other probe-based assays capable of identifying a more-restricted range of mycobacteria have been described (1, 18). One of these methods, oligonucleotide-specific capture plate hybridization (OSCPH), ...

6

Analisis talian penghantaran kuasa sistem DWI Litar tiga fasa dan sistem enam fasa

Analisis talian penghantaran kuasa sistem DWI Litar tiga fasa dan sistem enam fasa

... Arus primer Bintang Delta Songsangan Bintang Voltan Fasa Primer Voltan Talian Primer Voltan Fasa Sekunder Voltan Talian Sekunder Voltan Talian Gelung Primer Voltan Fasa Gelung Primer Vol[r] ...

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