Structural and biochemical analysis
Identification of a C-Terminal Regulatory Motif in Hepatitis C Virus RNA-Dependent RNA Polymerase: Structural and Biochemical Analysis
... Our biochemical data confirmed that the C-terminal region, comprising residues 545 to 564, interfered with RNA binding and consequently affected the observed rate of product forma- tion as shown in several ...
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Biochemical and Structural Analysis of Isolated Mature Cores of Human Immunodeficiency Virus Type 1
... for structural and biochemical ...Detailed analysis of 155 cores revealed an average length of ...Immunoblot analysis of core preparations allowed semiquantitative estimation of the relative ...
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Biochemical and structural analysis of thermostable esterases
... and biochemical properties of a novel thermostable esterase containing an Ig-like domain fragment Phe112-Leu117, it is possible that upon substrate binding, Phe116 could change its conformation and open the tunnel ...
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Structural and biochemical insights into the function and evolution of sulfoquinovosidases
... the structural effects of their ...this analysis to the Q262- Q288/K245-E270 pairing proximal to the active site reveals that individual mutation at each position results in an energetic penalty for ligand ...
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Structural and biochemical insights into the function and evolution of sulfoquinovosidases
... the structural e ff ects of their ...this analysis to the Q262-Q288/K245-E270 pairing proximal to the active site reveals that individual mutation at each position results in an energetic penalty for ligand ...
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Structural and biochemical insights of CypA and AIF interaction
... Furthermore, a substitution of the catalytic residue H126 24 in CypA(121-131) with alanine, as expected from the CSP-data, did not affect significantly CypA binding for both the peptide and the full-length protein, ...
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Structural and biochemical studies on the biosynthetic pathways of cyanobactins
... SDS-PAGE Analysis of PatGox Non-Bound Material: SDS PAGE analysis of the flowthrough (non-bound) sample of a His-tag pull-down experiment showing a significantly sized band corresponding to ...
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Structural and biochemical characterisation of pleckstrin homology domains
... 1993). Analysis of cells expressing dominant-negative mutants revealed an accumulation of clathrin-coated pit structures at the plasma membrane and long membrane tubules, consistent with the defects seen with ...
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Paramagnetism & structural biology : biochemical & biophysical analysis of imp-1 metallo-beta-lactamase
... Abstract Resistance to β-lactam antibiotics by pathogenic bacteria is a global concern. Typically arising between 2 and 3 years after the introduction of the antibiotic into clinical use, it is usually due to β-lactamase ...
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Bioinformatics analysis of the structural and biochemical effect of the chemical weapon mustard gas on the P53 protein and cell death
... Bioinformatics analysis of the structural and biochemical effect of the chemical weapon mustard gas on the P53 protein and cell ...of analysis of primary databases) and aggregate of many ...
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Structural and Biochemical Studies of the Carboxyltransferase Domain from Pyruvate Carboxylase
... To investigate the contribution of individual domains to the asymmetrical arrangement of RePC, I removed the biotin carboxylase and biotin carboxyl carrier protein domains from RePC. The resulting recombinant protein ...
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Functional, biochemical and structural analyses of two plasmodium membrane proteins
... [81] 2.6.5 Statistical analyses Statistical analyses were carried out using SPSS (PASW Statistics, now IBM, Portsmouth, UK). To compare the means of data sets the one-way ANOVA (analysis of variance) was used. A ...
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Structural and Biochemical Characterization of Ligand Bound States of the FeMo-Cofactor of Nitrogenase
... dependent analysis of the Se- labeled MoFe-protein required the development of crystallographic sample preparation involving freeze-quench of acetylene reduction assays at specific time ...
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Structural and biochemical studies on tailoring enzymes involved in ribosomal peptides biosynthesis
... Kinetic Analysis of Active Site Variants with Substrate Arg-Az 3 The ternary structures reported here reveal several residues potentially important for catalysis or substrate binding in the vicinity of the bound ...
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Structural and Biochemical Characterization of SbnC as a. Representative Type B Siderophore Synthetase
... Figure S3. Gel filtration chromatographic analysis of SaSbnC. The elution curves of SaSbnC and the protein standards are drawn as a red line and a gray dotted line, respectively. The molecular weight of SaSbnC is ...
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Invariants and Other Structural Properties of Biochemical Models as a Constraint Satisfaction Problem
... Results: In our case, this analysis brings both qualitative and quantitative information on the models, in the form of conservation laws, consistency checking, etc. thanks to finite domain constraint programming. ...
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Biochemical and structural characterisation of a thermophilic Aldo-Keto Reductase fromThermotoga maritima
... SDS-PAGE analysis shows that at 80°C a large proportion of the AKR remains but at higher temperatures and longer times the AKR is slowly denatured without significantly removing extra ...
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CYTOLYTIC T CELL GRANULES Isolation, Structural, Biochemical, and Functional Characterization
... Purity of granules was evaluated by SDS polyacrylamide slab gel analysis (Fig. 3), by morphological examination of the granule fraction by electron microscopy (Fig.[r] ...
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Methods for integrated biochemical pathway analysis
... Process); structural constituent of cell wall (Molecular Function); extracellular region, nucleus, chromosome, chromatin, and nucleosome (Cellular Component) (Table C ...flow analysis cannot check the ...
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Interstrain Variation of the Major Internal Structural Component (p30gag) of Two Murine Oncornaviruses: Comparative Immunochemical, Biochemical, and Biophysical Analysis
... For analogous proteins from two strains Y-1 and Moloney virus p30 proteins share two of murine oncornavirus possessing group-spe- major radioiodinated tryptic peptides. However, cific cr[r] ...
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